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《Acta Botanica Boreali-Occidentalia Sinica》 2016-05
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Genetic Transformation of the Phalaenopsis F3′5′H to OT Lily Robina

LIU Ailing;LIU Yali;LOU Qian;ZHANG Haiqin;State Key Laboratory of Crop Stress Biology in Arid Areas,Ministry of Agriculture Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,College of forestry;  
To obtain bluish Liliumspp.in direct breeding,we screened the suitable variety-OT lily Robina for genetic transformation.Here,both embryonic callus induced from filament and regenerated bulb scales of OT lily Robina were used as the transformation material.Agrobacterium-mediated transformation of Phalaenopsis F3′5′H was studied.The results showed:with the pre-culture 3d,OD_(600)=0.8,infection time 10 min,co-cultured for 3d,100μmol/L acetosyringone conditions,the stable transformation rate of regenerated bulb scales could reach to the highest 12.78%;however,embryogenic callus pre-culture 2d,OD_(600)=0.8,infection time 10 min,co-cultured 3d,with 100μmol/L acetosyringone conditions,which the stable transformation rate was the highest 12.22%.In all the conditions,the best hygromycin-resistant screening concentrations always was 20mg/L.PCR and reverse transcription PCR assay showed 9putative transgenic plants were obtained.Southern hybridization analysisfurther proved 6plants that the transgenic lilium flowers carry blue gene F3′5′H.The results provided technical support and material basis for the continuing development of novel bluish Liliumflowers.
【Fund】: 国家自然科学基金(31471905)
【CateGory Index】: S682.31
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