Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Dalian Fisheries University》 2006-01
Add to Favorite Get Latest Update

Detection of Vibrio parahaemolyticus by Dot-ELISA

ZANG Hong-mei~(1,2),FAN Jing-feng~2,WANG Bin~1(1.School of Life Science and Technology,Dalian Fisheries Univ.,Dalian 116023,China;2.National Marine Environmental Monitoring Center,Dalian 116023,China)  
A Dot-Enzyme Linked Immunosorbent Assay(Dot-ELISA) was established for detection of Vibrio parahaemolyticus.The working concentration of serum samples was 1∶200 dilution and that of the enzyme-labeled goat anti-rabbit IgG was 1∶100 dilution by chessboard assay.The samples with clear and obvious dots were judged as positive reaction.V.parahaemolyticus was positive in the Dot-ELISA,while V.harveyi,Aeromonas hydrophila,V.fluvialis biotype,V.alginolyticus and Escherichia coli were negative in the assay.Dot-ELISA was characterized by rapid reaction,economical feasibility,and observable results and widely adopted.
【Fund】: 国家“863”高新技术研究发展计划项目(2002AA639470)
【CateGory Index】: R115
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 LI Guo1,2,YAN Maocang2,CHANG Weishan1,LIU Liansheng3,MA Aimin1,LIN Zhihua2 (1. College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Taian 271018,Shandong,China; 2. Zhejiang Mariculture Reseach Institute,Wenzhou 323005,Zhejiang,China; 3. College of Life Science,Anhui Agricultural University,Hefei 230036,Anhui,China);Study on Indirect ELISA Method for Detecting Vibrio Parahaemolyticus in Hard Clam Meretrix Meretrix Linnaeus[J];Marine Science Bulletin;2008-05
2 XIE Jian-jun1,2,WANG Yin-geng1,ZHANG Zheng1,RONG Xiao-jun1(1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Biology Science&Technology College,Shanghai Fisheries University,Shanghai 200090,China);Rapid detection of two pathogens of skin ulcer syndrome in cultured Apostichopus japonicus by Dot-ELISA[J];Marine Sciences;2007-08
3 WANG Ling1,2 TIAN Shi-Min2 GAO Hai-Xia1,2 ZOU Ming-Qiang2 JIANG Ji-Zhi1 CUI Xia2 HU Xiu-Li2 CHEN Li-Ben2(1.College of Life Science,Hebei University,Baoding 071002)(2.Chinese Academy of Inspection and Quarantine,Beijing 100025);Study on Detection of Pantoea Stwartii subsp.stewartii Based on Dot-ELISA[J];Microbiology;2008-02
China Proceedings of conference Full-text Database 1 Hits
1 FU Ling-lin,LI Jian-rong (College of Food Science and Biotechnology,Zhejiang Gongshang University,Food Safety Key Laboratory of Zhejiang Province,Hangzhou 310035,China);Detection and molecular typing on Vibrio parahaemolyticus in seafoods[A];[C];2008
Chinese Journal Full-text Database 10 Hits
1 Ma Jiahao\ Li Xueqin\ Wang Zhenyin\ Yang Zhenguo\ Wang Wendong (Research Center of the Aquatic Animal Diseases of PLA);Rapid Detection of Motile Aeromonas Species in Fish by Dot ELISA[J];JOURNAL OF DALIAN FISHERIES UNIVERSITY;1997-03
2 XU Bin-fu 1,LIN Neng-feng 1,YANG Jin-xian 1,YU Fu-song 1,DONG Chuan-fu 2,LIN Tian-long 1 (1.Center of Fish Disease Control,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350003,China; 2.Fisheries College, Huazhong Agriculture University,;Isolation,identification and pathogenicity analysis of Vibrio parahaemolyticus from Pseudosciaena crocea[J];Fujian Journal of Agricultural Sciences;2002-03
3 KOU Yun-tong1 MA Hong-ming2 LIU Chen-guang2(1Qingdao Entry-Exit Inspection and Quarantine Bureau of P.R.China, 266002) (2 Ocean University of Qingdao,266003);RAPLD DETECTION OF Vibrio parahaemolyticus BY PCR METHOD IN SEAFOOD[J];Marine Sciences;2002-09
