Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Botanica Boreali-occidentalia Sinica》 2005-03
Add to Favorite Get Latest Update

Occurrence and Control of Abnormal Somatic Embroys in Saposhnikovia divaricata Tissue Culture

MA Ji,QIAO Qi,XIAO Ya-ping,WANG Pei-xiang,WANG Zhe-zhi (College of Life Science,Shaanxi Normal University,Xi'an 710062,China)  
The effect of various factors on the occurrence and growth of abnormal somatic embryos were studied.Tissue culture and paraffin section were adopted.The efficient medium combination to induce somatic embryos can be concluded as the induction medium of embryonic callus is MS+0.5 mg/L 2,4-D,the concentration of sucrose is 3%;the differentiation medium is MS+1 mg/L 6-BA +0.2 mg/L NAA+0.5 mg/L ABA,the concentration of sucrose is 4%~5%;the maturation medium is MS,the concentration of sucrose is 3%.A certain concentration of auxin is the key factor to induce embryonic callus and cytokinin is cooperative in the differentiation and growth of somatic embryos.The concentration of sucrose,ABA,inacubition method and appropriate culture condition container can reduce the rate of abnormal somatic embryos.
【Fund】: 国家 十五 攻关基金资助项目(77114 8);; 陕西师范大学优秀研究生基金项目
【CateGory Index】: S567.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 9 Hits
1 XU Wen-yi et al(College of Life Science,Liaoning Normal University,Dalian,Liaoning 116021);Study on the Tissue Culture and Rapid Propagation of Oenanthe javanica(Blume) DC.[J];Journal of Anhui Agricultural Sciences;2008-14
2 FENG Da-ling1,MENG Qing-rong2,LI Wen-ping2,HU Yong-hong2,LI Ming1,GU Ai-xia3 1 College of Life Science,Agricultural University of Hebei,Baoding 071001,P.R.China 2 Baoding Research Institute of Agricultural Science,Baoding 071001,P.R.China 3 College of Landscape Architecture and Tourism,Agricultural University of Hebei,Baoding 071001,P.R.China;Morphology of somatic embryogenesis and plantlet formation in tissue cultures of lantern tree (Koelreuteria bipinnata var.integrifoliola)[J];中国林学(英文版);2010-01
3 Li Sen Lin Bochao Lv Ping Tong Shaoming Jiang Changyang(Liaoning Normal University);TISSUE CULTURE AND ESTABLISHMENT OF ASEXUAL LINE FOR GLEHNIA LITTORALIS(A.GRAY)FR.SCHMIDT EX MIQ.[J];Natural Science Journal of Harbin Normal University;2008-05
4 ZOU Cui-xia, ZHANG Shu-guang, CHEN Jia-yuan, BAO Ya-lian, JIANG Chang-yang (College of Life Science, Liaoning Normal University, Dalian 116029, China);Establishment of Regeneration Clone of Anthriscus Cereifolium[J];Journal of Liaoning University(Natural Sciences Edition);2006-03
5 YOU Cui-Rong1,2,FAN Ting-Jun1,QU Fu-Ning2,BIAN Fu-Hua2,LIANG Li-Kun2,GONG Xue-Qin2(1.College of Marine Life Science,Division of Life Science and Technology,Ocean University of China,Qingdao 266003,China;2.Scientific and Technological College of Chemistry and Biology,Yantai 264005,China);Somatic embryogenesis and study on histology and cytology in Cyclamen persicum Mill[J];Journal of Sichuan University(Natural Science Edition);2008-06
6 ZHANG Jia-jing,YU Yuan-jie~※(College of Agronomy,Shandong Agricultural University,Taian 271018,China);Research Advances in Tissue Culture of Herbal Medicine of Umbelliferae[J];Special Wild Economic Animal and Plant Research;2011-01
7 CHEN Xiao-hua,GAO Jie(College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China);Research on the Callus Culture Induction and the Secondary Culture from Cotyledon of Cuminum cyminum L.[J];Xinjiang Agricultural Sciences;2007-03
