Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Military Medical Journal of Southeast China》 2014-01
Add to Favorite Get Latest Update

Application of transcription mediated amplification( TMA) technique in large scale voluntary blood donor screening

ZHANG Li-bo;XIA Xin-yi;MA Gui-ming;YAO Gen-hong;Medicial School,Nanjing University;Laboratory of Nanjing Red Cross Blood Center;Jinling Hospital,Medical School of Nanjing University;  
Objective To research and investigate the effect of applying the viral nucleic acid test( NAT) on the safety of blood transfusion in large scale. Methods Voluntary donors' blood samples were tested with TMA technique by detecting HBV DNA, HCV RNA,HIV1 RNA nucleic acid. The samples were test with ELISA at the same,by detecting HIV / HBV / HCV antigen or antibody. Results 208 viral nucleic acid positive samples were detected out of 54 744 donors' blood samples and the positive rate was 0. 38%. ELISA negative,NAT positive samples were further tested with discrimination assays. 45 samples were confirmed as HBV DNA positive, none as HCV RNA positive and 1 as HIV1 RNA positive. Conclusion TMA can efficiently shorten the window period of HIV,HBV and HCV infection in blood screening in large scale,therefore significantly decrease the potential risks of blood transfusion.
【Fund】: 国家自然科学基金(30901652)
【CateGory Index】: R446.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 CHENG Jun1,SUN Cang-gui 1,DAI Yu-zhu1,ZHANG Wei-qiang 2,SUN Guan-zhong 1,LI Xiao-jun 3 (1.Clinical Experimental Center,the 117th Hospital of PLA,Hangzhou 310013,Zhejiang,China;2.Field Armies'Blood Station,the 117th Hospital of PLA,Hangzhou 310013,Zhejiang,China;3.General Hospital of Nanjing Military Command,Nanjing 210002,Jiangsu,China);HBV infection status and epidemiological investigation of low-level HBsAg in soldiers of PLA stationed in Zhejiang[J];Military Medical Journal of Southeast China;2009-04
2 LIN Xiu-rong,CHEN Qiao-hui,LIN Hai.Out-patient Department,Fuzhou General Hospital of Nanjing Milirary Command,Fuzhou,Fujian 350025,China;Study on rapid detecting and genotyping of HCV gene by TaqMan-MGB probes[J];Military Medical Journal of Southeast China;2012-01
3 CHEN Yong,HU Yu-an,YUAN Da-li,YUAN Jing-wen.Nanjing University School of Medicine,Istitute of Medical Laboratory Sciences of PLA,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,Jiangsu 210002,China;Detection of HBV large surface protein and its clinical significance in patients with hepatitis B[J];Military Medical Journal of Southeast China;2012-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Xia (Blood Center Station of Huaihua City,Huaihua,Hu′nan 418000,China);Infection status of HIV in blood donors without repayment in Huaihua Hu′nan[J];International Journal of Laboratory Medicine;2012-09
2 CHEN Yong,HU Yu-an,YUAN Da-li,YUAN Jing-wen.Nanjing University School of Medicine,Istitute of Medical Laboratory Sciences of PLA,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,Jiangsu 210002,China;Detection of HBV large surface protein and its clinical significance in patients with hepatitis B[J];Military Medical Journal of Southeast China;2012-06
3 NI Hongying,OU Shanhai,XIE Jinzhen,CHEN Changrong.Xiamen Blood Center,Xiamen 361004,China;Application of single specimen nucleic acid test in donor blood screening[J];Chinese Journal of Blood Transfusion;2012-12
4 Wu Gongxiao;Lai Fuchun;Shi Dongmei;Zhang Weifang;Wu Rong;Kuang Jieping;The Blood Testing Department of Zhuhai Centre Blood Bank;;Analysis of the nucleic acid amplification technique applied in the screening of donated blood[J];International Journal of Laboratory Medicine;2013-19
5 Xiao Jiawang;Lianyungang East Hospital;;Influence of Different Type of Anesthesia(regional vs general) on Serum IL-23,IL-32 and PCT Levels of Clinical Analysis[J];Journal of Radioimmunology;2013-05
6 ZHENG Yourong;LIANG Haojian;LI Zhongping;DU Rongsong;HUANG Zhijian;LIN Yashi;LAN Lanyin;WANG Hao;HUANG Aiqiong;FU Yong-shui;Guangzhou Blood Center;;Application of nucleic acid test for the blood donors' blood screening in Guangzhou,China[J];Chinese Journal of Blood Transfusion;2013-12