4 ZHOU Yong-Can ZHANG Ben CHEN Xue-Fen QIAN Jia-Ying (Fisheries Department, Hainan University, Haikou, 570228);PRELIMINARY STUDIES ON THE RED BODY DISEASE IN Penaeus vannamei[J];Marine Sciences;2003-05
5 TAO Bao--hua, HU Chao--qun,WU Wei(South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou, 510301 China );STUDIES ON PATHOGENETIC BIOLOGY OF VIBRIOSISOCCURRING IN PENAEUS MONODON[J];Tropic Oceanology;2001-02
6 Shen Yalin and Yu Yeshao (East China Sea Fisheries Research Institute, Shanghai 200090);PATHOGENICITY OF VIBRIO PARAHAE-MOLYTICUS TO HEALTHY CLAM MERETRIX MERETRIX AND THE PREVENTION AND TREATMENT OF VIBRIO INFECTIONS[J];Journal of Fisheries of China;1993-03
7 XU Hai-sheng, SHU Miao-an, ZHAN Xiu-an, Wang Shu-xia (1. College of Animal Science , Zhejiang University , Hangzhou 310029, China ; 2. College of Life Science, Zhejiang University, Hangzhou 310012, China);Identification of Vibrio parahemolyticus isolated from cultured Eriocheir sinensis and pathogenicity of its extracellular products[J];Journal of Fisheries of China;2002-04
8 WANG Zhi-qiang,LI Zhi-yong,Ling Li(Guangzhou Entry-Exit Inspection & Quarantine Bureau, Guangzhou 510623, China);Isolation and Examination of Salmonella and Vibro parahaemolyticusin Meat and Bone Meal[J];Food Science;2004-11
9 Ma GuangGang1), Guo FuSheng2), Wang Juan2), Wang YuDong2), Zhang YanHai2)(1 College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018; 2 Animal Quarantine Iustitute, MOA, Qingdao, 266032);Isolation and Ideutification of Vibrio parahaemolyticus in Sea Products[J];China Animal Quarantine;2002-09
10 ZHANG Hong-jian~1, HAN Zhi-hui~1, WEI Jian-hua~2, ZHAXI Ying-pai~1 (1. College of Agricultural and Animal Sciences, Qinghai University, Xining 810003,China; 2. Qinghai Provincial Consultative Station of Animal and Veterinary Science and Technology, Xining 810008,China);Detection of Salmonella in carcasses of beef by Dot-ELISA[J];Chinese Journal of Veterinary Science and Technology;2004-02
Chinese Journal Full-text Database 10 Hits
1 Ye Jiansheng1,2Wang Xingqiang2*Ma Shen1Yan Binlun2(1 The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China;2 Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China);Advances in Research of Epizootiology and Measures of Sustainable Developmental Breeding in Bivalves[J];Anhui Agricultural Science Bulletin;2007-03
2 JIANG Jin-qing et al (College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Study on Enhancing the Cell Immunity of Broiler with Chinese Herbal Medicine[J];Journal of Anhui Agricultural Sciences;2006-18
3 CHEN Ping et al (College of Animal Science and Technology, Laiyang Agricultural University, Qingdao, Shandong 266109);Effect of Eperythrozoon Wenyonii on Immune Level in Cows[J];Journal of Anhui Agricultural Sciences;2007-26
4 ZHANG Yong et al (College of Veterinary and Animal Sciences,Shenyang Agricultural University,Shenyang,Liaoning 110161);Effect of Trifolium Pratense Oligopeptide on Immune Function of Mouse[J];Journal of Anhui Agricultural Sciences;2007-35
5 ZHOU Zhen-bing et al (Guangxi Agricultural Vocational and Technical College,Nanning,Guangxi 530007);Studies on the Extraction of Polysaccharides from Sargassum and Its Immunological Experiment[J];Journal of Anhui Agricultural Sciences;2009-16
6 Anhui Medical College,Hefei 230061LI Hua ,GONG Chuan ,KUANG Li -xiang;The effects of treatment with XueKang oral solution on immune thrombocytopenia in rabbits[J];Journal of Anhui Health Vocational & Technical College;2003-03
7 CHENG~1 Qi-ping, ZHOU~1 Shou-biao,ZHENG~2 Wei-fa (1.College of Life Science, Anhui Normal University, Wuhu 241000,China; 2. Key Laboratory on Biotechnology for Medicinal Plants of Jiangsu Province, Xuzhou Normal University , Xuzhou 221116,China);Experimental Study on Effects of Anti-tumour of Exopolysaccharides of Aphanothece halophytica (EPAH) in Mice[J];Journal of Anhui Technical Teachers College;2005-01
8 DENG Huan,CHEN Qiu,ZHOU Hong(Liaoning Marine Fisheries Research Institute,Liaoning Open Lab of Applied Marine Biology,Dalian 116023,China);Isolation and Pathogenicity of Vibrio nigripulchritudo From bay Scallop (Argopecten irradians)[J];Fisheries Science;2003-03
9 ZHANG Yue-ling~(1,2), ZHUO Yi-ming~1, ZHU Yong-fei~1, WANG San-ying~2, HUANG Tong-wang~1(1. Department of Biology, School of Science, Shantou University, Shantou 51506or Cell Engineering, College of Life Sciences, Xiamen University, Xiamen 361005, China);Identification of Two Types of Main Differential Proteinsin the Hepatopancreas in Whiteleg Shrimp Litopenaeus vannamei Infected by Pathogenic Bacteria Vibrio alginolyticus[J];Fisheries Science;2005-06
10 HAN Yun,CAI Jun-peng,SONG Zhi-ping,WANG Zhi(College of Food Science and Biotechnology,South China University of Technology,Guangzhou 510640,China);Elimination of Potentially Pathogenic Vibrios in Seafood by Bdellovibrio sp.[J];Fisheries Science;2005-11
China Proceedings of conference Full-text Database 4 Hits
1 Yang Yuanjie Chen Hui (Freshwater Fisheries Research Centre of the Chinese Academy of Fishery Sciences,Wuxi,China,214081) (Jiangsu Provincial Aquatic Technotogy Extension Station,Nanjing,China,210036);Rapid detection of Vibrio anguillarum using Enzyme-Linked Immunosorbent Assay (ELISA)[A];[C];2003
2 ZHANG Cai-hong LI Wen-li REN Hui-ying JIANG Jian-yang ZHU Feng-hua(College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China);Effects of Chromium Yeast on Immune Function and Carcass Quality in Heat Stressed Broilers[A];[C];2008
3 LIU Zi-kui~1 YAO Guo-min~2 WU Ye~1 HU Xing-ping~1 ZHU Zhong-ping~1 (1.Hunnan AgricuLturaL University AnimaL PharmaceuticaL co.;Ltd.Changsha Hunan,410128;2.Huaihua VocationaL and TechnicaL CoLLege,Huaihua Hunan,418000;China);Observation on Immunopotentiation of Compound AstragaLus PoLysaccharides Injection as Immunoadjuvant[A];[C];2008
4 ZhaoGuangying WuYanyan Food College of Zhejiang Gongshang University, Hangzhou 310035;Vibrio parahaemolyticus in foods and the detection[A];[C];2005
Chinese Journal Full-text Database 10 Hits
1 Li Su1, Jiang Wen- Qi1, Wang An- Xun2, Guan Zhong- Zhen1, Pan Shi- Rong2 1.Department of Medicine,Cancer Center,Sun Yat- sen University, Guangzhou,Guangdong,510060, P.R.China 2.Department of Stomatology,The first Affiliated Hospital, Sun Yet- Sen University, Guangzhou,Guangdong,510080, P.R.China 3.Department of Cardiology,The first Affiliated Hospital, Sun Yet- Sen University, Guangzhou,Guangdong,510080, P.R.China;Studies on 5- FU/PEG-PBLG Nano-micelles:Preparation, Characteristics,and Drug Releasing In Vivo[J];Chinese Journal of Cancer;2004-04
2 MAO Zhi juan 1,LIU Guo yong 2,CHEN Chang fu 2 (1 Ningbo Fishery Research Institute,Ningbo 315012; 2 Key Laboratory of Agricultural Microbiology, Ministry of Agriculture);Isolation and Identification of Pathogenic Bacteria Causing Ulcerosis in Large Yellow Croaker (Pseudosciaena crocea)[J];Journal of Anhui Agricultural University;2002-02
