Embryonic development of gynogenetic diploid and triploid Japanese flounder Paralichthys olivaceus
LIU Hai-jin1, WANG Chang-an2,3, ZHU Xiao-chen2,3, LIU Yong-xin4, ZHAHG Xiao-yan4, HOU Ji-lun4,TANG Nan4 (1.Chinese Academy of Fishery Sciences,Beijing 100039,China;2.School of Life Science and Technology,Dalian Fisheries Univ.,Dalian 116023,China;3.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;4.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China )
Embryonic development of haploid,triploid,gynogenetic diploid and normal diploid Japanese flounder(Paralichthys olivaceus) were observed at a water temperature of(16±0.5)℃,pH value of 8.0-8.3,and a salinity of 30.2.A high frequency of abnormality,particularly body flexion,the organ differentiation,the fertilizing rate,the rate of abnormality and the hatching rate was found in the haploid embryos.The embryos of gynogenetic diploid and triploid had basically the same process with the normal diploid embryos,as the zygotes developed through multicelled stage,blastula stage,gastrula stage,neurula stage,yolk plug formation stage,Kupffer's vesicle stage,tailbud stage,eye len formation stage,heart beating stage,hatching stage and hatched successfully.There were no significant differences in embryonic development among gynogenetic diploid,triploid and normal haploid individuals.However,different time of every stage was found in embryonic development among gynogenetic diploid,triploid and haploid individuals.It took about 61 hours to hatch for the control diploid.For gynogenetic diploid and triploid,however,it took 66 hours 30 minutes and 67 hours 50 minutes to hatch,respectively,about 10% longer than the developmental time of the control diploid.Noticeably,the gynogenetic diploid and triploid had about 25% longer than the control diploid at the multicelled stage.The hatching rate of(16.5±2.2)% and abnormity of(97.2±1.6)% happened to the haploid.The hatching rate of(85.6±3.0)% and(82.3±3.1)% occurred in both gynogenetic diploid and triploid with the abnormality of(13.2±2.3)% and(16.3± 2.5)% respectively,no significant differences between gynogenetic diploid and triploid.But compared with the control diploid,both gynogenetic diploid and triploid had a lower hatching rate and higher abnormality.
【Fund】： 科技支撑计划项目(2006BAD01A1207);; 农业科技跨越计划项目(2006跨18);; 农业成果转化资金项目(2006GB23260365)
【CateGory Index】： S917.4
【CateGory Index】： S917.4