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《Acta Botanica Boreali-occidentalia Sinica》 2005-11
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Exploration of the Transformation Parameters of the Leaf Discs of Bergamot by Particle Bombardment in Virtue of Transient Expression of GUS Gene

ZHOU Chun-li~(1,3),GUO Wei-dong~(2*),WANG De-jie~1,YU Chu-lang~2,LU Mei~2,LI Yu-ping~1(1 College of Life Sciences,Jiangxi Normal University of Science and Technology,Nanchang,Jiangxi 330013,China;2 Academy of Life and Environment Science,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;3 Horticulture College,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China)  
Bergamot is a plant of high medicinal and ornamental values that belongs to Citrus medica L.var.sarcodactylis(Noot.) Swingle,but does not have many varieties.Thus it is necessary to conduct its genetic improvement and variety breeding.In order to apply particle bombardment to the genetic improvement and variety breeding of bergamot,this study,using GUS gene as the report gene,transformed GUS gene into the explants of Jinhua bergamot by particle bombardment;the transient expression of GUS gene in the leaf discs of bergamot were examined to analyze the effects of explant tenderness,gunshot,helium pressure,vacuum degree and bombardment frequency on the expression of GUS gene,and explore the optimal examination time.The results revealed that under the circumstances that the explants were young leaves,the gunshot was 6 cm,the helium pressure was 7.584×10~6 Pa and the vacuum degree was 88.046 kPa,the most active transient expression of GUS gene could be detected two days after particle bombarding.The bombarding frequency did not show significant influence on the transient expression of GUS gene.
【Fund】: 浙江省科技计划项目资助课题(2003C32043)
【CateGory Index】: S686;S567.19;
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