3 ZHOU De-qiang,YANG Long-xing(School of Mechanical and Automobile Engineering,Jiangsu Teachers University of Technology,Changzhou 213001,China);Research on Automatic Control System of Environmental Factors in Aquaculture[J];Journal of Jiangsu Teachers University of Technology;2006-04
4 WU Bin1, MA Jie2, LIN Weixuan1(1. Liaoning EntryExit lnspection and Quarantine Bureau of PRC, Dalian 116001, China;2. Colleges of Basic Medical Sciences, Dalian Medical University, Dalian 116027, China);Ioslation and identification of causative vibrio in sea products[J];Journal of Dalian Institute of Light Industry;2003-01
6 WU Zhen-long, NIE Jun, BAO Hui-ning, XU Bing, WANG Hong (Department of Epideminlogy, First Military Medical University, Guangzhou 510515,China ;Tongling People's Hospital of Anhui Province,Tongling 244000, China; Institute of Biotechnology, Academy o f Mi;Cloning of thermostable direct hemolysin gene of Vibrio parahaemolyticus[J];Journal of First Military Medical University;2001-02
7 LI Zhi-Feng, NIE Jun, DAI Ying-Chun, LI Jian-dong, CHEN Qing, YU Shou-yiDepartment of Epidemiology, Southern Medical University, Guangzhou 510515, China;Bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene[J];Journal of First Military Medical University;2004-10
8 LIN Ben-fu, ZHANG Nai-sheng, YUAN Li-hong (Faculty of Military Veterinary, the Quartermaster University of PLA,Changchun,Jilin,130062,China);The Progress of the Capsular Polysaccharide of Staphylococcus aureus[J];Progress In Veterinary Medicine;2005-03
9 XU Bin-fu 1,LIN Neng-feng 1,YANG Jin-xian 1,YU Fu-song 1,DONG Chuan-fu 2,LIN Tian-long 1 (1.Center of Fish Disease Control,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350003,China; 2.Fisheries College, Huazhong Agriculture University,;Isolation,identification and pathogenicity analysis of Vibrio parahaemolyticus from Pseudosciaena crocea[J];Fujian Journal of Agricultural Sciences;2002-03
10 SU Jian xin, NIE Jun, SU Gui dong, et al. Department of Epidemiology, the Military Medical Institute of Guangzhou Command PLA, Guangzhou 510507, China;A Study on Expression and purification of TDH-related hemolysin gene of Vibrio parahaemolyticus[J];Guangdong Journal of Health and Epidemic Prevention;2004-01
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Feng-ping1,2,3 WANG Yin-geng1 LI Sheng-zhong3RONG Xiao-jun1 LIAO Mei-Jie1 ZHANG Zheng1(1Key Laboratory of Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071)(2 Zhoushan Fisheries Research Institute,316000)(3 College of Animal Science, Xinjiang Agricultural University,Wulumuqi 830052);The PCR technique for rapid detection of Vibrio splendidus in Apostichopus japonicus suffering from skin ulcerative syndrome[J];Marine Fisheries Research;2008-05
2 MA Aimin1,2,YAN Maocang1,XIE Qilang1,CHEN Shaobo1,SHAN Lezhou1,CHANG Weishan2(1.Zhejiang Mariculture Research Institute,Wen zhou 325005,China;2.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource,Wenzhou 325005,China;3.Department of Animal Sciences and Technology,Shandong Agricultural University,Tai’an,271018,China);Studies on the Optimum Culture Conditions of Vibrio splendidus M-1[J];Bulletin of Science and Technology;2009-06
