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《Acta Botanica Boreali-occidentalia Sinica》 2002-05
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Construction of high-level expression vectors containing sgna gene for use in wheat Transformation

HOU Wen sheng 1,GUO San dui 2,LU Ming 1(1 Yangling Branch of China Wheat Improvement Center,College of Agronomy,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Biotechnology Research Institute,Chinese Academ  
In the report,the maize ubiquitin 1 promoter Ubi ,a high efficiency promoter in gramineous crop,had been introduced into the original plasmid with s gna gene.Expression box of bar gene had been also inserted as a selectable marker.In order to improve the expression level of the target genes,Ω kozak sequence and poly (A) sequence were added to the 5′ end and 3′ end of the target genes,respectively.The wheat expression vectors with insect resisting gene pGU4AGBar and pGBIU4AGBar had been constructed,respectively.pGU4AGBar contains isodirectional expression box of Ubi s gna and Ubi bar .pGBIU4AGBar contains T DNA boundary sequence,and isodirectional expression box of CaMV35S nptⅡ,Ubi s gna and Ubi bar had been inserted within the T DNA boundary sequence.The sgna gene,a synthetical agglutinin gene of Galanthus navies,codes a poisonous protein for Homoptera .
【Fund】: “九五”国家重点科技攻关资助项目 (专题 ) (96-0 0 9-0 1 -1 4);; 杨凌农业生物技术育种中心开放课题资助项目 (1 999-1 9)
【CateGory Index】: Q943.2
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