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《Acta Botanica Boreali-Occidentalia Sinica》 2007-02
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CBF1 Gene Transgenic Strawberry and Increase Freezing Tolerance

JIN Wan-mei,DONG Jing,YIN Shu-ping,YAN Ai-ling,CHEN Mei-xiang(Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China)  
To improve strawberry(Fragaria ananassa Duch.) freezing tolerance,cultivars "Honeoye" was studied by genetic transformation.An efficient method of genetic transformation of strawberry was leaf disc by Agrobacterium-mediated method.Transformants were obtained using a binary vector plasmid pBPCBF1 which contains a cauliflower mosaic virus 35S(CaMV35S) promoter driven CBF1(C-repeat binding factor) gene.Adventitious shoots were induced on MS+3.0 mg/L 6-BA+0.1 mg/L 2,4-D medium supplemented with 25 mg/L kanamycin.Results of molecular analysis by PCR and Southern blots confirmed the integration of CBF1 gene into the genome of the strawberry.Electrolyte leakage tests showed that transgenic plants of electrolyte leakage ratio were lower than control's.Plant survival tests demonstrated that 65% of transgenic plants were wilting and 91% of control plants were wilting under-2~0℃ for 7 days,74% of transgenic plants were survival and 18% of control plants were survival under 22~25℃.The results showed that expression of CBF1 gene increased the freezing tolerance of transgenic strawberry.
【Fund】: 北京市自然科学基金项目(5052010)
【CateGory Index】: S668.4
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【References】
Chinese Journal Full-text Database 10 Hits
1 XU Chun-bo et al(Grassland Research Institute of Chinese Academy of Agricultural Sciences,Hohhot,Inner Mongolia 010010);Research Progress on CBF Transcription Factor Applied to Genetic Engineering in China[J];Journal of Anhui Agricultural Sciences;2010-04
2 WANG Lian-rong et al (Horticulture College,Hebei North University,Zhangjiakou,Heibei 075131);Research Progress on Frost Resistance and Frost-resistant Measures of Fruit Tree[J];Journal of Anhui Agricultural Sciences;2010-18
3 WANG Min-hui et al(Key Laboratory of Crop Germplasm & Biotechnology,Ministry of Agriculture,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081);Researching Progress of Vernalization-related Genes in Triticum aestivum L.[J];Journal of Anhui Agricultural Sciences;2011-25
4 LI Zhi-liang1,2,3,WU Zhong-yi2,YE Jia1,YANG Qing3,ZHANG Xiu-hai2,HUANG Cong-lin2(1.Department of Biology Science,Handan College,Handan,Hebei 056005;2.Beijing Research Center of Agro-Biotechnology,Beijing 100097;3.College of Life Science,Nanjing Agricultural University,Nanjing,Jiangsu 210095);Study Advancements of DREB Transcription Factors and its Application in Plant Stress Tolerance Genetic Engineering[J];Northern Horticulture;2010-20
5 Sha Lina1,Guo Zhaokui1,2,Wan Xiuqing2,Cheng Hongmei3(1.Heilongjiang August First Land reclamation university,Daqing 163319;2.Heilongjiang Tobacco Research Institute,Mudanjiang 157011;3.Chinese Academy of Agricultural Sciences Biotechnology Research Institute);Test of the Cold-resistance Indicators of the CBF Genetically Modified Tobacco[J];Forest By-Product and Speciality in China;2009-01
6 WANG Duojia1,CANG Jing1,MU Yongchao1,ZENG Yan1,XU Ping2,YU Jing1(1.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China;2.Bureau of Forestry in Zoucheng,Zoucheng Shandong 273500,China);Research advance on cold-resistant gene in plants[J];Journal of Northeast Agricultural University;2009-10
7 CHEN Ru-gang,GONG Zhen-hui,LU Ming-hui,LI Da-wei,HUANG Wei(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China);Research Advance in the Cold-resistance Genetic Engineering in Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2008-06
8 SUN Zhong-lin,GU Di-zhou,ZHU Jun-yi,JIANG Yun-tian,FENG Ying,YUAN Yuan(Department of Biology,Tonghua Normal University,Tonghua 134002,China);Study on the growth trait of Fragaria orientalis var. concolor by petal induction[J];Guangdong Agricultural Sciences;2010-11
9 FU Shao-ping1,LI Rui-mei1,JI Yi-meng1,2,HUI Du-juan1,2,GUO Jian-chun1(1.Institute of Tropical Biosciences and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China; 2.College of Agriculture,Hainan University,Haikou 570228,China);Summary of researches on plant molecular breeding for cold resistance[J];Guangdong Agricultural Sciences;2011-07
10 Zhu Haisheng 1,2 Li Yongping 1,2 Wen Qingfang 1,2 Pan Dongming 3 Lin Yizhang 3 1 Vegetable Research Center,Fujian Academy of Agricultural Sciences,Fuzhou,350013; 2 Crops Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,350013; 3 College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,350002;Advances on Transformation of Strawberry[J];Genomics and Applied Biology;2009-02
China Proceedings of conference Full-text Database 2 Hits
1 GUO Bao-sheng , ZHAO Cun-peng , WANG Kai-hui , LIU Su-en , GENG Jun-yi;Selection of Transgenic Cotton Carrying CaM Gene[A];[C];2013
2 JIANG An~1 ZHANG Jian FAN Yan CHEN Jing HE Wei XIANG Bai-ju~* (Chong Qing Academy of Animal Husbandry Sciences,RongChang,ChongQing 400015);Cloning of Arabidopsis CBFl Gene and Construction of Plant Expression Vector[A];[C];2011
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Yin Shuping1 Jin Wanmei2 Wang Ping2 Meng Fanhong2 Han Zhenhai1(1.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094,China; 2. Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Science, Beijing 100094,China);Effects of Thidiazuron (TDZ) on Inducing Adventitious Shoot of Strawberry In Vitro[J];Journal of Agricultural Biotechnology;2003-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FAN Jing1,2,LIU Qing-zhong2,ZHANG Jun-lin2,3,WANG Jin1(1.Faculty of Landscape Architecture,Southwest Forestry College,Kunming 650224,China;2.Shandong Institute of Pomology,Taian 271000,China;3.Department of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China);A Modified Method for Genomic DNA Extraction from Juglans regia[J];Shandong Agricultural Sciences;2008-06
2 Huang Yan1 Guo Qingshui1 Xu Lixin1* Chen Shoucai2(1 College of Life science and Agriculture of Hainan University,Haikou 570228;2 Chinese Academy of Tropical Agriculture Science,Danzhou 571101);Comparision of three methods of Genomic DNA Extraction from rubber tree[J];Anhui Agricultural Science Bulletin;2008-07
3 YI Qing-ping et al (Department of Bio-engineering,Jingchu College of Technology,Jingmen,Hubei 448200);Advances of Genome DNA Extraction and Purification in Plants[J];Journal of Anhui Agricultural Sciences;2007-25
4 ZHOU Jing et al (Department of Immunology, Basic Medical College of Jilin University, Changchun, Jilin 130021);Study on the Transformation of Anthurium andreanum by Agribacterium tumefacien -mediated CBF1 Gene[J];Journal of Anhui Agricultural Sciences;2008-08
5 LIU Xiao-dan et al (College of Bio-engineering,University of Agriculture S&T of Jilin,Jilin,Jilin 132101);Roles of CBF Transcription Factor in Enhancing Stress Resistance of Plant[J];Journal of Anhui Agricultural Sciences;2009-32