8 GAO Jie,CHEN Xiao-hua1,GAO Xiang2,SUN Jun1 (1College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China;2.Xinjiang Seed Administrition Station,Urumqi 830038,China);Research on Embryonic Callus Induction and Regenerative Plant of Hypocotyl of Cuminum cyminum L.[J];Xinjiang Agricultural Sciences;2007-06
9 KONG Dong-Mei1,2,SHEN Hai-Long3 1School of Life Science and Technology,Shanxi University,Taiyuan 030006,China;2College of Life Science,3College of Forestry,Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin 150040,China;Formation and Control of Abnormal Somatic Embryogenesis in Plant Tissue Culture[J];Plant Physiology Communications;2008-05
Chinese Journal Full-text Database 2 Hits
1 TAO Ming (Shnxi Normal University,Xian 710062,China);Studies on the abnormal embryoid and hypeahydric shoot in tissue culture[J];Acta Botanica Boreali-occidentalia Sinica;2001-05
2 1,2 CHENG Yu_Lan 2DIAO Feng_Qiu 2WU Nai_Hu 1HUANG Mei_Juan  (College of Life Sciences, Peking University, Beijing 100871) (Institute of Developmental Biology, The Chinese Academy of Sciences, Beijing 100080);Effects of Sucrose Regulation Culture on Endogenous ABA Levels of Carrot Somatic Embryo [J];ACTA BOTANICA SINICA;1999-07
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yuan-guo~1,LUN Jun~2,WANG Zhen-hua~3,ZHANG Liquan~1,LI Fang~1(1.Weifang Academy of Agricultural Sciences,Weifang 261061,China;2.Weifang Gardens Bureau,Weifang 261041,China;3.Weifang Professional College,Weifang 261041,China);Investigation and Preliminary Microscopic Examination on the Andromonoecious Plants of the Asparagus[J];Shandong Agricultural Sciences;2006-04
2 CAO Tian-Xu et al(Department of Horticulture,Agriculture College of Yanbian University,Longjing,Jilin 133400);Analysis of the Formal Shoot and Vitrification Shoot from Gypsophila Paniculata L Tissue Culture with the RAPD[J];Journal of Anhui Agricultural Sciences;2007-03
3 XU Chun-ming et al(Beijing Key Laboratory of Plant Resources Research and Development,College of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100037);Activity Change of Antioxidase System in Bud Differentiation Process of Saussurea involucrata[J];Journal of Anhui Agricultural Sciences;2009-09
4 HAN Lu,LIU Bi-rong,PAN Chao,ZHANG Xiao-ping~* (College of Life Science, Anhui Normal University, Wuhu 241000, China);TISSUE CULTURE AND RAPID PROPAGATIONP OF VETIVERIA ZIIZANIOIDES[J];Journal of Anhui Normal University(Natural Science);2004-04
5 Shi xuehui Yang Mengling Zhang Qiuming Chen Aihua Wen Qiongying Sun Hui Luo Chuanhui Department of Horticulture, HNAU, Changsha, 410128) Liu Sha He Toun, Ning Xiang County, Hunan Prorince) Hunan City Construction School, Zhuzhou, Hunan Skill;Study on Tissue Culture of Xiang yang Pear[J];AMINO ACIDS & BIOTIC RESOURCES;1997-01
6 Jia Rong Xie Huian Cheng Kai Qiu Weiming (Biology Department,Anhui University,Hefei 230039);Effects of Some Kinds of Albumen on the Germination of Isolated Wheat Embryo[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1998-01
7 PATIMAN·Abdurahiman,QIN Yong,YUSUFU·Ablitifu(College of Horticulture,Xinjiang Agricultural University,Urumqi,Xinjiang 830052,China);Study on Tissue Culture of Foeniculum vulgare Mill. Fresh Stem[J];Northern Horticulture;2007-12
8 YANG Li-qin,LI Rui,WANG Jun,HU Hai-ying,WU Xiao-ling(Ningxia University,Ningxia,Yinchuan 750021,China);Three Difficult Problems in Plant Tissue Culture[J];Northern Horticulture;2008-04
9 Li Hui;Chen Xiaoyang;Li Yun;Li Wei;Guo Hai.School of Biological Sciences and Biotechnology, Beijing For. Univ., 100083, P. R. China.;Establishment of high frequency regeneration system of adventitious bud from leaves of Populus alba[J];Journal of Beijing Forestry University;2004-03
10 Zhang Qinying Chen Jun-yu (College of Landscape Architecture , Beijing Forestry University, 100083,P.R.China) Shen Zuolian (College of Agronomy and Biotechnology, China Agricultural University,100094,Beijing,P.R.China);Influences of Growth Regulators on in vitro Culture of Leaves of Mei (Prunus mume `Meiren') and Cytological Observation[J];Journal of Beijing Forestry University;2004-S1
China Proceedings of conference Full-text Database 7 Hits
1 BAI Yong-fu LU Xiu-ping (Yunnan Tobacco Research Institute, Yunnan Yuxi 653100, China);Advances in study on plant artificial seed and its application foreground on tobacco[A];[C];2006
2 Li Dongmei;Lai Zhongxiong;Guo Zhixiong;Pan Dongming Institute of Subtropical Fruits, Fujian Agriculture and Forestry University, Fuzhou 350002;The changes of the coutenty of soluble sugars in the process of somatic embryo maturation in Longan[A];[C];2003
3 Zhan Yuanfeng;Wang Guangdong;Cheng Zhiying College of Horticulture Nanjing Agrcultural University Nanjing 210095;Somatic Embryogenesis and Maturation into Normal Plants in Garlic (Allium sativum L.)[A];[C];2004
4 Chen Fadi Jiang Jiafu Guo Weiming FangWeimin Zhao Hongbo (College of Horticulture,Nanjing Agricultural University,Nanjing,210095,China);Studies on Cell Suspension Culture and Plant Regeneration of Dendranthema×grandiflorum with Small Inflorescences[A];[C];2002
5 CHEN Fa-di JIANG Jia-fu GUO Wei-ming (College of Horticulture,Nanjing Agric Univ,Nanjing 210095,China);Study on irradiation breeding of suspension cell of Dendranthema morif olium with small inflorescence Ⅰ.Selection of genotype,explant and callus induction media[A];[C];2002
6 Ouyang Jie Wei Liqiang Wu Yanzhen Yuan Dehai ( Department of Food Science and Engineering, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083) Beijing Jin- ye Bioengineering Co., Ltd., Beijing 101119);Production of Natural Flavor & Fragrance by Biotechnology[A];[C];2006
7 LIU Xue-jing~1,DAI Li~2,YIN Bao-zhong~1,ZHEN Wen-chao~(1,3) (1 College of Plant Protection,Agricultural University of Hebei,Baoding, 071001;2 College of Horticulture,Agricultural University of Hebei,Baoding, 071001;3 Research Center for Biocontrol Techniques against Pests on Crops of Hebei Province,Baoding,071001);Effects of Polyvinyl Alcohol on Tissue Culture of Strawberry[A];[C];2009
Chinese Journal Full-text Database 10 Hits
1 ZHANG Gui-hua, WANG Hong-mei, WANG Lian-xiang (Heze Institute of Agricultural Sciences, Heze 274000, China);Study on tissue culture techniques for peony[J];Shandong Agricultural Sciences;2001-05
2 TAN Xing et al(School of Life Sciences,China West Normal University,Nanchong,Sichuan 637002);Research on the Tissue Culture and Plantlet Regeneration of Heracleum candicans[J];Journal of Anhui Agricultural Sciences;2006-20
3 HU Fang-fang et al(Institute of Cellular and Molecular Biology,School of Life Science,Xuzhou Normal University,Xuzhou,Jiangsu 221116);Study on Tissue Culture of Changium smyrnioides Wolff Leaf[J];Journal of Anhui Agricultural Sciences;2007-08
