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《Journal of Dalian Fisheries University》 2006-01
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Detection of Vibrio parahaemolyticus by Dot-ELISA

ZANG Hong-mei~(1,2),FAN Jing-feng~2,WANG Bin~1(1.School of Life Science and Technology,Dalian Fisheries Univ.,Dalian 116023,China;2.National Marine Environmental Monitoring Center,Dalian 116023,China)  
A Dot-Enzyme Linked Immunosorbent Assay(Dot-ELISA) was established for detection of Vibrio parahaemolyticus.The working concentration of serum samples was 1∶200 dilution and that of the enzyme-labeled goat anti-rabbit IgG was 1∶100 dilution by chessboard assay.The samples with clear and obvious dots were judged as positive reaction.V.parahaemolyticus was positive in the Dot-ELISA,while V.harveyi,Aeromonas hydrophila,V.fluvialis biotype,V.alginolyticus and Escherichia coli were negative in the assay.Dot-ELISA was characterized by rapid reaction,economical feasibility,and observable results and widely adopted.
【Fund】: 国家“863”高新技术研究发展计划项目(2002AA639470)
【CateGory Index】: R115
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