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《Journal of Dalian Institute of Light Inoustry》 2002-01
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Method of protein removal in cordyceps polysaccharide

LIU Ling,AN Jia yan,JIN Feng xie (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116034, China)  
In this paper, the method of protein removal in the course of extracting cordyceps militaris is described. There are a lot of protein in extracting expolysaccharide and inpolysaccharide, Ration and removing protein are important problems. Based on orthogonal experiment, we find the optimum condition for remving protein from cordyceps polysaccharide: matching of exponent/chloroform butanol, 1∶24; volume matching of chloroform butanol,is 1∶0.2; abstracting time, 5?min. Polysaccharide rate obtained is 50%, protein rate removed is 97%.
【CateGory Index】: TQ461
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【References】
Chinese Journal Full-text Database 10 Hits
1 ZHU Ya-Hong1 YAN Hui1 GUI Zhong-Zheng1,2(1School of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China);A Study on the Protective Effect of Cordyceps militaris Polysaccharide to the Primary Cultured Rat Hepatocytes Injured by CCl_4[J];Science of Sericulture;2009-01
2 LIU Cheng-rong (Department of Environment and Life Sciences,Putian College,Putian Fujian 351100,China);A Study on the Technology of Polysaccharide Extracting of Hypsizigus Marmoreus Fruitbody[J];Sichuan University of Arts and Science Journal;2008-05
3 Shi De-fang1,Yang Yang2,Sun Xiao-xue1,Ren Hong1,Han Shu-qin1 (1.College of Light Industry and Food Engineering,2.College of Life Science,Guangxi University,Nanning 530004);Comparison of three Methods of Removing Protein from Polysaccharide Extract in the Cactus[J];Modern Food Science and Technology;2006-04
4 CHENG Hong-weiZHOU Bing-binZHU Yun-lanLIU Quan-de(Xuzhou Institute of Technology, Xuzhou, 221008, China);Study on the Optimum Conditions about Submerged Fermentation for Polysaccharide of Cordyceps and Composition of Polysaccharide[J];;2005-01
5 SHI De-fang,SUN Xiao-xue,REN Hong,HAN Shu-qin (College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,Guangxi,China) YANG Yang (College of Life Science,Guangxi University,Nanning 530004,Guangxi,China);Comparison study on three methods of removing protein from extracted polysaccharide of cactus[J];The Beverage Industry;2006-10
6 YANG Rong-ling1,2,CHEN Wei-dong2,WU Xiao-yu1,LIU Xue-ming2, (1.Department of Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China;2.Sericulture and Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Food, Ministry of Agriculture, Guangdong Open Access Laboratory of Agricultural Product Processing, Guangzhou 510610, China);Extraction Technology Optimization for Extra-cellular Polysaccharide(EPS) of Cordyceps brasiliensis[J];Food Science;2007-07
7 XIANG Yue,TANG Fang-rong,CHANG Yan,LI Ting-ting,TENG Li-rong,CHEN Ya-guang*(College of Life Science,Jilin University,Changchun 130012,Jilin,China);OPTIMIZATION OF THE WATER EXTRACTION TECHNIQUE OF CORDYCEPS MILITARIS MYCELIUM POLYSACCHARIDES[J];Food Research and Development;2008-08
8 WENG Liang1,2, WEN Lu2*, YANG Fang3, WANG Jing-jian2 (1.College of Food Science, Xinjiang Agricultural University, Urumchi 830052; 2.The Resources Microorganism Research Institute of Huaiyin Teachers College, Huaian 223300; 3.College of Life Science, Nanjing Normal University, Nanjing 210097);Empirical study of different extraction methods influence on the antioxidation of polysaccharide in cordyceps militaris[J];Food Science and Technology;2008-11
9 SUN Xiao-xue1*,SUN Wei-dong1,SHI De-fang2,ZHANG Ye-hui11Institute of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;2Hubei Academy of Agriculture Sciences,Wuhan 430064,China;Protein Removal from Polysaccharide Extract of Cactus[J];Natural Product Research and Development;2007-01
10 ZHANG Jing-fang~1,ZHAO Xiao-juan~1,SHI Li-mei~2(1.College of Forestry,Northwest A & F University,Yangling,Shaanxi 712100,China;2.Department of Environment Supervise & Measurment,Xingping,Shaanxi 713100,China);Extracting Technology of Polysaccharides from Persimmon and Constituents Analysis[J];Journal of Northwest Forestry University;2005-04
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Ji Hui, Tu Honghu, Li Naisan Department of Pharmacology;Studies on Extraction and Hypoglycemic Activity of Polysaccharides from Cultural Mycelium of Cordyceps sinensis[J];Journal of China Pharmaceutical University;1993-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Guan min, LIN Sheng qing, HUANG Zong xiu, CHEN Ren xiong, HE Ling, JIANG Rui chai (Fujian Provincial Health and Anti epiodemic Station, Fuzhou 350001, China);STUDY ON THE ANTIMUTAGENICITY OF LENTINAN[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-02
2 SUN Yi-yang1,LI Yang-xia1, GAO Qian1,ZHAO Run1, PAN Yin-jie2(1. Life Science College of Anhui University,Hefei 230039,Anhui,China;2.Edible Fungi Institute, Shanghai Academy of Agricultural Sciences Key Laboratory for Edible Fungus Genetics & Breeding Ministry of Agriculture, Shanghai 201106,China);The Antagonistic Effects of Mycelium Polysaccharides from Agaricus Blazei on Damaged by Cyclophosphamide Toxicity[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-03
3 Liu Zongbin Sun Lizheng (Wuhu Education College Wuhu 241000);Rare-Earth Effect on Brewer's Yeast[J];JOURNAL OF ANHUI INSTITUTE OF MECHANICAL AND ELECTRICAL ENGINEERING;1999-02
4 LU Wen-yun et al (College of Chemistry and Environment, Guizhou University for Nationalities, Guiyang, Guizhou 550025);Compared Analysis of Total Alkaloids and Polysaccharides in Tubing Shoot and Wild Plant of Dendrobium Loddigesii[J];Journal of Anhui Agricultural Sciences;2006-08
5 HU Wei-sheng et al (Food Science and Engineering College,Ocean University of China,Qingdao,Shandong 266003);Measurement of Nine Kinds of Organic Acids in Wort and Beer by Ion Chromatography[J];Journal of Anhui Agricultural Sciences;2007-04
6 WANG Fei et al(College of Bioscience and Engineering,Jiangxi Agricultural University,Nanchang,Jiangxi 330045);Study on Liquid Fermentation Medium for Codyceps militaris by Response Surface Method[J];Journal of Anhui Agricultural Sciences;2007-08
7 WU De-feng, ZHEN Zi-hong, ZHANG Yi, FANG Ce(Tumor Research Laboratory. Fujian Medical Collage)LI Chang-cun (Department of Pharmacology. Fujian Medical Collage);INHIBITION OF HUMAN UTERUS CANCER CELL LINE BY CORDYCEPIN AND PAECILOMYCES SINENSIS IN VITRO[J];Chinese Journal of Cancer;1986-04
8 LUO Hui ling1* , CAI Ti yu1, CHEN Qiao lun1, HUANG Min quan2, MEI Cheng en1, LI Yong qiang1 1.Cancer Institute,Cancer Center, Sun Yat sen University of Medical Sciences, Guangzhou 510060, P.R.China 2.South China Institute of Botany,the;Enhancement of dendrobium candidum polysaccharide on killing effect of LAK cells of umbilical cord blood and peripharal blood of cancer patients in vitro[J];Chinese Journal of Cancer;2000-12
