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《Acta Botanica Boreali-Occidentalia Sinica》 2008-11
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Identification of Chromosome and Fertility of BC_1 between Elymus canadensis and E.cylindricus

MA Yan-hong,YU Zhuo,LI Xiao-lei,LI Xiao-yu,LI Zao-zhe(Agronomy College,Inner Mongolia Agricultural University,Hohhot 010019,China)  
With Elymus canadensis L.(2n=4x=28) and Elymus cylindricus(Franch.) Honda(2n=6x=42) as tested plants,the chromosome number,chromosome pairing configuration,pollen fertility and seed set of BC1 were analyzed.The results showed that the chromosome numbers in above 85% root tip cell of BC1 plant are 28,which are tetraploid(2n=4x=28);the chromosome pairing behavior at metaphase Ⅰ in pollen mother cells of BC1 plant are regular,and the average chromosome pairing configurations are 0.04Ⅰ+13.98Ⅱ,and the frequency of ring bivalent was obviously more than rod bivalent;the pollen fertility and the seed set of BC1 plant are 84.83%and 70.98%respectively.It was proved that the fertility of BC1 plant had been restored.The above results provide a basis for selection and breeding of progenies.
【Fund】: 国家重点基础研究发展计划(973计划)项目(2007CB108901);; 国家自然科学基金(30560100)
【CateGory Index】: S54;Q943
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