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《Journal of Dalian Fisheries University》 2007-04
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RAPD analysis of genetic diversity for cultured largemouth bass Micropterus salmoides

LIANG Su-xian1,2,BAI Jun-jie1,YE Xing1,LAO Hai-hua1,JIAN Qing1(1.Key Laboratory of Tropical & Subtropical Fish Breeding & Cultivation,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;2.School of Life Science and Technology,Dalian Fisheries Univ.,Dalian 116023,China)  
Random amplified polymorphic DNA(RAPD) method was used to detect the genetic diversity of cultured largemouth bass Micropterus salmoides obtained from Guangdong province.Using 12 random primers,106 RAPD sites were detected,with the average loci of 8.8 in each primer.The Guangzhou,Shunde and Nanshui populations had mean polymorphic loci percentage of 32.08%,33.02% and 40.57%;Nei's gene diversity of 0.1151,0.1176 and 0.1627 and the Shannon's diversity of 0.1708,0.1742 and 0.2378,respectively.These indexes indicate that the cultured largemouth bass from Guangdong province has not decrease in genetic diversity at present, but in some areas has decrease.
【Fund】: 国家科技基础条件平台工作项目资助(2005DKA21103)
【CateGory Index】: S917.4
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