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《Journal of Dalian Polytechnic University》 2009-03
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Effects of culture conditions on the saponin accumulation in Astragalus membranaceus callus

ZHANG Zong-shen,WANG Zi-qian(School of Biological & Food Engineering,Dalian Polytechnic University,Dalian 116034,China)  
The saponin accumulation in Astragalus membranaceus callus were studied.The saponin is higher when growing on 6,7-V than that growing on MS,and lumination was apt to stimulating saponin synthesis comparing with darkness.The better ratio of NH+4/NO3 for saponin accumulation in Astragalus membranaceus callus on 6,7-V medium is 1∶1(total concentration of N was {60 mmol·L-1),} in which KH2PO4 is 1.5 mmol·L-1 and CaCl2 is 8.97 mmol·L-1.Sucrose showed the stronger ability to induce saponin synthesis than glucose and maltose.The results suggested that the modulation of culture conditions is the better strategy to improve the saponin accumulation of Astragalus membranaceus callus.
【Fund】: 辽宁省教育厅高等学校科学研究资助项目(051072);; 大连市青年科技人才基金资助项目(2006J23JH031)
【CateGory Index】: S567.239
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