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Sequence and functional analyses of the tapetum-specific promoter Osg6B from rice (Oryza sativa L.)

ZHANG Xiao guo 2,LIU Yu le 1,YANG Qing ping 2,TIAN bo 1 (1 Institute of Microbology,Chinese Academy of Science,Beijing 100080,China;2 College of Life Science,Wuhan University,Wuhan 430072,China)  
The tapetum specific promoter ( Osg 6B) from rice was sequenced artificially.The results indicated that the eight nucleotides in the cloned 1.73 kb Osg 6B was different from that the reported.They shared a 99.5% sequence homology.The cloned promoter was fused with the GUS cassette.The resultant construct was introduced into the anther and leaf of tobacco by particle bombardment.The GUS activity in more than two mm anthers was eight times that from leaves and its negative control,and in equal to or less than two mm anthers it was twelve times.These results showed that the cloned Osg 6B promoter performed its function promoting alien gene specitic expressions in plant anthers.
【Fund】: 国家863项目;; 武汉市晨光计划资助
【CateGory Index】: Q946-33
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