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《Acta Botanica Boreali-Occidentalia Sinica》 2006-05
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Active Anti-microbial Ingredients in Mikania micrantha Stems and Leaves

YU Xin-ping~1,FENG Jun-tao~(1,2),LIU Xiao-ming~1,ZHUANG Shi-hong~(1,2*),ZHANG Xing~(1,2)(1 Research and Development Center of Biorational Pesticide,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Shaanxi Research Center of Biopesticide Technology and Engineering,Yangling,Shaanxi 712100,China)  
A substance,which was capable of well inhibiting the germination of four pathogens,Pseudoperonispora cubensis,Colletotrichum lagenarium,Fusarium graminearum and Exserohilum turcium was isolated by bioactivity-guided isolation with Rhizoctonia cerealis as the indicator.Where its concentration was set at 0.50 mg·mL~(-1),its inhibitive rates to P.cubensis,C.lagenarium,F.graminearum and E.turcium reached above 70%.It EC_(50) to Botrytis cirerea and where its concentration was set at 2 000 mg·kg~(-1),its therapeutic and protective efficacies separately reached 68.6% and 48.7%,higher than those of the control procymidon.The substance was identified by melting point test,IR,MS,~1HNMR and ~(13)CNMR to be stigmasterol,which was isolated from Mikania micrantha stems and leaves for the first time.
【Key Words】： Mikania micrantha anti-microbial activity stigmasterol
【Fund】： 国家“十五”攻关项目(2002BA516A04);; 陕西省农业科技攻关项目(2004K01-G7-02);; 西北农林科技大学青年科研专项项目
【CateGory Index】： S482.29
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