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《Journal of Southeast University (Natural Science Edition)》 2004-01
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Preparation and biological properties of porous chitosan scaffolds

Wang Jun 1,2 Li Xinsong 1,2 Zhao Yanqiu 3 Pu Yuepu 3 ( 1Department of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, China) ( 2Laboratory for Biomaterials and Drug Delivery, Southeast University, Nanjing 210096, China) ( 3Public Health College, Southeast University, Nanjing 210096, China)  
Protein modified chitosan was used as a matrix to prepare three dimensional porous scaffolds(protein chitosan scaffold,PCS) by a freeze drying method, and the cytotoxicity, cell adhesion and proliferation of the scaffolds were investigated. The results reveal that porous scaffolds with different structures can be prepared by pre freezing at different temperatures. The PCS show no cytotoxicity. L929 cells and fibroblast cells on the PCS tend to maintain phenotypic shape and grow and proliferate very well. And, normal keratinization can be observed by scanning electron microscape (SEM) on the surface of the PCS.
【Fund】: 江苏省高技术研究基金资助项目 ( 2 0 0 2 0 0 2 );; 江苏省社会发展研究基金资助项目 ( 2 0 0 10 3 4);; 东南大学科学基金资 助项目
【CateGory Index】: Q819
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