4 LIANG Qiao-ling et al(College of Chemistry and Life Science,Lishui University,Lishui,Zhejiang 323000);Induction of Callus in Saccharum Sinense and the Plant Regeneration[J];Journal of Anhui Agricultural Sciences;2007-32
5 GAO Chang-yong (Department of Life Sciences,Heze University,Heze,Shandong 274015);Research of Inducing Callus with Different Peony Explants[J];Journal of Anhui Agricultural Sciences;2007-34
6 ZHANG Yan-bo et al(College of Life and Environment Sciences,Hangzhou Normal University,Hangzhou,Zhejiang 310036);Studies on the Callus Induction and Browning Inhibition of Camptotheca acumianta[J];Journal of Anhui Agricultural Sciences;2007-36
7 CHEN Hong et al(Research Institute for Fruit Resources of Karst Mountain Region of Guizhou University,Guiyang,Guizhou 550025);Study on Anther Callus Proliferation of Rose roxburghii Tratt cv.[J];Journal of Anhui Agricultural Sciences;2008-11
8 WANG Da-ping et al (Chongqing University of Arts and Sciences, Chongqing 402168);Study on Rooting Culture in Tissue Culture Seedlings of Actindia deliciosa[J];Journal of Anhui Agricultural Sciences;2008-21
9 CAO Xiao-yong (Bio-resources Key Laboratory of Shaanxi Province,Hanzhong 723001, China);In Vitro Culture of Embryo of Endangered Plant Paeonia rockii[J];Amino Acids & Biotic Resources;2003-02
10 Zheng Caixia Gao Rongfu(Forest Resources College, BFU);Effect of ABA and Other Endogenous Inhibitors on Fraxinus pennsylvanica Marsh .Seeds Dormancy[J];Journal of Beijing Forestry University;1991-04
【Secondary References】
Chinese Journal Full-text Database 8 Hits
1 SHEN Zong-Gen~1,MO Ying-Yan~1,SHEN Ying~1,ZHANG Ya-Cheng~2,XU Xu~2,WAN Hong-Wei~2 (1.School of Biology and Food Engineering,Changshu Institute of Technology,Changshu 215500,China; 2.College of Medicine,Suzhou University,Suzhou 215123,China);Studies on Tissue Culture and Germplasm Conservation Techniques of Oenanthe javanica[J];Journal of Changshu Institute of Technology;2009-04
2 YE Yuanying, KE Weidong, LIU Yimain, HUENG Xinfang ( Wuhan Vegetable ResearchInstitute, Wuhan 430065 );Structure of Vegetative organ of Oenanthe stolonifera DC.[J];Journal of Changjiang Vegetables;2009-16
3 FENG Da-ling1,MENG Qing-rong2,LI Wen-ping2,HU Yong-hong2,LI Ming1,GU Ai-xia3 1 College of Life Science,Agricultural University of Hebei,Baoding 071001,P.R.China 2 Baoding Research Institute of Agricultural Science,Baoding 071001,P.R.China 3 College of Landscape Architecture and Tourism,Agricultural University of Hebei,Baoding 071001,P.R.China;Morphology of somatic embryogenesis and plantlet formation in tissue cultures of lantern tree (Koelreuteria bipinnata var.integrifoliola)[J];中国林学(英文版);2010-01
4 LI Ying et al(Qingdao Agricultural University,Qingdao,Shandong 266109);Study on Disinfection Method of Glehnia littoralis Explants[J];Journal of Anhui Agricultural Sciences;2010-05
5 SONG Gang et al(College of Life Sciences,Nanjing Aguiculture University,Nanjing,Jiangsu 210095);Research Progress in Tissue Culture of Cyclamen persicum Mill.[J];Journal of Anhui Agricultural Sciences;2010-07
6 MA Yan-ming1,LIU Zhi-yong1,Wang Hao1,Han Wen-wen2,Reyilamu·Muhefuli1,BAI Yu-ting1 (1.Institute of Crop Germplasm Resource,Xinjiang Academy of Agricultual Sciences,Urumqi 830091,China;2.College of Agronomy,Xinjiang Agricutural University,Urumqi 830052,China);Analysis on ISSR Markers of Germplasm for Cuminum cyminum L.in Xinjiang[J];Xinjiang Agricultural Sciences;2008-01
7 LI Nan1,2,KONG Dong-Mei2,3,SHEN Hai-Long1,2,1School of Forestry,Northeast Forestry University,Harbin 150040,China; 2Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin 150040,China; 3College of Life Science and Technology,Shanxi University,Taiyuan,Shanxi 030006,China;Effect of Low Temperature Pretreatment on Somatic Embryogenesis of Fraxinus mandshurica Rupr.[J];Plant Physiology Communications;2009-06
8 Cai Xiaodong,Zhang Jinjing,Lin Lijun (College of Horticulture and Gardening,Yangtze University,Jingzhou Hubei 434025);Studies on physiological and biochemical characters of malformed somatic embryoids ofCitrus Sinensis L.Osbeck cv.Bingtang[J];Chinese Agricultural Science Bulletin;2010-20
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Yun;Tian Yanting;Luo Xiaofang(Research Centre of Improved Cultivar Breeding, BFU 100083);A Preliminary Study on the Mechanism of Vitrification of Mains zumi[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1996-01
2 Hao Guixia Zhu Zhen (Institute of Genetics, Chinese Academy of Sciences, Beijing, 100101, P. R. China) Zhu Zhiti (College of Forest Resources and Environment, Beijing For. Univ., 100083, P. R. China);Vitrification of Regeneration Shoots of P.tomentosa[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-01
3 Shi Xiaoxin Chen Siwei Ma Baokun Gao yi (Dept.of Hort., Hebei Agri.Univ., Beoding, China);Studies on Vitrification Occurred in Rapid Propagation of Apple Rootstock[J];Journal of Agricultural University of Hebei;1990-03
4 Li Keqin;Wang Zhezhi;Zhang Dali;Guo Dezhi;HaoLianfang;Zhang Sufeng;Sheng Zhigang, (Department of Biology, Shaanxi Normal University, Xi'an 710062);STUDIES ON TISSUE AND CELL CULTURE OF GOSSYPIUM HIRSUTUM L.[J];Acta Botanica Boreali-occidentalia Sinica;1991-02
5 Liu Quanhong, Xiao Yaping, Wang Zhezhi, Ren Yaohui,Chen Liqin, and Xue Yi(Department of Biology, Shaanxi Normal University, Xi;an 710062);STUDY ON COMPARATIVE ANATOMY OF REGENERATIVE PLANTLETS OBTAINED IN TISSUE CULTURE OF GOSSYPIUM HIRSUTUM L.[J];Acta Botanica Boreali-occidentalia Sinica;1993-03
6 Zhao Jian(Institute of Materia Medica,Chinese Academca of Medicine Science,Beijing 100050)Yang Wenjie(College of Life Science,Northeast Normal University,Changchun 130024);RELATIONSHIPS OF OXYGEN FREE RADICALS STRESS AND OCCURRENCE OF VITRIFIED SHOOTS DURING MICROPROPAGATION OF PYRUS “PINGBO NO.1” (PYRUS PYRIFOLIA CULT.VAR.PINGBO NO.1) IN VITRO[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1998-02
7 Zhang Hongsheng (Yantai Fruit Research Institule. Shandong 264004) Mu Yunguan; Xin Peigang (Shandong Agricultural University, Taian 271018);STUDY ON THE MECHANISM OF VITRIFICATION OCCURED IN APPLE STEM-TIP CULTURE[J];;1991-02
8 Chu Chengcai Li Dawet (Department of Biology);THE VITRIFICATION IN TREE PEONY[J];Journal of Henan Normal University(Natural Science);1992-01
9 Li Yun\ Tian Yanting\ Luo Xizofang (Research Centre of Improved Cultivar Breeding,Beijing Forestry University, Beijing 100083);STUDY ON DISTRIBUTION AND VARIATION OF CELLULOSE, LIGNINE AND ELEMENTS IN VITREOUS SHOOT OF Malus zumi[J];;1997-02
10 Jia Weilong(Dry-land Agricultural Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031 )Zhou Xiaomei(Department of Life Science, Shanxi University, Taiyuan 030006) Yang Lili Gao Peng(Dry-land Agricultural Research Center,;STUDIES ON PHYSIOLOGICAL AND BIOCHEMICALCHARACTERISTICS OF VEIRFIED DIANTHUSCARYOPHYLLUS SHOOTS[J];JOURNAL OF SHANXI UNIVERSITY (NATURAL SCIENCE EDITION);1997-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved