Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Botanica Boreali-occidentalia Sinica》 2005-11
Add to Favorite Get Latest Update

Exploration of the Transformation Parameters of the Leaf Discs of Bergamot by Particle Bombardment in Virtue of Transient Expression of GUS Gene

ZHOU Chun-li~(1,3),GUO Wei-dong~(2*),WANG De-jie~1,YU Chu-lang~2,LU Mei~2,LI Yu-ping~1(1 College of Life Sciences,Jiangxi Normal University of Science and Technology,Nanchang,Jiangxi 330013,China;2 Academy of Life and Environment Science,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;3 Horticulture College,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China)  
Bergamot is a plant of high medicinal and ornamental values that belongs to Citrus medica L.var.sarcodactylis(Noot.) Swingle,but does not have many varieties.Thus it is necessary to conduct its genetic improvement and variety breeding.In order to apply particle bombardment to the genetic improvement and variety breeding of bergamot,this study,using GUS gene as the report gene,transformed GUS gene into the explants of Jinhua bergamot by particle bombardment;the transient expression of GUS gene in the leaf discs of bergamot were examined to analyze the effects of explant tenderness,gunshot,helium pressure,vacuum degree and bombardment frequency on the expression of GUS gene,and explore the optimal examination time.The results revealed that under the circumstances that the explants were young leaves,the gunshot was 6 cm,the helium pressure was 7.584×10~6 Pa and the vacuum degree was 88.046 kPa,the most active transient expression of GUS gene could be detected two days after particle bombarding.The bombarding frequency did not show significant influence on the transient expression of GUS gene.
【Fund】: 浙江省科技计划项目资助课题(2003C32043)
【CateGory Index】: S686;S567.19;
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 ZHAO Wen-Ting1,2,WEI Jian-He2,LIU Xiao-Dong1,GAO Zhi-Hui2 1.College of Landscape Architecture,Northeast Forestry University,Harbin 150040;2.Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Malianwabei Road,Beijing 100193;China;Advance of the Main Methods and Applications of Plant Transient Expression System[J];Letters in Biotechnology;2013-02
2 LU Mei,YANG Jia′ni,ZHOU Duanxu,LI Youqiang (College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China);Biological characteristics of the pathogenic fungus causing anthracnose in fingered citron[J];Journal of Zhejiang Normal University(Natural Sciences);2011-03
3 Liu Haitao1,2,3,Zhang Chuanhong2,Ma Miao1,3,Zheng Yongqi2(1Life Science College of Shihezi University,Shihezi Xinjiang 832003;2Research Institute of Forestry,Chinese Academy of Forestry,Key Lab of Tree Breeding and Cultivation of State Forestry Administration,Beijing 100091;3Xinjiang BINGTUAN Key labotatory of Oasis Eco-agriculture,Shihezi Xinjiang 832003);Current Status and Research Progress in Genetically Modified Trees and Biosafety Management[J];Chinese Agricultural Science Bulletin;2009-05
【Citations】
Chinese Journal Full-text Database 7 Hits
1 ZHAO Yan 1~3 ,YU Yan Chun 1,3 ,QIAN Qian 3,① ,YAN Mei Xian 3,HUANG Da Nian 1,3,② (1 College of life science,Zhejiang University,Hangzhou 310029,China; 2 Biological engineering Department,Hangzhou University of Commerce,Hangzhou 310035,China; 3 Biotechnology Department,Key Laboratory for Rice Biology,Ministry of Agriculture,China National Rice Institute,Hangzhou 310006,China);Cotransformation of Rice by bar and cecropin B Gene Expression Cassettes Lacking Vector Backbone Sequences[J];Acta Genetica Sinica;2003-02
