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《Acta Botanica Boreali-Occidentalia Sinica》 2006-01
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Preliminary Study of Action Mechanism of Tomatine Toxicity to Helicoverpa armigera

DUAN Jiang-yan~1,DU Li-ming~2,WU Hao~2,YUAN Li-huan~1,GAO Gang~1(1 College of Life Science,Shanxi Teachers University,Linfen,Shanxi 041004,China;2 Analytical and Testing Center,Shanxi Teachers University,Linfen,Shanxi 041004,China)  
The spectra of tomatine and calmodulin(CaM) were investigated by fluorescent spectrometry and the action mechanism of tomatine toxicity were analyzed from the angle of calmodulin.The results showed that(1) tomatine was toxic to Helicoverpa armigera and its lethality to the insect increased as its concentration increased and its feeding time prolonged;(2) Tomatine exerted great influence on endosomatic calmodulin of the insect,increased tomatine concentration resulting in gradually declined endosomatic calmodulin content;(3) Calcium ion addition increased calmodulin rigidity so that red shift occurred in the fluorescent spectra (from 35 nm to 416.58 nm);(4) After its addition,tomatine damaged CaM-Ca~(2+) rigidity so that blue shift occurred in the fluorescent spectra(from 416.58 nm to 377.65 nm).Those above tell that tomatine is probably used as an antagon to calmodulin and the Ca~(2+)-activitated site of calmodulin take effect by affecting the combination of calmodulin with target enzymes.This study provided scientific basis to studying the insect-disinfecting mechanism of tomatine.
【Fund】: 山西省青年科技基金项目(20021044)
【CateGory Index】: S433
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