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《Acta Botanica Boreali-Occidentalia Sinica》 2008-01
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Factors Influencing Pollen Germination and Tube Growth of Prunus armeniaca L.in vitro

HUANG Jia-xing1,WU Jie1,AN Jian-dong(1),TAN Ben-zhou2,XING Yan-hong3(1 Institute of Apiculture,Chinese Academy of Agricultural Sciences,Beijing 100093,China;2 College of Agriculture,Changjiang University,Jingzhou,Hubei 434023,China;3 Farming Service Center of Pinggu District,Beijing 101200,China)  
The paper reports factors influencing pollen germination and tube growth of Prunus armeniaca L.cv Katy in vitro.The results showed that the most suitable liquid culture medium for pollen germination and tube growth was 20% sugar+0.04% boric acid+0.01% CaCl2.In this culture medium,pollen germination rate was 68.76% and its tube length was 1 083.53 μm after 20 hours in culture.The suitable pH value of culture medium was 6.0.The higher or lower pH value would reduce the pollen germination and pollen tube growth.The best temperature for pollen germination and growth was 20℃.The effectiveness of four plant growth regulating substances on pollen germination and tube growth were different.Gibberellin(5~8 mg/L),Chlormequat chloride(10~150 mg/L) and paclobutrazol(5~10 mg/L) stimulated the pollen germination and tube growth.However,daminozide inhibited the pollen germination and tube growth.
【Fund】: 中国农业科学院所长基金(200603);; 国家科技支撑计划子课题(2006BAD06B04-2)
【CateGory Index】: S662.2
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