6 XU Chun-bo et al(Grassland Research Institute of Chinese Academy of Agricultural Sciences,Hohhot,Inner Mongolia 010010);Research Progress on CBF Transcription Factor Applied to Genetic Engineering in China[J];Journal of Anhui Agricultural Sciences;2010-04
7 YING Yi-yi et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101);Comparison on Several Extraction Methods of Genomic DNA from Hevea brasiliensis[J];Journal of Anhui Agricultural Sciences;2010-17
8 JIA Ai-min et al(Agricultural College,Sichuan Agricultural University,Chengdu,Sichuan 611130);Research Progress of Transgenic Work in Tobaccos[J];Journal of Anhui Agricultural Sciences;2011-11
9 WANG Min-hui et al(Key Laboratory of Crop Germplasm & Biotechnology,Ministry of Agriculture,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081);Researching Progress of Vernalization-related Genes in Triticum aestivum L.[J];Journal of Anhui Agricultural Sciences;2011-25
10 BEI Zhan-lin,REN Xian,LEI Qian,ZHAO Hui-wei,FU Pei-yun(Department of Biological Science and Engineering,North Nationality University,Yinchuan,Ningxia 750021,China);Method Suitable for the RAPD Analysis of Ningxia Lycium bararum with DNA Highly Effective Extraction[J];Northern Horticulture;2009-09
China Proceedings of conference Full-text Database 6 Hits
1 Zhang Huping~1 Niu Jianxin~1 Wang Guo'an~2 Hu Haifang~2 Ma Binggang~1 Zhu Jun~1 (~1Department of Horticulture.College of Agriculture.Shihezi University,Shihezi 832003,China;~2 Xinjiang Forestry Academy.Urumqi 830000,China);Establishment of Optimal Reaction System of RAPD in Walnut[A];[C];2003
2 ;山东板栗遗传多样性SSR分析[A];[C];2006
3 By X. P. WEN, X. M. PANG and X. X. DENG National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China Bioengineering Center, Guizhou University, Guiyang 550025, China;Characterization of genetic relationships of Rosa roxburghii Tratt and its relatives using morphological traits,RAPD and AFLP markers[A];[C];2005
4 Xiao-Peng Wen~a,Xiao-Ming Pang~(be),Narumi Matsuda~c,Masayuki Kita~b, Hiromichi lnoue~b,Yu-Jin Hao~d,Chikako Honda~b,Takaya Moriguchi~b a Guizhou University,Guiyang,Guizhou 550025,China b National Institute of Fruit Tree Science,Tsukuba,Ibaraki 305-8605,Japan c Department of Agro-Production Science,Yamagata,General Agricultural Research Center,Sagae,Yamagata,991-0043,Japan d Shandong Agricultural University,Taian,Shandong 271018,China e Key laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083,China;Over-expression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers[A];[C];2010
5 XIANG Bai-ju~1,JUN Li-cheng,ZHANG Jian,FAN Yan,LUO Yi,JIANG An~* (Chong Qing Academy of Animal Husbandry Sciences,RongChang,ChongQing,400015);Study on the Transformation of maize SAUMZ1 by Agribacterium tumefacien-mediated CBF1 Gene[A];[C];2011
6 JIANG An~1 ZHANG Jian FAN Yan CHEN Jing HE Wei XIANG Bai-ju~* (Chong Qing Academy of Animal Husbandry Sciences,RongChang,ChongQing 400015);Cloning of Arabidopsis CBFl Gene and Construction of Plant Expression Vector[A];[C];2011
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Xiao-ju1,HONG Hai-bo2,LI Min1,LIU Yuan1,YANG Ke-qiang 1(1.College of Forestry,Shandong Agricultural University,Taian 271018,China;2.Haidong Agricultural Extension Center in Qinghai,Haidong 810600,China);An Improved CTAB Method for Extracting Total RNA from Walnut[J];Shandong Agricultural Sciences;2008-01
2 LI Li-bin1,LIU Li-feng1,WANG Dian-feng2,LIU Guo-ming3,GAO Jian-wei1(1.Institute of Vegetables,Shandong Academy of Agricultural Sciences,Jinan 250100,China; 2.Jinan Agricultural Bureau,Jinan 250021,China;3.Taitou Town Comprehensive Agricultural Service Station of Shouguang City,Shouguang 262735,China);Cloning and Phylogeny Analysis of Chinese Cabbage Gene CBF4[J];Shandong Agricultural Sciences;2009-04
