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《Journal of Dalian Institute of Light Industry》 2003-02
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Expression of the Inulinase gene from Kluyveromyces marxianus in Pichia pastoris

WANG Jing,ZHANG Linghua,WANG Yunji(Coll. of Bio. & Food Technol., Dalian Inst. of Light Ind., Dalian 116034, China)  
We isolate Inulinase gene inuB1 from Kluyveromyces marxianus Y109 and integrate it into the polycloning site expressed in vector pPIC9K. Experiment results reveal that the Open Reading Frames (ORFs) of inuB1 is 1 665?bp. We find no intron and four potential sites for Nlinked glycosylation in the sequence. The pPIC9K/inuB1 construct is used to transform Pichia pastoris GS115 (His-, Mut+). The gene inuB1 is integrated into the genomic DNA of GS115 by insertion into a single site for recombination, yielding the recombinant GS115/inuB1. Inulinase is induced by methanol.Its apparent molecular weight is 90kDa (SDSPAGE) and specific activity approaches 12.29?U/mg.
【CateGory Index】: Q786
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZU Guo ren,ZHANG Ling hua (Dept. of Food Sci. and Bio. Eng., Dalian Inst. of Light Ind., Dalian 116034, China);Characteristics of inulinase and sequence analysis of inluinase gene from a kind of yeast strain[J];Journal of Dalian Institute of Light Inoustry;2002-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HUANG Ya-dong1,ZHENG Qing1,LI Xiao-kun1,WANG Lin-chuan2,ZHAO Zheng-fen3,HUANG Zi-ran4(1.Biopharmaceutic R&D Center of Jinan University,Guangzhou 510632,China;2.Department of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;3.College of Zhengzhou Animal Husbandry,Zhengzhou 450008,China;4.Department of Sericulture,South China Agricultural University,Guangzhou 510642,China);Cloning of Spike Protein S1 Gene of Avian Infectious Bronchitis Virus H Strain and Its Expression in Pichia pastoris[J];Chinese Journal of Virology;2003-02
2 LIU Yan-li 1 LIU Yong-dong 2 WANG Yan-hui 1 WANG Yun-shan 2 SU Zhi-guo 2 MA Run-yu 1 (1. The Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029; 2. The State Key Lab of Biochemical Engineering, the Institute of Process Engineering of the Chinese Academy of Sciences, Beijing 100080, China);High density culture conditions of recombinant human serum album from Pichia pastoris[J];Journal of Beijing University of Chemical Technology;2003-04
3 ZHANG Li li,ZHANG Ling hua,SHI Jian fei,WANG Yun ji (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116001,China);Isolation and molecular biological analyses of genomic DNAs from fungi using benzyl chloride[J];JOURNAL OF DALIAN INSTITUTE OF LIGHT INOUSTRY;2000-01
4 WANG Jun,WANG Yun ji,ZHANG Ling hua (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116034, China);Effects on properties of engineering phytases by two different endonuclease[J];Journal of Dalian Institute of Light Inoustry;2002-02
5 WANG Chen xiang,ZHANG Ling hua (School of Bio. & Food Eng., Dalian Inst. of Light Ind., Dalian 116034,China);Screening of C. Krusei WZ-001 phytase and its fermentation condition[J];Journal of Dalian Institute of Light Inoustry;2002-03
6 LIANG Yan-Long, WANG Yun-Ji, ZHANG Ling-Hua (School of Bio. & Food Eng., Dalian Inst. of Light Ind., Dalian 116034,China);Breeding of high-yield phytase yeast Candida Krusei WZ-001 by ion implantation[J];Journal of Dalian Institute of Light Inoustry;2002-04
7 JIA Xiang Zhi 1, YUAN Han Ying 2, MA Wen Yu 1, LI Yuan 1, LI Yu Yang 2 1Department of Microbiology, Fourth Military Medical University, Xi'an 710033, China, 2Institute of Genetics, Fudan University, Shanghai 200433, China;Cloning and expression of human lysozyme gene in Pichia pastoris[J];Journal of The Fourth Military Medical University;2001-22
8 ZHANG Lin 1 , SONG An-qiao 2 , WEN Jin-xu 31 Department of Respiratory Disease, 309 Hospital of PLA, Beijing 100091, China; 2 Health Supervision Office of Haidian District of Beijing, Beijing 100080, China; 3 Department of Respiratory Disease, Nanfang Hospital, First Mil-itary Medical University, Guangzhou 510515, China;Expression of recombinant human endostatin in Pichia pastoris and its inhibitory effects on the growth of human lungadenocarcinoma Astc-a-1cells[J];Journal of First Military Medical University;2003-09
9 ZENG Lihui1, POULTON Jonathan E2, LU Liuxin3 (1.Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.Department of Biological Science, the University of Iowa, IA 52242 U.S.A.;3.College of Horticulture, Fujain Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Expression of β-glucosidase in Pichia pastoris expression system[J];;2003-03
10 HUA Hui,ZHOU Si xiang,WANG Zheng rong (West China Medical Center,Sichuan University,Chengdu 610041,China);Heterologous protein expression in pichia pastoris[J];Foreign Medical Biomedical Engineering Fascicle;2003-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Li li,ZHANG Ling hua,SHI Jian fei,WANG Yun ji (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116001,China);Isolation and molecular biological analyses of genomic DNAs from fungi using benzyl chloride[J];JOURNAL OF DALIAN INSTITUTE OF LIGHT INOUSTRY;2000-01
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