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《Journal of Dalian Fisheries University》 2003-03
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RAPD Analysis of the polymorphism of genomic DNA among three abalones

XING Ronglian1, DING Jun1, TENG Liping2, CHANG Yaqing1, SONG Linsheng3 PRC, Dalian Fisheries Univ., Dalian 116023, China;2.Dalian Laohutan Ocean Park, Dalian 116013, China; 3.Experimental Marine Biology Laboratory Institute of Oceanology,Chinese Academy of Sciences, Qingdao 266071, China)   
Polymorphism of genomic DNA and molecular classification of three species of abalones, Haliotis midae, Haliotis discus hannai, Haliotis diversicolor supertexte,were determined by random amplified polymorphic DNA (RAPD). Amplifications with 24 primers gave 376 reproducible amplified fragments ranging between 250 and 2500 bp in length,the amplified fragments were scored as present(1) or absent(0) for each DNA sample and an index of degree of band sharing(F) was calculated by Nei and Lis matching coefficient method. The value of(1-F) was used to evaluate genetic distances between species. The phylogenetic trees were constructed with the methods of UPGMA and NJ on the basis of genetic distances. UPGMA analysis indicated that there was a close relationship between H. midae and H. diversicolor supertexte, followed by H. discus hannai, and no significant genetic distances between the abalones. RAPD approach provides a new method for efficient and simple classification and identification of new strains for abalones.
【Fund】: 国家自然科学基金资助项目(30200212)
【CateGory Index】: Q953
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