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《Acta Botanica Boreali-occidentalia Sinica》 2005-03
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Occurrence and Control of Abnormal Somatic Embroys in Saposhnikovia divaricata Tissue Culture

MA Ji,QIAO Qi,XIAO Ya-ping,WANG Pei-xiang,WANG Zhe-zhi (College of Life Science,Shaanxi Normal University,Xi'an 710062,China)  
The effect of various factors on the occurrence and growth of abnormal somatic embryos were studied.Tissue culture and paraffin section were adopted.The efficient medium combination to induce somatic embryos can be concluded as the induction medium of embryonic callus is MS+0.5 mg/L 2,4-D,the concentration of sucrose is 3%;the differentiation medium is MS+1 mg/L 6-BA +0.2 mg/L NAA+0.5 mg/L ABA,the concentration of sucrose is 4%~5%;the maturation medium is MS,the concentration of sucrose is 3%.A certain concentration of auxin is the key factor to induce embryonic callus and cytokinin is cooperative in the differentiation and growth of somatic embryos.The concentration of sucrose,ABA,inacubition method and appropriate culture condition container can reduce the rate of abnormal somatic embryos.
【Fund】: 国家 十五 攻关基金资助项目(77114 8);; 陕西师范大学优秀研究生基金项目
【CateGory Index】: S567.2
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