Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Botanica Boreali-occidentalia Sinica》 2003-09
Add to Favorite Get Latest Update

Cytological observation and analysis of isoenzyme patterns in three types of hairy roots transfromed by Agrobacterium rhizogenes A_4

ZHANG Gaina,JIA Jingfen*,HAO Jianguo,CHEN Gang(School of Life Science, Northwest University Xi'an 710069,China)  
The chromosome numbers were checked using squash method and the isoenzyme patterns of Peroxidase(POD)、Cytochrome oxidase(COD) and Esterase(EST) were analyzed by means of polyacrylamide gel electrophoresis in three different types of Agrobacterium rhizogenes A4transformed hairy roots. The results showed that: (1) Chromosome lose existed commonly in every type of hairy roots. The percentage of chromosome lose in the nonregenerable lines was obviously more than that in the regenerable ones. (2) More obvious changes of isoenzyme patterns of POD and COD were observed in the nonregenerable line compared with that of regenerable lines. The content of EST was rather weak in nonregenerable line.
【Fund】： 国家自然科学基金项目(No.30070366 )
【CateGory Index】： Q942.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 LIU Ming-Zhi, CHEN Qin, LIU Xi( Department of Bioengineering and Enviromental Science, Changsha University, Changsha 410003, China );Changes of superoxide dismutase and estrase in processes of embryogenic calli induction of Eustoma grandiflorum[J];Guihaia;2008-02
【Citations】
Chinese Journal Full-text Database 4 Hits
1 WANG Yu mei 1,LI Wu xing 2,CHEN Xi 2,JIA Jing fen 3* (1 Shanghai Institute of Plant Physiology,The Chinese of Sciences,Shanghai 200032,China;2 Cell Biology Laboratory,Lanzhou University,Lanzhou 730000,China;3 Biotechnology Institute,Northwest;Studies on the isozymes of transformaed calli of Linum usitatissimun,Onobrychis viciaefolia Scop.and Alhagi pseudalhagi obtained by Agrobacterium tumefaciens mediated chitinase gene transformation[J];Acta Botanica Boreali-occidentalia Sinica;2001-01
2 Wamg Yumei1 ,Wang Minggang2 ,Wang Jiangbo1 ,Jia Jingfen1 ( 1.College of Life Science,L anzhou University,L anzhou,730 0 0 0 ,China; 2 .Department of Biology,Xiamen U niversity,Xiamen,3610 0 5 ,China);Genetic Transformation of Alhagi Preudoalhagi with Agrobacterium rhizogenes A4[J];Journal of Lanzhou University;2000-01
3 Xu Ziqin\ Jia Jingfen\ Hu Zhide * (Cell Biology Laboratory of Biology Department and Chemistry * Department of Lanzhou University, Lanzhou 730000);Transformation of Alfalfa Suspension Cultures by Agrobacterium rhizogenes[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1997-01
4 LIU Chuan_Fei, YU Shu_Hong, LI Ling *, SHI He_Ping, PAN Rui_Chi [WT6BZ](Department of Biology, South China Normal University, Guangzhou 510631, China);The Genetic Transformation of Pueraria as Medicinal Plant by Agrobacterium rhizogenes[J];ACTA BOTANICA SINICA;2000-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Li Qing \ Su Xuehen \ Li Zhandong (College of Landscape Architecture, Beijing For.Univ.,100083,P.R.China)\;;Rapid Propagation of Climbing Rose \%in vitro\%[J];Journal of Beijing Forestry University;1999-06
2 Tang Wei & Wu Jiangyun(Northeast Agricultural College, Harbin 150030,China);STUDIES ON CHROMOSOME VARIATION IN CALLUS SUBCULTURE OF FRITILLARIA USSURIENSIS MAXIM[J];JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY;1994-01
3 Xu Tiefeng, Zhang Hanming, Ding Ruxian, Li Bohua, Zhang Qiaoyan, Zheng Hanchen (Department of Pharmacognosy, College of Pharmacy, Second Military Medical University, Shanghai,200433);Study on hairy roots of Mollugo pentaphylla L.[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1999-10
4 XU Tie Feng 1, ZHANG Han Ming 1, ZHANG Wei 2, DING Ru Xian 1, LI Bo Hua 3 (1.Department of Pharmacognosy, College of Pharmacy, Second Military Medical University, Shanghai 200433, China; 2.Department of Physiology, Harbin Medical University ;;Establishing better lines and regenerating system of hairy roots of Isatis indigotica with Ri T-DNA[J];Academic Journal of Second Military Medical University;2000-10
5 BU Huai yu,JING Jian zhou,HAO Jian guo,JIA Jing fen * (Department of Biology,Northwest University,Xian 710069,China);Effect of different factors on transformation of Alhagi pseudalhagi by Agrobacterium rhizogenes[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;2000-04
6 LI Shiweng1,FAN Xiaofeng2,TIAN Xingwang2,WANG Yimin2 (1 School of Chemical Engineering,Lanzhou Jiaotong University,Lanzhou 730000,China;2 Department of Biology,Qingyang Teachers College,Xifeng,Gansu 745000,China);Studies on tissue culture and micropropogation of Bougainvillea glabra[J];Acta Botanica Boreali-occidentalia Sinica;2003-06
