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《Acta Botanica Boreali-Occidentalia Sinica》 2015-11
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Development of Molecular Markers Linked to the Yellow Seed Color Gene in Brassica juncea L.

LIU Lu;HUANG Zhen;LU Hong;LANG Li'na;ZHAO Na;XU Aixia;College of Agriculture,Northwest A&F University;  
Based on the previous genetic map of the yellow seed color gene,the sequences of Brassica rapa and Arabidopsis genomes were employed to design primers.Five primer pairs were from a B.rapaBAC clone KBrH105I15 and six pairs of IP primers were based on a sequence between At3g14120 and At3g29615 in Arabidopsis chromosome 3,which is a homologous region of yellow seed color gene.These 11 pairs of primers were used to amplify an F2 population consisting of 1 212 individuals which from ‘Wuqi yellow-seeded'and‘Wugong brown-seeded'mustard.As a result,Y12 from KBrH105I15and IP-6from At3g24180 were linked to the yellow seed color gene tightly,and Y12 was a co-dominant marker.These two markers were located on either side of the target gene at a distance of 0.2and 0.1cM.The genetic distances were shortened by 0.3and 0.2cM,respectively compared to the closest markers reported previously.The development of these two closer markers laid a good foundation for cloning the yellow seed color gene from yellow-seeded mustard.
【Fund】: 国家“863”项目(2011AA10A104);; 教育部博士点基金(20110204120015);; 杨凌示范区自然基金(2014NY-19);; 西北农林科技大学唐仲英育种基金
【CateGory Index】: S565.4
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