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《Journal of Dalian Institute of Light Industry》 2007-02
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Application of gas chromatography in identification of bacteria by analyzing fatty acid in cells

CUI Chang-hao,TIAN Jing,XU Long-quanModern Educational Technology Branch,Dalian Polytechnic University,Dalian 116034,China  
An method of gas chromatography by determining the fatty acids in cells was developed for identification of bacteria.The optimum conditions of separation,collection,methylation and gas chromatography were tested.When this method was applied to detect the fatty acids in cells of As1.643,As1.1130,As1.1807,HWS,DSM.6577,16 kinds of fatty acids can be detected.The results showed that different kind of fatty acids occurred in the 5 tested bacteria,which can be used to identifying these bacteria.
【Fund】: 中科院科研创新基金(K2002A12)
【CateGory Index】: Q93-3
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