7 LI Qun~1, LIU Guang-yong~2, WANG Li~(3*)( 1.College of Chemistry and Life Science, Sichuan Normal University, Chengdu 610066, China; 2.Chengdu Mengzhuiwan Middle School, Chengdu 610051, China; 3.College of Life Science, Sichuan University, Chengdu 610064, China );Effect of hormone on plantlet regeneration and studies of root regeneration culture of Eustoma sp.[J];Guihaia;2004-01
8 WANG Yu-chun; LIU Chun-zhaos; ZHAO Bing;OUYANG Fan; YE He-chun; LI Guo-feng(State Key Lab. BioChem. Eng., Inst. Chem. Metall., CAS, Beijing 100080, China)(Inst. Botany) Chinese Academy of Sciences, Beijing 100093, China);Kinetics and Stoichiometry in Suspension Culture of Artemisia annua L Hairy Root[J];ENGINEERING CHEMISTRY & METALLURGY;2000-01
9 Zhao Jie;Cheng Jinchen(Department of Biology,Central China Normal University,Wuhan);STUDIES ON THREE KINDS OF ISOZYMES CHANGS DURING DEDIFFERENTIATION OF THE SHOOT SECTION IN ASPARAGUS OFFICINALIS L.[J];JOURNAL OF CENTRAL CHINA NORMAL UNIVERSITY(NATURAL SCIENCES);1994-03
10 Zhang Qian Zheng Guochang (Laboratory of Cell Biology, Lanzhou University, Lanzhou730000);Study on Chromosome Variation in Callus and Regenerated Buds of Onobry Viciaefolia Scop. under Long term Culture[J];Journal of Lanzhou University;1996-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 HUANG Hai-quan1,CHEN Xiao-hua1,LIU Qi-yuan2,HUANG Meijuan 1(1.College of Landscape Architecture,Southwest Forestry College,Kunming 650224,China;2.College of Agronomy,JAU,Nanchang 330045,China);Studies on Tissue Culture and Rapid Propagation of Altemanthera ficiode cv.'Ruliginosa'[J];Acta Agriculturae Universitatis Jiangxiensis;2008-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Jinyuan, Shi Xiangyu, Chen Xiao, Zeng Zhaohai,Zhou Zhuhua & Chen Jianai(Institute of Atomic Energy Application in Agriculture,Shandong Acodemy of Agricultural Sciences,Jinan 250100);Study on Transformation of Wheat and Maize Via Gene-gun Bombardment[J];SHANDONG AGRICULTURAL SCIENCES;1999-02
2 Zhang Ghenghe (Dept.of Hort., Hebei Agri.Univ., Baoding, China);Gene Transfer in Higher Plants and Its Potency in Crop Improvement[J];Journal of Agricultural University of Hebei;1991-04
3 Ni Jianfu and Zhou Wenlin(Institute of Cereal Crops, Gansu Academy of Agricultural Sciences,Lanzhou 730070)Niu Dengke;WangMinggang and Wang Yafu(Department of Biology, Lanzhou University,Lanzhou730000);STUDIES ON GLIADINS OF VARIETIES OF THE WHEAT PROGENTES OBTAINED BY INTRODUCING DNA OF AGROPYRON ELONGATUM[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1995-05
4 Gong Zhenghui(Department of Horticulturen, Northwester Agricultural University, Yangling Shaanxi 712100 ) Milner Joel Joseph(Department of Botany,University of Glasgow,U. K. )He Yuke(Shanghai Institute of Plant Physiolology,Academia Sinica,Shanghai 200032;STUDIES ON A NEW GENE TRANSFORMATION METHOD:VACUUM INFILTRATION TRANSFORMATION[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1996-03
5 Wang Guangqing; Wang Yunzhu; Hu Rongxia; Yang Jinshui; Ge Koulin(Institute of Genetics)Li Meizhen; Chen Genfa; Zheng Zhenjun; Wang Yinxue; Wang Chuiding(Pengpu, Zhabei District);Preliminary study of the introduction of foreign DNAs into cabbage(Brassica chinensis L.) via pollen-tube pathway[J];;1996-02
6 Zhang Liping;Cao Ziyi;Li Wei Department of Agronomy, Gansu Agricultural University;Progress in Plant Transgenic Techniques[J];Journal of Gansu Agricultural University;1991-03
7 Ma Bojun ( Department of Biology, Zhejiang Normal University , Jinhua 321004)Yuan Miaobao ( College of Life Science, Hangzhou University , Hangzhou 310012)Wang Guanqing Yang Jinshui Ge Koulin ( Institute of Genetics, Fudan University , Shangh;A convenient foreign gene transfer system in barley[J];GUIHAIA;1998-01
8 Yan Xinfu;Liu Wenxuan(Wheat Institute, Hanan Academy of Agricultural Sciences, Zhengzhou 450002)Lin Qiuping(Laboratory Centre,Henan Academy of Agricultural Sciences, Zhengzhou 450002);A Study on the Esterase Isoenzyme of the Variants Derived from Barley DNA Introduction into Common Wheat[J];ACTA AGRICULTURAE BOREALL—SINICA;1994-01
9 Fu Guirong; Xu Zhiru; Zheng Baojiang;Huang Yongfen;Wang Qingyin; Li Hongquan(Harbin Normal University);STUDIES ON TRANSFORMATION OF SEED-GERMINATING EMBRYOES OF SOYBEAN BY A.TUMEFACIENS MEDIATED IPT GENE ⅡAnalysis of the Isozymes of the Seedling Stage Roots[J];NATURAL SCIENCE JOURNAL OF HARBIN NORMAL UNIVERSITY;1996-04
10 Wan Wenju;Zou Dongsheng, (Department of Agronomy, HNAC, Changsha, 410128) Peng keqin, (Department of Basic Sciences, HNAC, Changsha, 410128);ON BIOMUTAGEN-INDUCED MUTATION——DOUBLE FUNCTION OF EXOGENOUS DNA INTRODUCTION[J];;1992-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved