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《Acta Botanica Boreali-Occidentalia Sinica》 2007-03
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The Phylogenetic Relationships of Kengyilia Species Based on Evidences from ITS Sequences

ZHANG Li1,2,ZHOU Yong-hong2*,DING Chun-bang1,2,YANG Ruiwu1,2,FAN Xing2(1 College of Biology and Science,Sichuan Agricultural University,Yaan,Sichuan 625014,China;2 Key Laboratory of Crop Genetic Resources and Improvement,Ministry of Education,Sichuan Agricultural University,Yaan,Sichuan 625014,China)  
The entire sequences of ITS region of nuclear rDNA were analysed in 11 Kengyilia species.Phylogenetic relationships among 11 species in Kengyilia and Bromus tectorum L.as outgroup were estimated by maximum parsimony and neighbor-joining methods with PAUP4.0b10 software,and similar ITS trees were obtained with the two methods.The results showed:(1) the sequence of ITS in 11 species ranged from 596 bp to 601 bp in length.The G+C content varied from 61.20% to 62.44%.The genetic distances varied from 0.003 to 0.033,with an average of 0.015;(2) K.laxiflora and K.tahelacana were clustered together,located at the basal position of the tree with a bootstrap value 78% by maximum parsimony and 82% by neighbor-joining method;(3) The species with similar morphological characters and from the same areas or neighboring geographical regions were clustered together,respectively.It suggested that they had higher genetic similarities and closer relations;(4) The results based on ITS sequence analysis are basically comparable with those obtained from studies on morphology and cytology.It is an effective method to assess relationships among the species of Kengyilia.
【Fund】: 国家自然科学基金(30270099 30470135);; 教育部长江学者和创新团队发展计划(IRT0453);; 四川省教育厅、科技厅资助项目
【CateGory Index】: Q941;S543.9
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