9 GAO Jian~1, XU Xiao-feng~1 , WU Hui-ling~1, CHEN Xue ping~2, ZHOU Jia-hao~3(1. College of Life Science, Nanjing Normal University, Nanjing 210097; 2. College of Economics and Technology,University of Science and Technology of China,Hefei 230052; 3.Zhong-ao Environment Protection Co.Itd,Xuancheng 242000);Construction of Full-length cDNA Library from A NativeTobacco Germplasm and Its Quality Analysis[J];Journal of Anhui Agricultural University;2004-01
10 RUI Hai-yun~1,WU Guo-rong~(2*),CHEN Jing-yao~2,LU Chang-mei~2(1.Department of Biology,Taizhou Teacher′s College,Taizhou 225300;2.College of Life Science,Nanjing Normal University,Nanjing 210097);Isolation,Purification and Structural Characterizationof Neutral Heteropolysaccharide from Bletilla striata[J];Journal of Anhui Agricultural University;2004-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LIU Cheng-rong (Department of Environment and Life Sciences,Putian College,Putian Fujian 351100,China);A Study on the Technology of Polysaccharide Extracting of Hypsizigus Marmoreus Fruitbody[J];Sichuan University of Arts and Science Journal;2008-05
2 PU Yue-wu et al(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou,Guangdong 510006);Study on Antioxidative Activities of Maca Polysaccharides[J];Journal of Anhui Agricultural Sciences;2009-28
3 LIU Hong-jing1,JIANG Ning1,LI Jian-jun1,LIAN Yun-lan2,ZHANG Ping1 (1.Institute of Agricultural Products Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.Shanxi Provincial Institute for Drug Control,Taiyuan 030001,China);Study on Extraction and Purification of Polysaccharides from Cordyceps militaris[J];Acta Agriculturae Jiangxi;2007-12
4 SONG Jiang-feng,LI Da-jing,LIU Chun-quan (Institute of Farm Products Processing,Jiangsu Academy of Agricultural Sciences,Engineering Research Center for Agricultural Products Processing,National Agricultural Science and Technology Innovation Center in Eastern China,Nanjing 210014,China);Optimization of Microwave Assisted Extraction of Cultured Cordyceps militaris Polysaccharide Using Response Surface Methodology[J];Jiangsu Journal of Agricultural Sciences;2009-05
5 CHEN An-hui1,CHEN Hong-wei1,2,XIA Cheng-run3,WU Hai-liang1,FAN Mei-zhen1 (1.Anhui Agricultural University,Hefei 230036,China;2.Xuzhou Institute of Technology,Xuzhou 221008,China;3.Guangzhou Taizong Trading Co.Ltd,Guangzhou 510250,China);Analysis of the Effective Components in the Fermentation Mycelia of Several Anamorphic Strains of Cordyceps[J];Xuzhou Institute of Technology;2007-02
6 DU Lin-fan LU Jia-jiong ZHAO Ying-chun CHU Lan-na(College of Light Industry and Food Engineering,Gangxi Universtiy,Nanning 540003,China);Stedy on deproteinization and decoloration in extraction of glycosaminoglycan from endothlium corneum[J];Shandong Food Ferment;2008-04
7 DU Lin-fan1, ZHAO Ying-chun1, MEI Jun2, CHU Lan-na1 (1.College of Light Industry and Food Engineering, Guangxi University, Nanning 540003, China 2. Quality Assurance Division, Zhengzhou President Enterprises CO., Ltd. zhengzhou 450002, China);Study on Deproteinization and Decoloration in Extraction of Glycosaminoglycan from Endothlium Corneum[J];Sichuan Food and Fermentation;2008-06
8 ZHANG Xiao-yan,CHEN Zheng-xing,WANG Li(State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China);Removal of protein from polysaccharide of rice bran[J];Science and Technology of Food Industry;2008-03