2 YUAN Ying,LIU De-pu,ZHENG Pei-he,et al.(Jilin Province Key Open Lab on Agrobiology,Gongzhuling136100,China);Factors Affecting the Biolistic Transformation of Commercial Corn Inbred Lines in Northeast China[J];Journal of Maize Sciences;2004-01
3 CHEN Bing-chu, ZHAO Bin, SHENTU Wen-yue ( 1. College of Chemistry and Life Sciences,Zhejiang Normal Universily , Jinhua Zhejiang 321004 ,China ;2. Zhejiang Senhe Seed Co. Ltd,Hangzhou Zhejiang 310020,China);Research on medium culture technology and some physiological index of Jinhua fingered citron[J];Journal of Zhejiang Normal University (Natural Sciences);2003-03
4 ZHENG Xiao-hua, WENG Xue-xiang(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China);Determination of volatile components in bergamot by gas chromatography-mass spectrometry with solid-phase microextraction[J];Journal of Zhejiang Normal University (Natural Sciences);2004-01
5 Zhu bing Huang Danian Yang Wei Xue Rui Xiao Han (China National Rice Research Institute, Hangzhou 310006) Tian Wenzhong Li Liangcai Dai Shunhong (Instituete of genetics, Academic Sinica);Prodution of Herbicide resistant Transgenic Rice Plants from Immature Embryos Using Biolistic Method[J];SCIENTIA AGRICUTURA SINICA;1996-06
6 Jin Xiaoing(Zheiang Normal University,Jinhua 321004).;The Exploitation and Utilization of Jindua Bergamot Resource[J];RESOURCE DEVELOPMENT & MARKET;1998-06
7 WU Shan 1, , LIANG Yue Rong 1,ZHU Yun Guo 2 ( 1Department of Tea Science, Zhejiang University, Hangzhou 310029; 2Scientz Institute of Instruments, Ningbo, Zhejiang 315010 );Application of Particle Bombardment in the Research of Plant Genetic Engineering[J];Plant Physiology Communications;2003-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Fang1,2, ZHANG Bin2, SHAN Lei2, BI Yu-ping2, XU Ze-yong3(1. School of Life Sciences, Shandong Normal University, Jinan 250014,China; 2. Plant Biology Technology Research Center, SAAS, Jinan 250100,China; 3. Oil Crops Research Institute ,CAAS,Wuhan 430062,China);An Improved Method for RNA Isolation from Peanut Seeds[J];Journal of Peanut Science;2002-04
2 LI Qingyun,ZHUANG Donghong,HU Zhong, HUANG Xiuli (Dept. of Biology, Shantou University, Shantou 515063, China);Extraction of DNA from Different Parts of Peanut[J];Journal of Peanut Science;2003-02
3 ZHOU Xiang-hui1,ZHUANG Dong-hong1,ZHENG Yi-xiong2,HOU Jian1,GUAN Xiu-qiong1(1. Department of Biology, Shantou University,Shantou 515063, China; 2. Zhongkai Agrotechnical College, Guangzhou 510225, China);Study on Shoot Induce of Different Cultivated Species Peanut(Arachis hypogaea L.)[J];Journal of Peanut Science;2003-S1
4 ZHAN Jie~1,YU Yong-chang~2,HE Long-fei~1,WU Rong-dong~1(1.Agri.College,Guangxi Uni.,Nanning 530005,China;2.Forestry Academy,Guangxi Uni.,Nanning 530005,China);An Optimum Method of Extracting DNA from Peanut Root Tips[J];Journal of Peanut Science;2006-03
5 YANG Yong-zhi,WANG Jian ZHANG Yan-ping ZHOU Yun SUN Hai-hong (The Biotechnology Institute of Qinghai Academy of Agriculture and Forestry,810016,Xining);View on Plant Exogenous Gene Transformation Technique[J];Science and Technology of Qinghai Agriculture and Forestry;2007-01
6 HUANG Chunqiong GUO Anping KONG Hua HE Lika (State Key Laboratory of Tropical Crops \ Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101);Application of Genetic Engineering in Breeding of Ornamental Plants[J];Chinese Journal of Tropical Agriculture;2006-02
7 HU Junyu1,2 WANG Jungang2 CAI Wenwei2 ZHANG Shuzhen2 LI Xiaojun1,2 (1 Agronomy College of Hainan University, Danzhou, Hainan 571737; 2 Institute of Tropical Bioscience and Biotechnology, CATAS/Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops, Haikou, Hainan 571101);Extraction Methods for Sugarcane Genomic RNA[J];Chinese Journal of Tropical Agriculture;2009-06
