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Research on axenic purification of Dunaliella salina

TANG Ying,WANG Chang-hai,LI Ke-jin,HUANG Di(School of Life Science and Biotechnology,Dalian University of Technology,Dalian 116024,China)  
The appropriate antibiotics for Dunaliella salina purification were initially determined by investigating the effects of several antibiotics on the microalgae and co-occurring bacteria.With the mixture of ampicillin,gentamycin sulfate,cefalotin sodium and streptomycin added in the culture,800 μg/mL final concentrations respectively,every two days and repeated three times,the bacteria-free algal culture was obtained by spread plate.The microalgae mixed with bacteria grew a little faster than axenic microalgae,but was aging earlier.The microalgae was stimulated to grow faster with isolated co-occurring bacteria added.The results show significance for further study on relationships between microalgae and co-occurring bacteria,as well as genetic engineering.
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