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《Acta Botanica Boreali-Occidentalia Sinica》 2008-01
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High Expression of hGM-CSF Induced by the cpcβ Gene Promoter in Anabaena sp.PCC 7120

WEI Lan-zhen(1,2),TAN Wei2,WANG Quan-xi(1,2)(1 School of Life Science,East China Normal University,Shanghai 200062,China;2 College of Life & Environment Sciences,Shanghai Normal University,Shanghai 200234,China)  
To increase the expression amount of foreign hGM-CSF in Anabaena sp.PCC 7120,the PpsbA promoter was replaced by the cpcβ gene promoter,originating from Synechococcus sp.PCC 7002.Western blot analysis showed that the expression level of hGM-CSF induced by the cpcβ gene promoter was about 90% more than that induced by the PpsbA promoter.Thus,the cpcβ gene promoter is a highly efficient promoter,and remarkably stimulates over-expression of hGM-CSF in Anabaena sp.PCC 7120.
【Fund】: 上海市高等学校科技基金重点项目(05ZZ15);; 上海市高校选拔培养优秀青年教师科研专项(RE569)
【CateGory Index】: Q943.2
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