Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Botanica Boreali-Occidentalia Sinica》 2007-05
Add to Favorite Get Latest Update

Cloning Full Length cDNA of Chinese Wild Vitis Novel Gene and Sequence Analysis

ZHAO Wei,XU Wei-rong,XU Yan,HE Ming-yang,WANG Yue-jin(College of Horticulture,Northwest A & F University,Key Laboratory of Horticulture Plant Germplasm and Genetic Improvement of China Agricultural Ministry,Yangling,Shaanxi 712100,China)  
According to an EST sequence of cDNA libarary from Vitis pesudoreticulata Baihe-35-1 inoculated with Uncinula necator,a pair of specific primers were designed and the SMART-RACE were performed with reverse transcription products of total RNA as the template.Concurrently bioinformatic methods were applied to analyze the obtained sequence and its induced amino acid sequence,including ORF,homologous sequences,physical and chemical properties,signal peptide,enzyme/ non-enzyme,subcellular localization and protein secondary structure.The results showed that the full length cDNA had an open reading frame of 584 bp with 107 bp 5′ untranslated regions and 162 bp 3′ untranslated regions.The deduced amino acid had 55% identity to Arabidopsis RNA/DNA binding protein,63% identity to Oryza galactosyltransferase,and the 8 to 82 amino acid was homologous with RNA recognition motif,which belongs to a typical functional domain.The protein was instable,Mr=21 801.21,pI=6.86,without signal peptide,and located to nucleus.The secondary structure was primarily composed of randon coil.
【Fund】: 国家自然科学基金资助项目(30571280)
【CateGory Index】: S663.1;Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 Li Jianfang1 Zhang Huimin1 Wu Minchen2(1 School of Food Science and Technology,Jiangnan University,Wuxi 214122;2 School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122);Bioinformatics Analysis of the Lipase from Penicillium cyclopium PG37[J];Biotechnology Bulletin;2010-03
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Bao Jinku Wu Chuanfang An Jie Gao Shun Zhao Xi Chang Liqing Rong Yanzhen Wang Chenji Chen FangΔ (College of Life Science, Sichuan University, Chengdu 610064,China);Molecular Cloning and Analysis of a Monocot Mannose-binding Agglutinin from Zephyranthes Grandiflora(Family Amaryllidaceae)[J];Journal of Biomedical Engineering;2004-05
2 in Chinese Native Wild Vitis L. Species Wang Yuejin He Puchao (Department of Horticulture,Northwestern Agricultural University,Yangling,Shaanxi 712100);Study on the Inheritance of Resistance to Powdery mildew[J];SCIENTIA AGRICUTURA SINICA;1997-01
3 HU Jian-Guan; ZHAO Xiang-Shan; LIU Jun; YUAN Zi-Qiang; YANG Jin-Shui (Institute of Genetics, Fudan University, Shanghai 200433);Isolation and Characterization of a cDNA Encoding Maize Cytosolic Malate Dehydrogenase[J];ACTA BOTANICA SINICA;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Hao-qin et al(Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212003 );Cloning and Sequence Analysis of Biological Clock Gene Bmtim2 in Bombyx mori L.[J];Journal of Anhui Agricultural Sciences;2008-17
2 LIU Hui-ning1, LI Hua2( 1. College of Horticulture and Gardens, Yangtze University, Jingzhou Hubei 434025, PRC;2. Enology College, Northwest Science and Technology University of Agriculture and Forestry, Yangling Shanxi 712100, PRC );Studies on The Resistance to Uncinula necator and Plasmopara Viticola of Vitis vinifera L.[J];Journal of Northeast Agricultural University;2004-03
3 ZHANG Jin-jin,WANG Yue-jin(1 College of Horticulture Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 College of Life Science,Shaanxi Normal University,Xi'an 710062,China);Cloning of Ribosomal RNA Gene in Chinese Wild Vitis Species by DDRT-PCR[J];Acta Botanica Boreali-occidentalia Sinica;2005-11
4 JIANG Rui hua, LIN Chang fa, SHEN Guo an, QIAN Min, WANG Dong, DU Xi ling, HUANG Ji, YANG Jin shui (Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433,China);Cloning of the Rice Cytoplasmic Malic Dehydrogenase Gene ( Rc MDH ) and Its Expression in E.coli[J];Journal of Fudan University;2002-01
5 Wang Yuejin and He Puchao(Department of Horticulture,Northwestern Agricultural University,Yangling,Shaanxi 712100);Study on the Inheritance of Resistance to Anthracnose of F1 Generation of Interspecific Hybrids of Vitis L.[J];;1998-01
