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《Journal of Dalian Fisheries University》 2007-03
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The effects of combined Chinese herb medicines on function of immunocytes and resistance to diseases in grass carp Ctenopharyngodon idellus

WANG Bin1,ZHAO Wen1,FAN Wei 1,YIN Shou-ren2,XU Li-pu2,SHENG Zhu-mei2, LI Yuan-yuan1(1.Key Laboratory of Hydrobiology in Liaoning Province's University,Dalian Fisheries Univ.,Dalian 116023,China2.Beijing Fisheries Technical Extension Station,Beijing 100021,China)  
Juvenile grass carp Ctenopharyngodon idellus weighing 150-180 g were fed diets supplemented with two types of combined Chinese herb medicines for one and two weeks,then the phagocytic ability and proliferation of macrophage and lymphocytes were determined by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromid) method and phago-neutral red assay.At this time,grass carp were challenged by Flexibacter calumnaris and safeguard rate was observed.Phagocytic ability and proliferation of macrophage and lymphocytes were investigated in the infected individuals.The results showed that the fish fed the diets containing the compounds of traditional Chinese medicine had significantly higher phagocytic ability and proliferation of macrophage(P0.01),but proliferation of lymphocytes were not significant difference(P0.05).The difference was observed in both compound 2 and compound 4(P0.01).On infective condition,the safeguard rate were 50% with the compound 2 and 14.6% with the compound 4 .The phagocytic ability and proliferation of macrophage and lymphocytes very significantly increased(P0.01).
【Fund】: 北京市科委研发攻关项目资助(Y0705003040291)
【CateGory Index】: R285.5
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