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《Acta Botanica Boreali-occidentalia Sinica》 2001-05
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The application of RAPD technology in the genetic classification of Amaranthus

CHEN Jing-tang1,WAYOU Nemoto2,LIU Zhi-Zeng1, CHI Shu-Min1,MINEO Minami2,AKIO Ujihara2 (1 Agricultural University of Hebei,Baoding,Hebei 071001 China;2 Faculty of Agriculture Shinshu University,Minamiminowa Nagano 399-4598,Japan)  
Fourteen systems of seven Amaranthus L.species derived from Asian and Middle-South America were classified based on the RAPD data in this study.With eight effective primers selected from 80 ten-base random primers,103 DNA fragments were amplified,or average 12.9 DNA fragments amplified by each primer.Among all of the amplified DNA fragments,95 fragments or 92.2 percent were polymorphic.UPGMA (Unweighted pair group method using an arithmetic average)classification of the tested materials was conducted according to DNA fragments amplified with eight effective primers.The results shown that all of the materials could be divided into the four following groups:(1) include two systems of weed species,A.deflexus and A.dubius;(2) ancestor wild species A.hybridus;(3)two systems of seed-use cultivated species,i.e.six of A.hypochondriacus and two of A.caudatus;(4) include two systems of A.tricolor and one of A.mangostanus,all belonged to the vegetable species.
【CateGory Index】: Q949.745.2
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