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《Acta Botanica Boreali-occidentalia Sinica》 2003-09
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Protoplast preparation and induced screening of alkaline pullulanase producing bacterium

MA Xiaojun1,CHEN Tuo2,ZHANG Gaosen1,ZHANG Xiaojun2,WANG Rui1(1 School of Life Science, Lanzhou University, Lanzhou 730000,China;2 Laboratory of Ice Core and Cold Region Environment, Cold and Arid Region Environmental and Engineering Research Institute, CAS, Lanzhou 730000,China)  
The optimal condition for preparating of the protoplast and its regenerating were studied. Under 0.5 mg/mL of lysozyme,the preparation ratio reach to 93.8% when cells were lysed at 37℃ for 1.5 h.The optimum steadyreagent of protoplast regeneration is mannitol.The regeneration ratio is above 26.4%. Protoplast of Bacillus sp SX12 were induced by ultraviolet ray. One high producing strain (SX12 C67) were obtained from process of screening. The activity of pullulanase in strain SX12 C67 increased from 2.42 μ/mL in strain SX12 to 6.87 uμ/mL, about 1.8 times than strain SX12.
【Fund】: 中国科学院寒区旱区环境与工程研究所冰芯室创新项目资助(210506 )
【CateGory Index】: Q939.9
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