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《Journal of Dalian Institute of Light Industry》 2004-04
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Cloning and hetroexpression of bovine interferon γ in pichia pastoris GS115

ZHANG Hong-yan, XU Xin-hua, ZHANG Ling-hua, WANG Yun-ji(School of Biological & Foodstuff Engineering, Dalian Institute of Light Industry, Dalian 116034,China)  
Gene bovIFN-γ encoding bovine γ-interferon from cow is amplified by using RT-PCR. Sequence detection and analysis reveal that the cDNA of bovIFN-γ is 501 bp in length. The putative protein is composed of 166 amino acids and contains 2 potential N-glycosylation sites. There is a signal peptide from the 23 residues of the N-terminus. The expression plasmid vector pPIC9K/bovIFN-γ as transforming yeast is constructed. The pPIC9K/bovIFN-γ is transformed into pichia pastorisGS115 by electroporation, achieving the recombinant yeast GS115/bovIFN-γ.
【CateGory Index】: Q785
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