8 LI Jing1) YIN Junmei2) REN Yu2) YANG Guangsui2) (1 College of Agronomy, Hainan University, Danzhou, Hainan 571737; 2 Tropical Crops Genetic Resources Institute, CATAS/ Ministry of Agriculture Key Laboratory for Utilization of Tropical Crops Germplasm Resources, Danzhou, Hainan 571737);Extraction of Genomic DNA of Dendrobium with SDS[J];Chinese Journal of Tropical Agriculture;2009-12
9 CHEN Pinghua1) XU Liping1) WANG Hengbo1) CHEN Youqiang2) CHEN Rukai1) (1 Key Lab for Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002; 2 Fujian Normal University, Fuzhou, Fujian 350108);Genetic Improvement of Sugarcane and Roles of Legal Organizations for Detection of GMO[J];Chinese Journal of Tropical Agriculture;2009-12
10 YANG Jun1,2) REN Yu1,3) YANG Guangsui1,3) YIN Junmei1,3)(1 Tropical Crops Genetic Resources Institute,CATAS,Danzhou,Hainan 571737;2 College of Agronomy,Hainan University,Danzhou,Hainan 571737;3 Ministry of Agriculture Key Laboratory of Tropical Crop Germplasm Resources Utilization,Danzhou,Hainan 571737);Establishment and Optimization of Sequence-Related Amplified Polymorphism System for Phalaenopsis[J];Chinese Journal of Tropical Agriculture;2010-03
China Proceedings of conference Full-text Database 6 Hits
1 YIN Jun REN Jiang-ping LI Zhi-gang LI Lei ZHOU Su-mei WEI Li SONG Li (National Engineering Research For Wheat,Henan Aricultural University,Zhengzhou 450002,China);The study on transgenic wheat with the characteristic of pre-harvest sprouting resistance[A];[C];2007
2 WU Jie~1,ZHOU Yu~2,ZHANG Xue-mei~2,YAN Wen-zhao~1 (1.Institute of Biotechnology and Nuclear Technology,Sichuan Academy of Agriculture Sciences,Chengdu 610066,China;2.Chengdu Institute of biological products,Chengdu,610023);Transformation of heat shock protein gene(HspB-C)of helicobacter pylori into sweet potato varieties[A];[C];2009
3 Ma Yan-zhi~(1,2) Zhang Yu-xing~1 (1 College of Horticulture,Agricultural University of Hebei,Baoding Hebei 071001;2 Department of Biological Science, Tangshan Teacher's College,Tangshan Hebei 063000);Effect of Genome DNA Extracted from Different Tissues on RAPD in Pyrus L.[A];[C];2009
4 YAN Li-ping,XIA Yang,LIANG Hui-min,WANG Zhen-zhen,LI Shuang-yun, LIU Cui-lan,PANG Cai-hong (Shandong Provincial Academy of Forestry,Jinan 250014);Transconduct BADH Gene into Alfalfa and Salt Tolerance Analysis of Transgenic Generation[A];[C];2009
5 LI Qin TANG Fu Mei SHU chui (Yunnas Province Red River Tropical Agricultural Scienti Research Institute Zip Code 661,309);The Report on artichoketissue culture at the beginning of research[A];[C];2007
6 LIU Zefa~(1,2),TANG Yanying~1,LI Ling~1 SUN Xiaowu~1 (1.Landiscape and Horticultural Institute of Hu nan Agriculture University,Changsha Hunan 410128,China;3.Hunan XiangYan seed co.,LTD.,Changsha,Hunan,410000;2.Hunan University of Humanities,Science and Technology,Loudi,Hunan,417000,China);SRAP/RSAP Molecular Markers Study in Pumpkin(C.maxima L.) Varieties Hybrid Purity Detection[A];[C];2013
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhou Peng; Zhao Zhiying; Zheng Xueqin(National Key Biotechnology Lab for Trpica1 Crops,CATAS,Danzhou Hainan 571737);Reconstruction Method of Plant Expression Vector for Human α-IFN-2b Gene[J];CHINESE JOURNAL OF TROPICAL CROPS;1998-01
2 Zhao Zhiying; Zhou Peng; Zeng Xiansong; Zheng Xueqin (National Key Biotechnology Laboratory for Tropical Crops,CATAS, Danzhou, Hainan, 571737);Transformation of Ribozyme Gene into Papaya[J];CHINESE JOURNAL OF TROPICAL CROPS;1998-02
3 Yu Xianmei Liu Zhixin Zeng Huajin Peng Cunzhi ( National Key Laboratory for Tropical Crop Biotechnology, CATAS, Haikou 571101 Institute of Environment and Plant Protection, CATAS, Danzhou, Hainan 571737);Establishment of Transgenic and Regeneration System of Giant Granadilla (Passiflora quadrangularis L.)[J];Chinese Journal of Tropical Crops;2003-04
4 Lou Jingping Zhang Shuzhen Yang Benpeng (National Key Laboratory for Tropical Crop Biotechnology, CATAS, Haikou 571101);GFP Gene Transformation of Sugarcane Mediated by Agrobacterium tumefaciens[J];Chinese Journal of Tropical Crops;2003-04
5 He Yongrui Zhou Xiuping Peng Aihong Xu Zhongqiang Xu Lanzen Pen Zhuchun Cheng Shanchun(Citrus Research Institute, CAAS, Chongqing, 400712);Agrobacterium-Mediated Transformation of Citrus Embryoids and Mature Shoots[J];Chinese Journal of Tropical Crops;2004-01
6 He Chunping1,3,4,Wu Weihuai1,3,4,Yu Xianmei1,3,4,Zheng Xiaolan1,3,4,Li Rui1,3,4,Tu Sheng2 1 Environment and Plant Protection Institute,CATAS,Danzhou,Hainan 571737;2 College of Environment and Plant Protection,Hainan University,Danzhou,Hainan 571737;3 Ministry of Agriculture Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests,Danzhou,Hainan 571737;4 Hainan Key Laboratory for Detection and Control for Tropical Agricultural Pests,Danzhou,Hainan 571737,China.;Biological Characteristics of the Pathogenic Fungus Causing Anthracnose Infecting Rambutan[J];Chinese Journal of Tropical Crops;2010-02
7 Zeng Changying,Chen Xin,Lu Cheng,Wang Wenquan,Peng Ming Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou,Hainan 571101,China;Plant Stress Resistance-related miRNA and Its Application in Cassava[J];Chinese Journal of Tropical Crops;2010-02
8 ZhouPeng;ZhengXueqin(National Key Biotechnology Laboratory for Tropical Crops,CATAS,Danzhou,Hainan 57 1 737);EXPRESSION OF PRSV-CP GENE IN TRANSGENIC PAPAYA[J];CHINESE JOURNAL OF TROPICAL CROPS;1995-S1
9 ZHOU Chun-li1,3, GUO Wei-dong2*, LU Mei2, CHEN Jin2, LI Yu-ping1 ( 1. College of Life Sciences, Jiangxi Normal University of Science and Technology, Nanchang 330013, China; 2. Academy of Life and Environment Science, Zhejiang Normal University, Jinhua 321004, China; 3. College of Horticulture, Northwest Agricultural and Foresty University, Yangling 712100, China);Factors Effecting the Transformation of Citrus medica L. var. sarcodactlis Mediated by Agrobacterium[J];Journal of Tropical and Subtropical Botany;2006-05
10 PENG Ke,MEI Wen-li,WU Jiao,DAI Hao-fu(Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China);Flavones from the Stem of Aquilaria sinensis[J];Journal of Tropical and Subtropical Botany;2010-01
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 Cui Xudong;Zhang Bingyu;Ding Changjun;Su Xiaohua;State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry;;Plant Transgenic Technology and Its Application to Genetic Improvement of Forest Trees[J];World Forestry Research;2013-05
2 ZHAO Wen-Ting;GAO Zhi-Hui;MENG Dong;LIU Xiao-Dong;WEI Jian-He;Institute of Medicinal Plant Development,Chinese Academy of Medical Science & Peking Union Medical College;Northeast Forestry University;China Agricultural University;Hainan Branch,Institute of Medicinal Plant Development(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine),Chinese Academy of Medical Sciences & Peking Union Medical College;;Construction of the in vivo Validation Systems of Plant microRNA Functions[J];Letters in Biotechnology;2014-01
3 LU Mei,YE Mei-Juan,LING Dan-Yan,CHEN Wen-Rong(Coll.of Chem.and Life Sci.,Zhejiang Normal Uni.,Jinhua Zhejiang 321004);Factors Affecting Conidium Germination & Appressorium Formation of Camellia Anthracnose (Colletotrichum gloeosporioides)[J];Journal of Microbiology;2012-06
4 LIAO Fang-lei;CHEN Min-guan;SANG Dan;CHEN Wen-rong;GUO Wei-dong;College of Chemistry and Life Sciences in Zhejiang Normal University;;Genetic Diversity Analysis of Fingered Citron Germplasm by ISSR[J];Acta Horticulturae Sinica;2013-11
5 WU Li-fang;YANG Hai-yan;College of Biologic Resource and Environmental Science,Qujing Normal University;Chuxiong Normal University;Yunnan Province University Key Laboratory of Ethnobotany in Central Yunnan;;Study on Biological Characteristics of Colletotrichum Dracaenae-fragrantis sp. from Yunnan Province[J];Southwest China Journal of Agricultural Sciences;2014-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Shirun Zhang Juren Chen Huimin (Dept.of Biol.Shandong Univ.,Jinan);STUDY ON INDUCTION OF EMBRYOGENIC CALLUSE AND PLANTLET REGENERATION IN MAIZE[J];Journal of Shandong University;1990-01
2 Wang Gouying Zhang Hong Ding Qunxing Dai Jingrui Xie Youju (Beijing Agricultural University, Beijing 100094);Studies on Three Maize Gene Transfer Techniques[J];Chinese Journal of Biotechnology;1996-01
3 An Hanbing Zhu Zhen (Institute of Genetics,Academia Sinica,Beijing 100101);Application of Particle Bambardment in Plant Genetic Transformation[J];Progress in Biotechnology;1997-01
4 JIN Xiao _ ling, XU Li _ shan, ZHENG Xiao _ hua(Department of Biology, Zhejiang Normal University, Jinhua 321004, China);Chemical Compositions of Essential Oil from Bergamot[J];JOURNAL OF INSTRUMENTAL ANALYSIS;2000-04
5 Chen Lianghong Wang Xinwang Zhang Wenjun Zhang Xiaodong Hu Daofen Liu Guangtian(Dept. of Plant Genetics and Breeding, China Agricultural University, Beijing, 100094; Institute of Genetics, Chinese Academy of Sciences, Beijing, 100101; Beijing Academy of Agricultural Sciences, Beijing, 100081);Transformation of Genes Encoding High Molecular Weight Glutenin Subunit (HMW-GS)into Common Wheat (Triticum aestivum L. )[J];Acta Agronomica Sinica;1999-04
6 Xu Xinping Hu Ming Yi Lezhou Wei Jianwen Li Baojian(Biotechnology Research Center, Zhongshan University, Guangzhou 510275);Efficient Production of Fertile Transgenic Rice Following Particle Bombardment[J];Acta Agronomica Sinica;1999-06
7 DAI Shun-hong LI Liang-cai DING Yue-yun HE Si-jie CAO Shou-yun TIAN Wen-zhong (Institute of Genetics Chinese Academy of Sciences Beijing 100101);Multiple Gene Transformation of Rice Using the Biolistic Method[J];Acta Genetica Sinica;1998-04
8 LIANG Hui ZHAO Tie-han LI Liang-cai OUYANG Jun-wen TIAN Wen-zhong JIA Xu (Institute of Genetics Chinese Academy of Sciences Beijing 100101);Studies on Some Factors Affecting Transformation of Wheat Immature Embryos by Biolistic Bombardment[J];Acta Genetica Sinica;1998-05
9 HUA Zhi-Hua1, ZHU Xue-Feng1, LIN Hong-Sheng 1,2, GAO Zhen-Yu1, QIAN Qian1, YAN Mei-Xian1, HUANG Da-Nian1 (1. Biotechnology Department, China National Rice Research Inst itute and Key Laboratory for Rice Biology, Ministry of Agriculture, Hangzhou 3;Studies of the Integration and Expression of Exogenes in Transgenic Rice Obtained via Particle Bombardment Transformation[J];Acta Genetica Sinica;2001-11
10 FU Rongzhao CAO Guangcheng MA Jiansheng LI Wenbin SUN Yongru (Institute of Genetics ,Chinese Academy of Sciences Beijing 100101) CHEN Zhankuan YI Minglin ZHI Yubao LIN Zuoji (Henan Agricultural Academy Zhengzhou 450002);Preliminary Study of Transferring Engineered Male Sterile Gene intoWheat via Microprojectile Bombardment[J];ACTA GENETICA SINICA;1997-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved