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《Journal of Dalian Institute of Light Industry》 2003-02
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Transforming to Pichia pastoris gene encoding mussel adhesive protein

ZHENG Xin, WANG Yunji(School of Bio. & Food Eng., Dalian Inst. of Light Ind., Dalian 116034,China)  
Adhesive protein extracted from mussel is a raw material for highquality adhesive and preservative. This paper presents the gene encoding for a novel kind of mussel adhesive protein from Mytilus sp.JHX2002, and transforming it to Pichia pastoris Gs115. The cloning vector pUC118/msfp1 is digested with EcoR Ⅰ and Not Ⅰ, and then cloned to a vector pPIC9K digested with EcoR Ⅰ and Not Ⅰ. The expressing vector pPIC9K/msfp1 is obtained and transformed into Gs115 with electric impact method. Inspecting with histidine wildness and PCR, we acquire the recombinant yeast of Gs115/msfp1.
【CateGory Index】: Q785
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