7 ZENG Jingfeng;ZHENG Xin;XIONG Wen;CHEN Yunlong;ZENG Xuezhen;ShenZhen blood center;;Study on the HBV DNA presence from HBsAg-/NAT+/HBV DNA-donors screened by nucleic acid test[J];Chinese Journal of Blood Transfusion;2013-12
8 TAN Wei-guo;CHEN Wen-qi;LV Hen;LIU Yu;WANG Ping;CHEN Feng-juan;WANG Chang-jun;ZHANG Jin-hai;Center for Disease Control and Prevention of Nanjing Military Command;Nanjing First Hospital Affiliated to Nanjing Medical University;;Development of a duplex real-time PCR assay for detection of bacillus anthracis[J];Military Medical Journal of Southeast China;2013-06
9 FANG Chang-zhi1,2,FU Ying-yuan1,QIAN Rong2,XIONG Li-hong2(1.Department of Immunology,College of Basic Medicine,Nanchang University, Nanchang 330006,China;2.Clinical Laboratory,Jiangxi Province Blood Centre, Nanchang 330077,China);Residual Risk of Transfusion-transmitted HBV Infection in HBsAg-negative Blood Donors[J];Journal of Nanchang University(Medical Science);2012-12
10 ZHANG Yan;WANG Xin;CAO Dan;LI Wen-chao;YAN Xin;ZHOU Yuan;Shandong Blood Center;;Analysis of blood test results among blood donors in Jinan from 2010 to 2012[J];Journal of Community Medicine;2013-22
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 YAO Feng-lan, REN Fu-rong, WANG Zhuo-yan,et al (Blood Transfusion Safety Research Section, Beijing Red Cross Blood Center, Beijing 100088);Improvement of the discriminative rate of the unresolved nucleic acid testing reactive samples with viruses concentrated by centrifugation[J];Beijing Medical Journal;2009-11
2 Wang Xun,Zheng Lan,Zhang Xi,et al. Shanghai Blood Center, Shanghai 200051, China;Preliminary experiences of nucleic acid testing in blood screening in Shanghai[J];Chinese Journal of Blood Transfusion;2003-03
3 REN Furong1,WANG Jingxing2,ZHAO Haiyan1,NESS Paul3,SHAN Hua3,et al.1.Beijing Red Cross Blood Center,Beijing 10088,China;2.Institute of Blood Transfusion,Chinese Academy of Medical Sciences;3.Johns Hopkins Medical Institution,USA;Nucleic acid testing of human immunodeficiency virus and hepatitis C virus among blood donors from five cities of China: estimation of residual transfusion risk[J];Chinese Journal of Blood Transfusion;2007-06
4 WU Yuqing,YANG Zhongsi,ZHAO Lin,et al.Qingdao Blood Center,Qingdao 266071,China;Study of nuclei-acid-test(NAT) among volunteer blood donors from Qingdao area[J];Chinese Journal of Blood Transfusion;2008-11
5 YE Xian-lin,LI Huo,XU Xiaoxuan,et al.Shenzhen Blood Center,Shenzhen,Guangdong 518035,China;Application on nucleic acid amplification technigues in blood screening for HBV DNA,HCV RNA/HIV-1 RNA[J];Chinese Journal of Blood Transfusion;2010-01
6 YAO Ren-nan1,Zhang Jian-hui2,Chen Fu-xin1,HUANG Xiao-jing1,ZHAO Qin1,JIANG Xue-cheng1 (1.The 97th Hospital of PLA,Xuzhou 221004,Jiangsu,China;2.Hunan Jingda Gene Co.LTD.,Changsha 410000,Hunan,China);Preliminary application of hepatitis C virus core antigen detection kit[J];Military Medical Journal of Southeast China;2008-03
7 CHENG Jun1,SUN Cang-gui 1,DAI Yu-zhu1,ZHANG Wei-qiang 2,SUN Guan-zhong 1,LI Xiao-jun 3 (1.Clinical Experimental Center,the 117th Hospital of PLA,Hangzhou 310013,Zhejiang,China;2.Field Armies'Blood Station,the 117th Hospital of PLA,Hangzhou 310013,Zhejiang,China;3.General Hospital of Nanjing Military Command,Nanjing 210002,Jiangsu,China);HBV infection status and epidemiological investigation of low-level HBsAg in soldiers of PLA stationed in Zhejiang[J];Military Medical Journal of Southeast China;2009-04
8 ZEN Jin-feng,LI Huo,XU Xiao-xun,et al. (The Clinical Laboratory of Shenzhen Blood Centre 518035,China);Analysis of Positive Blood Donors from NAT Screening:Comparison with ELISA Tests[J];International Journal of Laboratory Medicine;2010-06
9 XIE Yunzheng,CHEN Lingyan,LI Chao,ZHENG Lan,ZHOU Guoping.Shanghai Blood Center,Shanghai 200051,China;Detection of HBV DNA in non-remunerated blood donors in Shanghai[J];Chinese Journal of Blood Transfusion;2012-08
10 LEI Jian-hua~, YANG Xu, LUO Hong-yu, WANG Wen-long, HUANG Li (Liver Disease Research Center, Second Xiangya Hospital, Central South University, Changsha 410011,China);Serum HBsAg concentration and HBV replication level in hepatitis B patients with positive serum HBsAg and HBeAg[J];Journal of Central South University(Medical Sciences);2006-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved