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《Acta Botanica Boreali-occidentalia Sinica》 2005-04
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Use of ITS Sequences Based on Nuclear rDNA for Phylogenetic Research of Seed Plants

LI Xue-ying,PENG Jian-ying,BAI Rui-xia (College of Horticulture, Agricultural University of Hebei, Baoding , Hebei 071 001,China)  
Nuclear rDNA in seed plants is a tandem sequence of highly-repeatable units that have become homogenized or nearly so in most species. 5. 8S rDNA makes the internal transcribed spacers of nuclear rDNA divide into ITS1 and ITS2. In angiosperms, ITS1 and ITS2 ranges between 165 bp and 298 bp, and 177 bp and 266 bp, respectively; the ITS is relatively long in gymnosperms because its length depends on ITS1 length. Direct sequencing and cloned sequencing of the PCR products of the two fragments can be done. Because ITS sequences mutate quickly, they are capable of providing many variable sites and informative sites , which have become the important molecular markers in the systematic development and classification researches of low-order angiosperm categories , and providing important systematic information for exploring polyploid reticulate evolution and allopolyploid origins, but it is not suitable for family and above-family phylogenetic researches. Because ITS in gymnosperms is relatively long, its repeated sequences differently homogenize and its sequencing is relatively difficult, it is limited to study gymnosperm phylogenetic development and classification although some advances has been made in recent years.
【Fund】: 国家自然科学基金资助项目30270927
【CateGory Index】: Q943
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