Cytological observation and analysis of isoenzyme patterns in three types of hairy roots transfromed by Agrobacterium rhizogenes A_4
ZHANG Gaina,JIA Jingfen*,HAO Jianguo,CHEN Gang(School of Life Science, Northwest University Xi'an 710069,China)
The chromosome numbers were checked using squash method and the isoenzyme patterns of Peroxidase(POD)、Cytochrome oxidase(COD) and Esterase(EST) were analyzed by means of polyacrylamide gel electrophoresis in three different types of Agrobacterium rhizogenes A4transformed hairy roots. The results showed that: (1) Chromosome lose existed commonly in every type of hairy roots. The percentage of chromosome lose in the nonregenerable lines was obviously more than that in the regenerable ones. (2) More obvious changes of isoenzyme patterns of POD and COD were observed in the nonregenerable line compared with that of regenerable lines. The content of EST was rather weak in nonregenerable line.