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《Journal of Dalian Polytechnic University》 2009-04
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Comparison of two glycosidase from Absidia sp.

WANG Dong-ming,TANG Si-hui,JIN Feng-xie,YU Hong-shanSchool of Biological & Food Engineering,Dalian Polytechnic University,Dalian 116034,China  
Dioscin-glycosidase(RhaD) from Absidia sp.D0d and Astragaloside glycosidase(RhaA) from Absidia sp.A3r was purified.The molecular mass of RhaD was 59 ku.The optimum pH and temperature of RhaD for enzyme activity was 5.0 and 40 ℃ respectively.RhaD was stable at pH 3.0-8.0 and below 60 ℃.K+ and Na+ showed no effect on the enzyme activity,while Mg2+,Zn2+,Ca2+,Cu2+,Fe3+ inhibited the enzyme.The Km and Vmax were 19.26 mmol/L and 1.23 mmol/(L·h).The molecular mass of RhaA was 54 ku.The optimum pH and temperature of RhaA for enzyme activity was 5.0 and 40 ℃ respectively.RhaA was stable at 3.0-6.0,and below 60 ℃.Mg2+ and Ca2+ showed no effect on the enzyme activity,while Cu2+ and Fe3+ inhibited the enzyme.The Km and Vmax were 17.86 mmol/L and 1.18 mmol/(L·h).RhaD can hydrolyze α-rhamnoside of Dioscin but not pNP-α-L-Rha.RhaA can hydrolyze α-rhamnoside of Astragaloside16 but not pNP-α-L-Rha.
【Fund】: 国家自然科学基金资助项目(20476017);; 辽宁省高等学校优秀人才支持计划资助项目(RC-04-05);; 辽宁省科学技术基金资助项目(20042132)
【CateGory Index】: TQ464.8
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