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《Acta Botanica Boreali-Occidentalia Sinica》 2015-07
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Studies on Stigma Receptivity and Pollen Viability and Pollen Tube Growth of‘Chuanzao loquat'in the First Florescence

ZHANG Xiao;LIAO Ming'an;HE Jing;LIU Chunyang;MA Qianqian;YANG Daiyu;LU Chunyou;ZHOU Tingguo;DAI Shengyin;College of Horticulture,Sichuan Agricultural University;Hanyuan County Bureau of Science and Technology;Hanyuan County Bureau of Agriculture;  
In order to reveal the reason for low fruit setting rate of a new very precocious loquat line‘Chuanzao loquat'in the first florescence,we adopted the method of field experiment to observe flowering stage of‘Chuanzao loquat'in the first florescence.Stigma receptivity was measure by benzidine-H2O2,pollen vitality was tested by TTC method and pollen tube growth was observed by aniline blue staining method in this study.Results showed that:(1)The first florescence of'Chuanzao loquat'to enter beginning bloom stage was mid-July and full bloom stage was from late July to early Aug.The final bloom stage was from mid-Aug.to late Aug.The highest,lowest and average air temperature in the first florescence was 34.9℃,18.9 ℃ and 26.03 ℃,respectively.(2)The stigma with receptivity was on 0-4dafter flowering.The stigma with very strong receptivity and pollen with the highest viability was on 1dafter flowering,then the pollen viability rapidly reduced.(3)Pollen tubes had arrived in style base at 48 hand entered the ovuleat 96 hafter self-pollination,but very few.Accordingly,the combination of many factors such as short duration of stigma strong receptivity,pollen viability decreased rapidly,very few pollen tubes which could arrive at style base and enter the ovule and high temperature was the important reason of low fruit-set rate of‘Chuanzao loquat'in the first florescence.
【Fund】: 国家农业科技成果转化资金项目(2011GB2F000006);; 四川省雅安市和四川农业大学市校合作项目(00323303);; ‘双支计划’项目(03523531)
【CateGory Index】: Q944.42
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