Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Botanica Boreali-Occidentalia Sinica》 2006-11
Add to Favorite Get Latest Update

SSH Specific Expression of the Internodes Beneath the Spikes of Thermo-Sensitive Genic Male Sterility Rice

XIAO Hui-hai,HE An-na,LIU Jun-fei(Department of Life Science,Hunan University of Arts and Science,Changde,Hunan 415000,China)  
'Changxuan 3S' is a long-spike neck and thermo-sensitive genic male sterility rice variety bred with the rice variety 'Peiai 64S' by 350 GY60 γ-ray radiation mutagenesis.A suppression subtractive hybridization between the internodes of 'Changxuan 3S' at the biuncleate pollen stage as the testers populations and the internodes of 'Peiai 64S' at the binucleate pollen stage as the drivers was conducted and then a subtracted cDNA library composed of 130 independent clones was constructed with cDNA of 'Changxuan 3S' modified with cDNA of 'Peiai 64S';randomly-chosen 96 recombinant plasmids were screened with cDNA of 'Peiai 64S' and cDNAs of 'Changxuan 3S' before suppression subtraction hybridization and their cDNAs after forward and backward-direction suppression subtractive hybridizations as the template-marking probes and twenty positive candidate clones were chosen;eight candidate clones taken from these positive candidate clones were analyzed by Northern blotting and one of them were proved to represent the specific expression gene of the internodes beneath the spike of 'Changxuan 3S';the sequencing and homology comparison of the gene indicated that it was related to signal transmission.This specifically expressed cDNA fragment is helpful to further reveal the elongation molecular mechanism of the internodes beneath the spikes and has laid down a foundation for improving sterility rice lines.
【Fund】: 湖南省教育厅资助项目(04C417)
【CateGory Index】: S511.21
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 9 Hits
1 DONG Yun zhou 1,DUAN Sheng jun 2\j(1.Biotechnology Research Center,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.Institute of Millet Crops,Hebei Academy of Agricultural Sciences,Shijiazhuang 050031,China);Production of transgenic millet plants via particle bombardment[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;2000-02
2 YAO Fang yin 1,LI Guang xian 1,YANG Lei 1, LI Zhong hua 2,ZHAI Hong mei 2,YANG Hai xu 2 (1 Shandong Rice Research Institute,Jining,Shandong 272017,China;2 Jining Seed Corporation,Jining,Shandong 272037,China);Regeneration of transgenic rice plants after trans-formation of a bacterial blight resistance gene Xa21 by using Agrobacterium-mediated system[J];Acta Botanica Boreali-occidentalia Sinica;2002-05
3 LIANG Man-Zhong,ZHONG Wei-Hua,XU Meng-Liang,CHEN Liang-Bi (Life Science College,Hunan Normal University,Changsha 410081,Hunan,China);Genetic Analysis of TGMS Rice Shuangdi Pei eS with eui Gene[J];Acta Agronomica Sinica;2004-08
4 ZHONG Wei-Hua,XIAO Hui-Hai,LIANG Man-Zhong,CHEN Liang-Bi (College of Life Science,Hunan Normal University,Changsha 41 0081,Hunan,China);Cytological Studies on Uppermost Internode of TGMS Rice with eui Gene[J];Acta Agronomica Sinica;2005-05
5 Liao Changli Ni Keyu Liu Yuankun Huang Zonghong (Guizhou Academy of Agricultural Sciences, Guiyang);STUDIES ON HEREDITY AND UTILIZATON OF GRLC RECESSIVE TALL TYPE IN RICE——Ⅰ. Characters of plant and culm type of Grlc and its testcross F_1[J];Southwest China Journal of Agricultural Sciences;1988-03
6 Sun Lihua;Wang Yuefang;Jiang Ning(Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences Nanjing 210014)Li Hebiao(Institute of Food Crops JAAS Nanjing);A Recessive Tall Culm Somatic Mutant with Wide Compatibility in Rice (Oryza sativa L.)[J];ACTA GENETICA SINICA;1994-01
7 HE Zuhua SHEN Zongtan (Zhejiang Agricultural University,Hangzhou 310029);Inheritance of Panicle Exsertion and Improvment of Male Sterile Line in Rice[J];Chinese Journal of Rice Science;1991-01
