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《Acta Botanica Boreali-occidentalia Sinica》 1990-02
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CAT CYTOLOGICAL OBSERVATION ON CALLUS PRODUCTION AND EMBRYOGENESIS OF Gossypium hirsutum L.

Wang Zhe-zhi, Zhang Da-li, Li Ke-qin, Guo De-zhi and Hao Lian-fang (Department of Biology, Shaanxi Normal University, Xian 710062)  
Five Chinese cultivars of GossyPium hirsutum L.were cultured and observed.Two kinds of calli formed in all cultivars when the hypocotyls were cultured on the MS or LS medium supplemened with IAA 2mg/l and KT 1mg/L. One is fragile and light green or grey colored, while the other is hard and white or dark green colored. The former grew rapidly and eventually developed into embryogenic callus and produced embryoids. On the contrary, the latter grew solowly and never formed embryoid at al. The cytological observation and longitudinal section of the explant cultured shows that the former is developed from the cortex tissue, while the latter is from the stele tissue. It is confirmed this result through the cortex tissue and stele tissue were excised from the hypocotyl and cultured seperately. The process of embryogenesis and somatic embryo development, was described which was similar to that of zygotic embryo.
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