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Isolation and molecular biological analyses of genomic DNAs from fungi using benzyl chloride

ZHANG Li li,ZHANG Ling hua,SHI Jian fei,WANG Yun ji (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116001,China)  
Benzyl chloride were used for extrcting fungal DNA.Under the condition of pH9.0 and incubated at 50?℃ for an hour after benzyl chloride was mixed enough with fungal cells, Genomic DNAs of Rhizopus chinensis Rc1 and Rhizopus arrhizus Ra1 were extracted and isolated from their integrated cells and from the mycelial powder of Aspergillus ficuum AS3.324 and Trichosporon ficuum Tf1 grinded in liquid nitrogen with high yield and little contamination of protein or other organic components.Molicular sizes of all the isolated DNAs were higher than 23kb.After PCR was operated by using the isolated genomic DNAs of AS3.324 as template and using a double of specifical primers, a 1.4 kb segment of phyA was obtained.
【CateGory Index】: Q781
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