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《Acta Botanica Boreali-Occidentalia Sinica》 2006-04
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RAPD Analysis of the Crossing Rate of the Spring Chinese Cabbage Variety Guanchun (Brassica campestris)

WANG Qi,ZHANG Lu-gang,ZHANG Zhan-feng,HUI Mai-xia,ZHANG Ming-ke (College of Horticulture ,Northwest Sci-Tech University of Agricuture and Forestry, Yangling,Shaanxi 712100,China)  
Genomic DNA was extracted from 'Guanchun' ,a spring Chinese cabbage variety,and its parents and then RAPD amplified with 320 random primers ;Five primers ,S4 ,S47 ,S73 ,S134 and S494,which were capable of telling between the parents and their progenies, were screened out of the primers. S4 could produce S4-370, the characteristic band of the male parent ;S47 and S134 could produce S47-700 and S134-1200,the characteristic bands of the female parent;and S73 and S494 could produce S73-660 ,S73-730 ,S494-400 and S494-1770, the complementary characteristic bands of the parents,all of which appeared in the progenies. The characteristic bands produced by the five primers were used to construct RAPD fingerprints specific to 'Guanchun' and its parents. 134 seeds of 'Guanchun' were identified in their purities to come from two male parental plants and four female parental plants,which was consistent with what was identified in the field. This further verified the feasibility of four methods to identify hybrid seeds of Chinese cabbage in purity.
【Fund】: 国家863项目(2003AA207120)
【CateGory Index】: S634.1
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