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《Journal of Southeast University(Natural Science Edition)》 2008-06
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In vitro study of cytotoxicity of nanosized silica

Yang Hong1,2 Wu Qiuyun2 Tang Meng2 Cao Songyu2 Dong Yuying2 Sheng Jingyi2 Lu Zuhong1,3(1 State Key Laboratory of Bioelectronics,Southeast University,Nanjing 210096,China)(2 School of Public Health,Southeast University,Nanjing 210009,China)(3 Research Centre of Learning Science,Southeast University,Nanjing 210096,China)  
The toxicity effects of nanosized silica and micron silicon dioxide were compared by hemolysis test of erythrocytes,3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide(MTT) assay,cell morphology and detecting lactate dehydrogenase(LDH) in culture medium of RAW264.7 cells.Oxidative stress level indicated by the production of malondialdehyde(MDA),hydrogen peroxide(H2O2),hydroxyl radicals(·OH),superoxide anion radical(O2·-) and nitric oxide(NO) in cells were measured.The results show that after exposure to two kinds of powder within a dose range,the erythrocytes hemolysis rates and concentration of MDA increase in a dose-dependent manner.The inhibitory concentration 50%(IC50)of nanosized silica and micron silicon dioxide is 11.61 and 38.12 mg/cm2 respectively.Meanwhile,ballooning degeneration of cells can be observed by light and fluorescence microscope.The activity of LDH leakage induced by nanosized silica and micron silicon dioxide obviously increases at dosage of 0.036 to 0.571 mg/cm2 which shows a time-effect relationship.The level of LDH leakage caused by nanosized silica is higher than that by micron silicon dioxide at same concentration.The increase of lipid peroxidation product of MDA and free radicals of H2O2,·OH,O2·-and NO of RAW264.7 cells result from nanosized silica and micron silicon dioxide.The effect of cytotoxicity caused by nanosized silica is stronger than that of micron silicon at same dose and the effect of oxidative stress may also be involved.
【Fund】: 国家重点基础研究发展计划(973计划)资助项目(2006CB705602).
【CateGory Index】: TB383.1
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