9 QIN Wei-dong,MA Li-hua,CHEN Xue-hong,ZHAO Yue (College of Food, Xuzhou Institute of Technology, Xuzhou 221008, China);Study on Extraction and Deprotein of Polysacharide in Ginger[J];Food Science;2008-04
10 MAO Ying-li1, WANG Zhong-min2,*, LI Jin-yu1, LI Wei1, SHI Xiao-yan1 (1.Xin Jiang Agricultural University, Urumqi 830052,? Xinjiang, China; 2.Food and Drug Administration of Xin Jiang Uygur Autonomous Region , Urumqi 830000, Xijiang, China);STUDY ON THE REMOVAL OF PROTEIN FROM POLYSACCHARIDES OF VITIS VINIFERA L.[J];Food Research and Development;2008-04
China Proceedings of conference Full-text Database 1 Hits
1 ZHOUYong,LI Xue,CHEN Li,WANG Qi (College of Traditional Chinese Medicine,Jinlin Agriculture University,Changchun 130118,China);Studied on the Method of Phellinus igniarius' Hyphostroma Polysaccharides Protein-removed[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Yang Ge (Insitute of Shandong University Microbiology Rsearch, in Jinan 273165);The Amino Acids in Natural and Cultural Cordyceps sinensis[J];AMINO ACIDS & BIOTIC RESOURCES;1997-03
2 Ding Rui, et al;Inhibitory Effect of Dongchongxiacao (Cordyceps Sinensis (Berk.) Sacc.) on Mice Bearing Ehrlich Ascitis Tumors[J];Beijing Medical Journal;1981-06
3 Hu Xiaoying, Peng Guoping, Chen Ruyian (Research Institute of Traditional Chinese Medicine, Nanjing University of Traditional Chinese Medicine, 210029);Antagonism of Liquorice toward Arrhythmia Induced by Prepared Aconite Root[J];JOURNAL OF NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE;1996-05
4 Jing Dadao(Shanghai First People Hospital,Shanghai 200080)Qiu Dekai; Xiao Shudong;Zeng Minde(Shanghai Digestive Disease Institute,Shanghai 200001);Effects of Cordyceps Polysacchrides on the Production of IL-2 and IFN-γ and the Expression of Il-2R in Human Peripheral Blood Lymphcytes[J];SHANGHAI J.OF IMMUNOLOGY;1995-06
5 Chen Bo; Jin Ou (Zhejiang Provincial Institute for drug Control (Hangzhou 310004);Analysis of Nucleosines in the Cordyceps Sinensis and Its Fermental Powder by RP-HPLC[J];CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS;1998-02
6 LiGuiren; WanXiuhua;WuQiang;et al(DepartmentofOphthalmology. Affiliated HospitalofWeifamgMedical College.Shandung 261031);Experimental study on antirejection of cordyceps in penetrating keratoplasty of heterograft[J];JOURNAL OF TRADITIONAL CHINESE OPHTHALMOLOGY;1995-02
7 Li Guiren;Zhangshaobin; Ma Chuanxiang;et al(Department of Ophthalmology,Affiliated Hospital of Weifang Medical college, Shandong 261031);Clinical study on antirejection of cordyceps in karatoplasty of heterograft[J];JOURNAL OF TRADITIONAL CHINESE OPHTHALMOLOGY;1996-01
8 Jia Taiyuan(Jia TY),Benjamin HS Lau(Lau BHS)(Central Hospital of Xi'an,Xi'an 710003);The immuno enhancing effect of Chinese herbal medicine Cordyceps sinensis on macrophage J774[J];CHINESE PHARMACEUTICAL JOURNAL;1997-03
9 Li Xiaoming, Dai Ruqin Zhu Qin(Institute of Chinese Materia Medica, China Academy of TCM, Beijing);Component Analysis of the Extracellular Polysaccharide from Mortierella hepiali Chen et Lu sp. nov.[J];China Journal of Chinese Materia Medica;1989-02
10 Liu Jingming, Zhong Yurong, Yang Zhi, Cui Shulian and Wang Fuhua (Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing);Studies on the Chemical Constituents of Cordyceps mililaris (L.) Link.[J];China Journal of Chinese Materia Medica;1989-10
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