Study on fermentation of P.pastoris recombinant engineering saccharomycete
ZHANG Yu-jing, ZHANG Ling-hua, YE Yun-sheng, WANG Yun-ji(Coll. of Bio. & Food Technol., Dalian Inst. of Light Ind., Dalian 116034, China)
Gene engineering saccharomycete with nattokinase activity are screened by using modified fibrin-agarose plate method. Its fibrinolysis activity is detected by fibrin plate method. Five strains, within one hundred of them, with higher fibrinolysis are screened successfully by methanol inducing. Optimum methanol concentration of the P. pastoris recombinant engineering saccharomycete is 2% and the period of time for activity peaks is 96 h.
【CateGory Index】： TQ925.9