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《Acta Botanica Boreali-Occidentalia Sinica》 2009-03
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Cloning and Characterization of A Vacuolar H~+-PPase Gene from Medicago truncatula

WANG Jian-wu1,2,SHEN Yu-hu1,WANG Pei1,2,WANG Hai-qing1(1 Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining 810001,China;2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China)  
A vacuolar H+-PPase cDNA,named MtVP1,was cloned from the legume model plant Medicago truncatula by EST in silico cloning and RT-PCR.Using Arabidopsis thaliana AVP1 cDNA sequence as a querying probe to search against Medicago truncatula EST database in GenBank,contig containing a full-length cDNA sequence was obtained by EST merging and proved with RT-PCR.The sequence analysis has revealed that MtVP1 contains a maximum open reading frame of 2 298 bp for 765 amino acids.The deduced amino acid sequence was highly similar to vacuolar H+-PPase from other plants,such as Vigna radiata and Arabidopsis thaliana,with an identity of 84%~93%.Computional analysis showed MtVP1 is a typical membrane protein with 13 trans-membrane domains and contained three conservative domains(CS1,CS2 and CS3) in vacuolar H+-PPase.Its sequence conservation and structure similarity with AVP1 implied that MtVP1 had similar function with AVP1.
【Fund】: 中国科学院知识创新工程方向性项目(KSCX2-YW-N-052);; 中国科学院“西部之光”项目(110040321)
【CateGory Index】: Q943.2
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【Co-citations】
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1 ZHAO Xiang-qiang (School of Life Sciences,Nantong University,Nantong,Jiangsu 226007);Advances in Studies on the Molecular Mechanism of Plant's Salt Tolerance[J];Journal of Anhui Agricultural Sciences;2009-17
2 XI Jie-jun,WU Guo-qiang,BAO Ai-ke,WANG Suo-min(College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China);Cloning and sequence analysis of a vacuolar H~+-PPase gene fragment from Zygophyllum xanthoxylum[J];Acta Prataculturae Sinica;2011-01
3 Hu Youzhen Wang Yan Zhang Fuchun College of Life Science and Technology,Xinjiang University,Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Urumqi,830046;Progress of the Correlation between Plant Proton Pump and Salt Tolerance[J];Molecular Plant Breeding;2009-06
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6 Gao Haibo Zhang Fuchun(Key Laboratory of Molecular Biology,College of Life Science and Technology,Xinjiang University,Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Urumqi 830046);Advances in Research on the Molecular Mechanism of Plant Salinity Tolerance and Morphological Characters of Chenopodiaceae[J];Biotechnology Bulletin;2008-04
7 GAO Hai-Bo,ZENG You-Ling,ZHANG Fu-Chun(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of LifeScience and Technology,Xinjiang University,Urumqi 830046,China);Callus Induction,Differentiation and Establishment of Plantlet Regeneration System of Salsola ferganica[J];Journal of Wuhan Botanical Research;2008-06
8 GUO Zhao-kui1,YANG Qian1,YAN Pei-qiang2,WAN Xiu-qing2 (1 Life Sciences & Engineering Dept,Harbin Institute of Technology,Harbin,Heilongjiang 150001,China;2 Heilongjiang Tobacco Research Institute,Mudanjiang,Heilongjiang 157011,China);Transcript analysis of K~+ uptake relative genes in AVP2 transgenic tobacco[J];Journal of Northwest A & F University(Natural Science Edition);2008-09
9 JIN Wei-Huan1,2,DONG Jian-Hui2,CHEN Ming2,*,CHEN Yao-Feng1,LI Lian-Cheng2,XU Zhao-Shi2,and MA You-Zhi2,* 1 College of Agronomy,Northwest A&F University,Yangling 712100,China;2 National Key Facility for Crop Gene Resources and Genetic Improvement /Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture /Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Physiological Mechanisms of Enhanced Uptake of Potassium Nutrition in EdHP1(H~+-pyrophosphatase) Transgenic Tobacco (Nicotiana tabacum)[J];Acta Agronomica Sinica;2010-10
10 YAO Shi-xiang,LAN Hai-yan (Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China);Strategies of cDNA Library Construction and Its Application in Halophytes[J];Xinjiang Agricultural Sciences;2008-06
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