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《Acta Botanica Boreali-Occidentalia Sinica》 2009-05
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Screen of SSR Markers from EST of Flax(Linum usitatissimum L.)

ZHANG Jian-ping1,WANG Bin2,ZHAO Li-juan3,WANG Li-min1,YANG Chong-qing2,XIE Rui-xia2,DANG Zhan-hai1(1 Institute of Crop Science,Gansu Academy of Agricultural Science,Lanzhou 730070,China;2 Agronomy College,Gansu Agricultural University,Lanzhou 730070,China;3 Institute of Bio-technology,Gansu Academy of Agricultural Science,Lanzhou 730070,China)  
In this study,222 SSR from 7941 ESTs in NCBI are obtained,representing 2.73% of total ESTs.Trinucleotide repeats,accounting for 72.1% of EST-SSRs,are dominant.Di-and Tri-nucleotide repeats are similar with frequency,accounting for 14.4% and 13.5% of SSRs,respectively.AGAA is the most frequent motif,about 67.67% in tetranucleotide repeats.18 pairs from 21 designed primer pairs of EST-SSRs show the amplification under a suitable PCR system in 10 flax germplasm.14 primers amplify clear bands with high SSR polymorphism Based on SSR markers,dendrogram analysis could divide 10 flax germplasm into 3 groups.The developed Flax SSR markers in this study could provide a useful molecular system to identify the genetic diversity and to construct a fingerprint map.
【Fund】: 现代农业产业技术体系专项资金资助(nycytx-22);; 甘肃省农业生物技术研究与应用开发项目(GNSW-2008-13);; 甘肃省自然科学科学基金项目(ZS021-A25-034-N);; 油料作物品种创新及产业化发展研究创新团队项目
【CateGory Index】: S563.2
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