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《Acta Botanica Boreali-occidentalia Sinica》 2001-05
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RAPD analysis for mitochondrial DNAs of the BT cytoplasmic male sterile (cms) line and its associated lines in rice (Oryza sativa)

YAO Fang-yin1,LI Guang-xian1,ZHU Chang-xiang2,WEN Fu-jiang2 (1 Shandong Rice Research Institute,Jining,Shandong 272077,China;2 College of Life Sciences,Shandong Agricultural University,Taian,Shandong 271018,China)  
BT type rice cytoplasmic male sterility line were compared with its maintainer,restorer and F 1 hybrid using mitochondrial DNA (mtDNA) RAPD analysis.The results showed that difference was between not only the cms line and its maintainer,but also cms line and its F 1 hybrid.Primer OPJ-08 generated a polymorphic product of 800 bp in sterile line,and primer OPK-10 amplified a specific fragment of 900 bp in F 1 hybrid.The two fragments were then cloned into plasmid vector and converted to RFLP probes.Southern hybridization patterns revealed polymorphism between sterile line and F 1 hybrid using OPJ-08 800 fragment as probe,and between sterile line and maintainer line using OPK-10 900 fragment as probe.We infered that the two mtDNA fragments may be associated with cms in rice.
【Fund】: 山东省自然科学基金资助项目 (Y98D1 0 0 5 8)
【CateGory Index】: S511.01
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