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《Acta Botanica Boreali-occidentalia Sinica》 2005-08
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Peroxidase Isozyme in Three Gastrodia elata Variants

CHEN Wen-qiang, DENG Bai-wan, DING Rui,WU San-qiao,LI Xin-sheng(Shaanxi Key Laboratory of Bio-resources, Shaanxi University of Technology,Hanzhong,Shaanxi 723001,China)  
Polyacrylamide gel electro phoresis (PAGE) was adopted to study peroxidase isozymes in three Gastrodia elata variants. The results showed that the three variants presented different peroxidase isozyme activities, and had 5-7 isozyme bands including their own key bands. Of the three variants, both G. elata f. elata and G. elata f. glauca had seven isozyme bands and G. elata f. viridis had five ones, i.e. G. elata f. elata and G. elata f. glauca had higher numbers of isozyme bands than G. elata f. viridis did. Of the isozyme bands in the three variants, five bands are the fundamental ones with their RF values standing at 0.06, 0.24,0.83,0.89,and 0.98, respectively. The similarity index between G. elata f. elata and G. elata f. glauca was 0.86, which indicated that the two variants had a closer genetic relationship; the index of G. elata f. glauca and G. elata f. viridis was 0.71, which indicted that the two variants had a distant genetic relationship.
【Fund】: 陕西省教育厅重点实验室重点科研项目资助(04JS32)
【CateGory Index】: S567.2
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