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《Acta Botanica Boreali-Occidentalia Sinica》 2006-09
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In Vitro Culture and Regeneration System of Pepper(Capsicum annuum)

ZHANG Jin-wen~1,FAN Xing-zhong~2,WANG Ying~2,WANG Han-ning~(1*)MENG Ya-xiong~1,LONG Feng~3(1 College of Agronomy,Gansu Key Laboratory of Crop Genetic Improvement Sci-Tech Germplasm Enhancement,Gansu Agricultural University,Lanzhou 730070,China;2 Jiuquan Agricultural Research Institute,Jiuquan,Gansu 735000,China;3 College of Life Science,Lanzhou University,Lanzhou 730000,China)  
Nine pepper varieties(Capsicum annuum) were used to investigate the effects of such factors as different hormone combinations,genotypes,explant types and seedling ages and AgNO_3 on adventitious bud differentiation and elongation of pepper explants.It was shown that the 6-BA/IAA ratio was conducive to the differentiation and regeneration of pepper explants at 3∶1 and unsuitable for the elongation of the regenerative buds;different pepper varieties differed greatly in their regeneration capacities which ranged within 13.3%~90.0%.In pepper,the cotyledons had stronger regenerative capacity than the hypocotyls and thus were good explants;the explants from 12~16 day old seedlings had a high differentiation rate;the addition of AgNO_3 at 4 mg·L~(-1) could raise the average differentiation rate by 16.9%.Comparatively speaking,the optimal medium for the differentiation of pepper buds was MB_5+5 mg·L~(-1) 6-BA+0.5 mg·L~(-1) IAA+4 mg·L~(-1)AgNO_3,the medium for the bud elongation was MB_5+3 mg·L~(-1) 6-BA+1.0 mg·L~(-1) IAA+2 mg·L~(-1) GA_3+4 mg·L~(-1) AgNO_3 and the medium for rooting culture was 1/2 MS+0.2 mg·L~(-1) IAA+0.1 mg·L~(-1)NAA.
【Fund】: 甘肃省自然科学基金项目(ZS991-A21-029-N)
【CateGory Index】: S641.3;Q943.1
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