8 XIAO Hui-hai1,2, ZHONG Wei-hua1, LIANG Man-zhong1, XU Meng-liang1, CHEN Liang-bi1 (1College of Life Science, Hunan Normal University, Changsha 410081; 2College of Sciences, Hunan Agricultural University, Changsha 410182 );Effect of Temperature on the eui Gene Expression of TGMS Rice[J];Scientia Agricultura Sinica;2005-02
9 GUO Xin Hong 1 JIANG Xiao Cheng 1 PAN Xiao Ling 2 DAI Yu Chi 1 JIANG Wei Ming 1 CHEN Liang Bi 1 ( 1 College of Life Science of Hunan Normal University , Changsha 410081; 2 Reasearch Institute of Arid Ecological Environment of Xinji;Isolation and Cloning of cDNA Related with the Genes of Halonxylon ammodendron (Mey.) Bge. Seedlings Induced by Osmotic Stress by Suppression Subtractive Hybridization[J];Acta Photophysiologica Sinica;2001-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Lan-zhen et al(Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 400712);Construction of cDNA Library and its Application in the Study on Plant Resistance[J];Journal of Anhui Agricultural Sciences;2007-03
2 XIAO Hui-hai (Department of Biology,Hunan University of Arts and Science,Changde,Hunan 415000);Effects of Rice eui Gene on Several Agronomic Traits of Rice Male Sterile Line Changxuan 3S[J];Journal of Anhui Agricultural Sciences;2008-15
3 LIAO Zi-rong et al(College of Agriculture,Hainan Univesity,Danzhou,Hainan 571737);Study on the Genetic Characteristics of a Biomass Mutant in Oryza sativa[J];Journal of Anhui Agricultural Sciences;2008-22
4 CHEN Zhuo et al(Department of Biology Engineering and Technology,Qingdao Science and Technology University,Qingdao,Shandong 266042);Analysis on Expressed Sequence Tags(EST) and Research Progress of Algae EST Library[J];Journal of Anhui Agricultural Sciences;2009-05
5 CHEN Jiang-dao et al(Fengtai County Rice Stock Seed Farm in Huainan City,Anhui Province,Huainan,Anhui 232100);Effects of Sowing Time and Transplanting Density on the Growth Period,Yield and Component Factors of Wandao 68[J];Journal of Anhui Agricultural Sciences;2009-19
6 Huang Zhongging (Anhui Agricultural College);THE EFFECTS OF THICKNESS ON QUALITY OF RICE SEEDLINGS[J];Journal of Anhui Agricultural University;1988-01
7 WANG Jing-tao1,WEI Chao-ling2(1.School of Resources and Environment,Anhui Agricultural University,Hefei 230036; 2.School of Tea & Food Science and Technology,Anhui Agricultural University,Hefei 230036);Effects of meteorological factors on yield and its components of middle indica hybrid rice in main production area of Anhui Province[J];Journal of Anhui Agricultural University;2009-03
8 Cai Yingfan; Mo Jianchuan; Zeng Yu; Ren Weiwei; Miao Chen; Xu Ying;Wang Shenghua; Chen Fang.Life College of Sichuan University, Chengdu,610064,P.R.China.;Cloning of cDNAs associated with the development of pigment gland of Gossypium by suppression subtractive hybridization.[J];Journal of Beijing Forestry University;2003-03
9 LIU Zhi-xiong(College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025,China;College of Life Science,Central China Normal University,Wuhan,Hubei 430079,China)LI Xing-run(College of Life Science,Central China Normal University,Wuhan,Hubei 430079,China);Characterization of Fertility Alteration in Four Environment-sensitive Genic Male-sterile Rices under Artificial Temperature Change[J];Journal of Yangtze University(Natural Science Edition)Agricultural Science Volume;2008-02
10 Xu Haiguang;Zhou Houjun (School of Agronomy,Hebei Agric Univ, Baoding 071001);The Effect 0f Using Nitrogen Fertilizer on the Growth and Development of Rice after Wheat in Different Stages[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-02
China Proceedings of conference Full-text Database 5 Hits
1 ZHANG Shu-biao YANG Ren-cui HUANG Rong-hua ZHANG Qing-qi (Institute of Genetics & Crop Breeding,Fujian Agriculture and Forestry University,Fujian Fuzhou 350002,China);Achievement in rice eui gene and eui hybrids Studies[A];[C];2005
2 YANG Zhong-yi, CAO Yong-sheng, SU Yan, LIU Xiao-li, LI Hua-hui, LU Yi-xuan (Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650231,China;Institute of Crop Sciences, China Academy of Agricultural Sciences, Beijing 100081,China);Classifcation and method of Indica and Japonica of China cultivated rice (review)[A];[C];2006
3 Yang rencui (Fujian Agriculture and Forestry University, Fuzhou ,Fujian 350002);Genetic Evaluation and Breeding Utilization of Rice eui-Germplasm[A];[C];2004
4 Fan Shuguo, Zhang Zaijun, Liu Lin, Liu Hongxian, Liang Chengye (South China Institute of Botany, the Chinese Academy of Sciences, Guangzhou 510650);THE SPECIES,GEOGRAPHICAL DISTRIBUTION OF WILD RICE AND THEIR BIOLOGICAL CHARACTERISTICS IN CHINA[A];[C];1998
5 Fan Shuguo, Zhang Zaijun, Liu Lin, Liu Hongxian, Liang Chengye (South China Institute of Botany, the Chinese Academy of Sciences, Guangzhou 510650);CONSERVATION OF WILD RICE GENETIC RESOURCES IN CHINA AND THEIR UTILIZATION IN BREEDING[A];[C];1998
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HUANG Rong hua, ZHANG Qing qi, ZHANG Shu biao, YANG Su lan,YANG Ren cui (Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);A preliminary study on the development of eui-CMS lines in rice by radiation induced mutation[J];Journal of Fujian Agricultural University(Natural Science);2001-02
2 Wu Shibi, Zhang Qihua ( Quanzhou Institute of Agricultural Sciences );STUDY ON INDUCED MUTATION LEADING TO OBTAINING A RECESSIVE TALL-CULM GENE IN RICE[J];;1988-01
3 CHEN Liang-bi1,ZHOU Guang-qia1,LI Yang-sheng2 ,TAN Zhou-ci1, XU Meng-liang1,JIANG Xiao-cheng1,LIANG Man-zhong1,HUANG Liang1 ( 1 .College of L ife Sciences,Hunan Normal University,Changsha41 0 0 81 ,Hunan,China;2 .Changsha Institute of Agricultural Mode;Progress in Applied Techniques of Resisting and Reducing Disaster on Rice[J];LIFE SCIENCE RESEARCH;2000-01
4 ZHOU Guang qia, CHEN Liang bi, LIANG Man zhong, XU Meng liang (College of Life Sciences, Hunan Normal University, Changsha 410081,Hunan,China);Development of TGMS Rice with eui-Gene Coincided with Double Low Critical Temperature[J];Life Science Research;2000-04
5 XU Meng liang,CHEN Liang bi,ZHOU Guang qia (Life Science College of Hunan Normal University,Hunan Institute of Biology Research,Changsha 410081, Hunan,China);Selection of a New Sterile Material Whose Critical Temperature Inducing Sterility is Low from Rice T(P)GMS Peiai 64S Population[J];LIFE SCIENCE RESEARCH;1999-02
6 XU Meng-Liang, CHEN Liang-Bi, ZHOU Guang-Qia, LIANG Man-Zhong (College of Life Science, Hu'nan Normal University, Changsha 410081,China).;Temperature Effects on Fertility of Rice TGMS Line Between 96-5-2S with Double Low Critical Temperature Values and Peiai 64S[J];Acta Ecologica Sinica;2002-04
7 Gu Keyu Zhai Huqu Department of Agronomy,Nanjing Agricultural University, Nanjing 210095;Advances in the Study on the Suppression Subtractive Hybridization[J];BIOTECHNOLOGY INFORMATION;1999-02
8 Chen Liang-bi;Li Xun-zheng;Gaung-qia Department of Biology, Hunan Normal University, Changsha 410006;Effects of Temperature on the Sterility of Rice in Photo-sensitive and Thermo-sensitive Male Sterile Lines[J];Acta Agronomica Sinica;1993-01
9 LIANG Man-Zhong,ZHONG Wei-Hua,XU Meng-Liang,CHEN Liang-Bi (Life Science College,Hunan Normal University,Changsha 410081,Hunan,China);Genetic Analysis of TGMS Rice Shuangdi Pei eS with eui Gene[J];Acta Agronomica Sinica;2004-08
10 Liao Changli Ni Keyu Liu Yuankun Huang Zonghong (Guizhou Academy of Agricultural Sciences, Guiyang);STUDIES ON HEREDITY AND UTILIZATON OF GRLC RECESSIVE TALL TYPE IN RICE——Ⅰ. Characters of plant and culm type of Grlc and its testcross F_1[J];Southwest China Journal of Agricultural Sciences;1988-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved