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《Acta Botanica Boreali-Occidentalia Sinica》 2009-01
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AFLP Analysis on Dual Color Flower Gladiolus Mutants Induced by Radiation

LIU Chang-ming,ZHANG Xian,XU Qing,XIAN Feng(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China)  
The bulbs of the gladiolus cv.'Spic and span'were induced by different dose of 60Co γ and a great rang of different kinds of variation occurred in the progenies of receptor.The mutants with red-and-white color which induced by 75 GY were selected in M2 generations after observation of biological traits.AFLP molecular marker technology was used to compare these mutants with the control.The main results were as follows.50 of 64 selective primer pairs amplified 1 600 clear bands,112 of them were polymorphic loci which detected by 50 of 64 primer pairs.The polymorphic rate and Neis association coefficient were 13.08% and 93% respectively.The range of the polymorphic fragments was mainly 100~700 bp.The results indicated that the differences between the dual color flower mutant and the control were related to the changes of genetic material,some of them may be related to the genes involved in flower color formation.
【Fund】: 十一国家科技支撑计划(2006BA01A7-6-07);; 西北农林科技大学植物遗传育种专项(05YZ023-1)
【CateGory Index】: S682.24
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