3 WANG Yin-geng1,ZHANG Feng-ping1,2,LI Sheng-zhong3,CHEN Xia4,CUI Yu-long5(1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Zhounshan Fisheries Research Institute,Zhoushan 316000,China;3.College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;4.Sunny Oceans Aquaculture Technology Co.,Ltd,Qingdao 266071,China;5.Municipal Department of Ocean and Fishery of Rongcheng,Weihai 264309,China);Detection of Vibrio splendidus causing skin ulcer symdrome of sea cucumber(Apostichopus japonicus) with DNA probes[J];Journal of Fisheries of China;2009-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XU Bin\|fu,LIN Neng\|feng,YANG Jin\|xian,YU Fu\|song,Chen Qiang,LIU Xiao\|dong,LIN Tian\|long (Center of Fish Disease Control,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian\ 350003,China);The trend of Fujian aquatic science development in the new century[J];Fujian Journal of Agricultural Sciences;2000-S1
2 Li Tiandao;Yu Jia; Yu Kaikang(Ocean University of Qingdao, Qingdao, 266003) (Yellow Sea Fisherics Research Institute, Qingdao, 266071);STUDIES ON PATHOGENICITY OF FOUR VIBRIO STRAINS FOR PENAEUS CHINESIS[J];TRANSACTION OF OCEANOLOGY AND LIMNOLOGY;1998-01
3 Wang Lei; Li Guangyou; Mao Yuanxing; Zhang Haiyan (Institute of Oceanology, Academica Sinica, Qingdao 266071);EFFECT OF ORAL IMMUNO DRUGS FOR PREVENTION AND CONTROL OF DISEASES OF CULTURED PENAEUS CHINENSIS[J];Oceanologia Et Limnologia Sinica;1994-05
4 Zhan Wenbin; Zhou Li; Yu Kaikang; Chen Zhangqun; Meng Qinghan(College of Fisheries,Ocean University of Qingdao,Qingdao 266003);VIBRIO GAZOGENES, A NEW VIBRIO PATHOGEN FROM SEPTICEMIA OF PENAEUS CHINENSIS[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1997-01
5 MOU Hai-jin, LI Yun, BAO Zhen-min, YANG Xue-song, XU Hual-shu (College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, 266003) (Shandong Laizhou Da Hua Fisheries Culture Research Institute, Laizhou, 261413);PATHOGENICITY OF EXTRACELLULAR PRODUCTS OF VIBRIO PARAHAEMOLYTICUS TO PENAEUS CHINENSIS[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;2000-03
6 Song Chunhua et al(Weihai Branch of Shandong University, 264200);Studies on Prevention, Treatment and Tissue Pathology of the Red Leg-Disease of Penaeus orientalis Caused by Vibrio parahaemolyticus[J];SHANDONG FISHERIES;1998-03
7 LI Tai wu 1, SU Xiu rong 1, DING Ming jin 2(1.Institute of Marine Biotechnology,Liaoning Normal University, Dalian 116029,China; 2.Dalian Fisheries Company,Dalian 116023,China);Study on Extracellular Products of Vibrio fluvialis Ⅱ, Pathogen of Pustule Disease of Haliotis discus hannai[J];JOURNAL OF LIAONING NORMAL UNIVERSITY(NATURAL SCIENCE EDITION);1999-03
8 HU Chao qun, TAO Bao hua (South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou 510301, China);PENAEID SHRIMP VIBRIOSIS AND IMMUNE PREVENTION: A REVIEW[J];Tropic Oceanology;2000-03
9 LIU Hong WANG Kan PENG Jin xin ZHAO Jun he ZHANG Xiao qing ( Shatoujiao Animal and Plant Quarantine Service, Shenzhen 518081 );Study on Bacterial Pathogen of " Red Body Disease ” in Penaeus monodon[J];FISHERIES SCIENCE & TECHNOLOGY INFORMATION;1999-01
10 Zheng Guoxing;Li He;Huang Ningyu;Yu Yeshao East China Sea Fisheries Research Institute, Shanghai Yang Jifang;Wu Youlu The second Institute of Oceanography, Hangzhou;CHARACTERISTICS OF VIBRIO ALGINOLYTICUS ISOLATED FROM DISEASED CLAM MERETRIX MERETRIX AND HISTOPATHOLOGICAL OBSERVATIONS ON DISEASED CLAM BY ELECTRON MICROSCOPE[J];Journal of Fisheries of China;1991-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved