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《Acta Botanica Boreali-Occidentalia Sinica》 2006-02
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Tissue Culture and High-efficiency Plant Regeneration of Arctium lappa

HE Wen-ting~1,HOU Sui-wen~(1,2*),WANG Chong-ying~1(1 School of Life Science,Lanzhou University,Lanzhou 730000,China;2 Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China)  
Different Arctium lappa L.explants and the compounding proportions of different hormones were comparatively studied in tissue culture and thereafter a high-efficiency plant-regeneration system by in vitro A.lappa culture.On MS mediums containing 2.0 mg/L 2,4-D and 0.5~2.0 mg/L BA,cotyledon and hypocotyl pieces could be induced to produce callus at 87%~100%;On MS medium containing 1.0~3.0 mg/L NAA and 0.5~2.0 mg/L BA,the callues could indirectly differentiate or the explants could directly differentiate to form adventitious buds and the differentiation rate of the calli could reached as high as 100%;The hypocotyls differentiated at a higher rate than the cotyledons and their direct differentiation rate amounted to 77.3% on MS medium containing 1.0 mg/L NAA and 1.0 mg/L BA.A.lappa was histochemically found capable of regenerating by two approaches,organogenesis and embryogenesis.Well-growing adventitious buds were inoculated on 1/2 MS medium containing 1.0 mg/L NAA and 1.0 mg/L BA for their rooting and thereafter transplanted,and their survival rate reached 93.3%.It approximately took 13 weeks to complete the transition from callus induction to in vitro plantlet transplantation to perlite matrix.The in vitro plantlets flowered in the following year and presented a normal morphology in their growth.
【Fund】: 兰州大学交叉学科青年创新研究基金项目(Lzu-200312);; 甘肃省自然科学基金项目(ZS011-A-25-009-Z);; 中国科学院近代物理研究所所长基金项目(ZY-050605)
【CateGory Index】: S631.9
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