3 LIU Jing1,ZHANG Yuan-hu1,LIU Qing-zhong2,GAO Yu-jiao1(1.College of Life Science,Shandong Agricultural University/State Key Laboratory of Crop Biology,Taian 271018,China; 2.Shandong Institute of Pomology,Taian 271000,China);Effects of Antibiotics on Leaf Regeneration of Apple Cultivar White Winter Pearmain[J];Shandong Agricultural Sciences;2009-07
4 QIU Qian-dong1,Lǚ Xiao-zhen2,ZANG De-kui1*,ZHANG Lei1,ZHANG Li-cai1,DU Shu-hui1(1.Forestry College,Shandong Agricultural University,Taian 271018,China;2.Hangzhou Landscape Engineering Co.Ltd.,Hangzhou 310016,China);Research Progress on Plant Physiology of Cold Resistance[J];Shandong Agricultural Sciences;2009-08
5 Li Hai-lin Yin xu-ming Long xiao-jun(Crop Physiology and Molecular Biology Laboratory,Hunan Agriculture University,Hunan Changsha 410128);Effects of chilling stress on physiological and biochemical indexes of chill-resistance in rice seedlings[J];Anhui Agricultural Science Bulletin;2006-11
6 Yang Xiaolan(Yancheng Normal College,Yan City,Jiangsu,224002);Advances of Research on Drought Resistant Gene Engineering in Plants[J];Anhui Agricultural Science Bulletin;2007-20
7 Ping Xiumin et al.(1Researth Institute of Subtropical Forestry,CAF,Fuyang 311400,China;2Faculty of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China);Tissue culture and rapid propagation of Guzmania lingulata[J];Anhui Agricultural Science Bulletin;2008-23
8 Chen Zhe et al.(Department of Life Science,Normal University,Kunming 650092,China);Research Progress of Gene Engineering in Plant Stress Tolerance[J];Anhui Agricultural Science Bulletin;2009-07
9 HAN Xiao-guang et al (Biology Department of Langfang Normal College,Langfang,Hebei 065000);Study on Multiple Shoot Induction and Plantlet Regeneration of Kale[J];Journal of Anhui Agricultural Sciences;2006-03
10 XIONG Yan et al(College of Plant Science and Technology,Tarim University,Alar,Xinjiang 843300);Research and Utilization of Cold Resistance of Chinese Wild Vitis[J];Journal of Anhui Agricultural Sciences;2007-11
China Proceedings of conference Full-text Database 2 Hits
1 TIAN Dan-qing GE Ya-ying PAN Gang-min LIU Xiao-jing SHEN Xiao-Ian ZHANG Zhi YU Yong-ming (Research & Development Centre of Flower,Zhejiang Academy of Agricultural Sciences,Hangzhou 311202);Effect of Cold Acclimation on Physiological Indexes in Anthurium under Cold Stress[A];[C];2011
2 Zhang Sipu~(1,2) Wang Liangju~1 (1.College of Horticulture,Nanjing Agricultural University Nanjing 210095; 2.Institute of Horticulture,Henan Academy of Agriculture Science Zhengzhou 450002);Cloning and Expression of Genes Related with Anthocyanidin Synthesis in Pomegranate(Punica granatum L.) fruits[A];[C];2010
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CUI Mei1,JIAO Qi-qing2,CHEN Xue-sen1,CHEN Xiao-liu1(1.College of Horticulture Science and Engineering,Shandong Agricultural University/ State Key Laboratory of Crop Biology, Taian 271018,China;2.Shandong Institute of Pomology,Taian 271000,China);Induction Culture of Callus from Apple Leaves[J];Shandong Agricultural Sciences;2012-03
2 XU Chun-bo et al(Grassland Research Institute of Chinese Academy of Agricultural Sciences,Hohhot,Inner Mongolia 010010);Research Progress on CBF Transcription Factor Applied to Genetic Engineering in China[J];Journal of Anhui Agricultural Sciences;2010-04
3 WANG Rong-ying et al(Meteorological Bureau in Hengshui,Hengshui,Hebei 053000);Impact of Climate Changes on Freezing Injury to Fruit Trees and Defensive Measures[J];Journal of Anhui Agricultural Sciences;2012-09
4 GUO Hong-guang et al(College of Agriculture,Hainan University,Haikou,Hainan 570228);Advance in the Studies on the Regulation and Functions of Plant MYB Transcription Factors[J];Journal of Anhui Agricultural Sciences;2012-20
5 PING Ji-cheng,SHAN Shou-ming,LIU Jing,WANG Zhen-ping(College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021);Effects of Polyamines and Urea Applied in Autumn on Storage Nutrition Content and Freezing Tolerance in Grapevine[J];Northern Horticulture;2011-17
6 WANG Shao-yun 1,ZHAO Jun1,WU Jin-hong 2,CHEN Lin1(1.College of Biological science and Engineering,Fuzhou University,Fuzhou 350002,China;2.College of Biology and Food Science,Shanghai Jiaotong University,Shanghai 290002,China);Research Progress in Antifreeze Proteins and Application in Food Industry[J];Journal of Beijing Technology and Business University(Natural Science Edition);2011-04
7 DING Cheng-long1,LIU Ying2,SHEN Yi-xin2,GU Hong-ru1(1.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing Jiangsu Province 210014,China;2.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing Jiangsu Province 210095,China);QTL Analysis of Traits Relating to Cold Resistance of Zoysia japonica[J];Acta Agrestia Sinica;2010-05
8 CHAI Qing,ZHONG Fang,LI Chun-jie,NAN Zhi-biao(College of Pastoral Agriculture Science and Technology,Lanzhou University;Key Laboratory of Grassland Argo-Ecosystem,Ministry of Agriculture,Lanzhou,Gansu Province 730020,China);Effects of Biofertilizer on Growth of Three Landscaping Grasses under Different Soil Moisture Contents[J];Acta Agrestia Sinica;2010-06
9 SHI Li-li,LI Bao-guo,QI Guo-hui,GUO Su-ping,ZHANG Xue-mei,QIU Zheng-fang,YANG Guang-shun(College of Forestry,Agricultural University of Hebei,Baoding 071000,China);Studies on the related physicochemical properties of different freezing injury walnut grafted seedlings[J];Journal of Agricultural University of Hebei;2011-05
10 WANG Yun-tao,YU Lin-qing,SA Ren(Grassland Research Institute,Chinese Academy of Agricultural Science,Hohhot 010010,China);Research progress of alfalfa cold resistance in China[J];Grassland and Turf;2012-03
China Proceedings of conference Full-text Database 2 Hits
1 Wang Rong-ying1,Li Chun-qiang2,Wu Yan1,Li Xin1(1.Meteorological Bureau in Hengshui,Hengshui 053000,China;2.Hebei Provincial Key Lab for Meteorological and Eco-Environment/Hebei Institute of Meteorological Science,Shijiazhuang 050021,China);Impact of Climate Change on Freezing Injury to Fruit Trees and Defensive Measures[A];[C];2012
2 Wang Yuanying,Luo Chenggang, Liu Guanshan,Li Yongping;Advances in Tobacco Breeding[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Zhang Zhihong 1,Wu Luping 1,Dai Hongyan 1,Wang Guoying 2,Zhao Tianyong 2,Bi Xiaoying 1,and Du Guodong 1 ( 1Department of Horticulture,Shenyang Agricultural University,Shenyang 110161; 2College of Biology,China Agricultural University,Beijing 1;Regeneration and Transformation in Vitro of the Strawberry Varieties[J];Acta Horticulturae Sinica;2001-03
2 DENG Xin [WT6BZ]([WT6BX]Institute of Botany, The Chinese Academy of Sciences, ]Beijing 100093) HU Wen_Yu (Shenyang Agricultural University, Shenyang 110161);Establishment of Regeneration and Genetic Transformation System of Strawberry Leaf[J];CHINESE BULLETIN OF BOTANY;2000-02
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