6 Liu Huining and Zheng Qi (Department of Horticulture,Hubei Agricultural College,Jingzhou,Hubei434025China);Study on the Resistance to Uncinula necator of Several Grape Va rieties[J];Journal of Fruit Science;2002-06
7 GUO Yu qi et al . (Dept. of Agronomy,Luoyang Agricultural College,Luoyang,471003);Gene Expression and the Mechanisms of Heterosis[J];Journal of Henan Vocation-technical Teachers College;2001-02
8 LI Hua1, CHENG Yu-wen1, LI Pei-hong1 and LIU Shu-dong2 (1.College of Enology, Northwest A﹠F University, Yangling, Shanxi 712100; 2.Department of Agronomy, Northwest A﹠F University, Yangling, Shanxi 712100, China);Study on the Hereditary Rules of Auxiliary Shoot of Wild Grapes and Its F_1 Generation[J];Liquor-Making Science & Technology;2007-06
9 ZHANG Zhuan XUE Wei WANG Xiang-Kun SUN Chuan-Qing**(Department of Plant Genetics and Breeding,State Key Laboratory for Agrobiotechnology,Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Beijing Key Laboratory of Crop Genomics and Genetic Improvement,China Agricultural University,Beijing 100094,China);Studies on the Relationship between Heterosis and Gene Differential Expression Using Introgression Lines of Rice(Oryza sativa L.)[J];Journal of Agricultural Biotechnology;2005-06
10 HAO Wei WANG Yue-Jin WANG Xi-Ping XU Wei-Rong SUN Ma (Key Laboratory of Horticulture Plant Germplasm and Genetic Improvement, Ministry of Agriculture, College of Horticulture, Northwest Agriculture and Forestry University, Yangling 712100, China);Fusion Expression and Purification of Resveratrol Synthetic Enzyme Gene from Chinese wild Vitis pseudoreticulata and Preparation of Its Polyclonal Antibody[J];Journal of Agricultural Biotechnology;2006-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wang Zaigui,Wan Sailuo,Li Xunli(Anhui Agricultural University,College of life Science,Hefei 230031);Study of enzymic features of xylan enzyme of Trichoderma[J];Forest By-Product and Speciality in China;2007-01
2 XIAO Li, WANG Gui-xue, CHEN Guo-juan (1.College of Bioengineering, Chongqing University, Chongqing 400030, China; 2. Research Center of Applied Biotechnology, Chongqing University, Chongqing 400030, China);Preliminary Study of the Optimum Working Conditions on Xylanse During Process of Ramie Degumming[J];Journal of Chongqing University(Natural Science Edition);2004-06
3 HUANG Jun-li~1,LI Chang-jun~2,WANG Gui-xue~1 (1.College of Biological Engineering,Chonging University,Chongqing 400030,China;2.Chongqing Tabacco Corporation,Chongqing 400020,China);Xylanase Gene from Bacillus Subtilis B10[J];Journal of Chongqing University(Natural Science Edition);2006-06
4 Zhuang Meifang;A Study on Process of Biochemical Treatment of Linen Carded Yarn[J];Journal of Textile Research;1994-12
5 Liu Zhengchu et al;A Study on Industrial Application of A New Technique for Ramie Degunming by Microorganism[J];Journal of Textile Research;2001-02
6 He Wenming;Xylanase Application in US Kraft Mills[J];World Pulp and Paper;2003-05
7 LI Cai-e1,2,MA Yue-hui1,YE Shao-hui2(1.Institute of Animal Science,Chinese Academy of Agricultrual Science,Beijing 100094,China;2.College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China);The Application of Bioinformatics on Livestock and Poultry Genomics Study[J];China Animal Husbandry & Veterinary Medicine;2007-02
8 He Chengxin Zhang Hourui (Guangxi Institute of Botany,Yanshan,Guilin,Guangxi,541006);Advance in the Studies of Xylanolytic Enzymes[J];GUANGXI JOURNAL OF LIGHT INDUSTRY;1998-04
9 SHEN Pingxiang1,HUANG Jiang12**,ZHOU Linggui,LIAO Xingjiang1,LANG Shuyuan2(1.The Multimedia Laboratory of Morphology,Guiyang Medical College,Guiyang 550004,China;2.Department of Parasitology,Guiyang Medical University Guiyang 550004);Informatics Analysis of 60S Ribosomal Protein L10a Gene of Taenia asiatica[J];Journal of Guiyang Medical College;2007-04
10 Zhou Linggui,Huang Jiang,Shen Pinxiang,Liao Xingjiang,Lang Shuyuan,et al.Department of Parasitology,Guiyang Medical College,Guiyang 550004;Bioinformatic analysis of RNA polymerase Ⅱ subunit Gene in the Taenia asiatica[J];Guizhou Medical Journal;2007-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved