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《ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA》 1994-03
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HISTOCHEMISTRY OF BERBERINE OF COPTIS CHINENSIS FRANCH.RHIZOME

Lu Gaolian and Hu Zhenghai(Institute of Botany,Northwestern University,Xi’an 710069)  
For determing the distribution and concentration of berberine in Coptis rhizomes,sectionswere cut from the fresh rhizomes and placed immediately in the various reagents in the previousresearches.But in present research,the fresh rhizomes were cut into 0.2-0.3cm long and treated with Hg-KI to crystallize berberine in tissues before the sections,which could be protected for a long time,were cut by rotating microtome,and three results were obtained.(1)Abundant crystallization of berberine was occurred in the parenchyma of the cortex,the pith and the pithrays. The berberine was also detected in walls of the xylem constituents,the stone cells and thebast fibers,(2)A smaller number of berberine crystals were observed in the section of basal partof a main rhizome than in those of middle part and its branches ,and the amount of berberine innodal part is more than that in cylindrical part.(3)The sections of various age rhizomes showedthat berberine began to concentrate in cells of rhizome of 2-year-old plants,and then it increasedactually in each year until its amount was at top level in 6-year-old plants. According to previousconclusions,we had offered some valuable suggestions on cultivation and harvest of Coptis chinensis Franch.
【Fund】: 国家自然科学基金
【CateGory Index】: S567.520.1
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1 HU Lu-jie;YANG Chao-dong;YUAN Long-yi;LIU Zhi-xiong;DENG Chu-hong;TAN Wen-sheng;ZHANG Xia;College of Garden and Horticulture, Yangtze University;Lichuan City Agriculture Bureau;;Study on the Anatomy and Barrier Structure Spatial-temporal Developmental Characters of Adventitious Root of Camellia sinensis cv. Lichuanhong[J];湖北农业科学;2016-14
2 ZHANG Xia;HU Lu-jie;ZHOU Cun-yu;YANG Chao-dong;College of Gardening and Horticulture,Yangtze University;;Studies on Anatomy and Apoplastic Barrier Histochemistry Characters of Oenanthe javanica (Bl.)DC. Adapted to Wetland Environment[J];中国蔬菜;2016-07
3 YANG Chao-Dong;LI Shou-Feng;YAO Lan;AI Xun-Ru;CAI Xiao-Dong;ZHANG Xia;The College of Gardening and Horticulture,Yangtze University;Baokang Country Forestry Bureau;The College of Forestry and Horticulture,Hubei Minzu University;;A study of anatomical structure and apoplastic barrier characteristics of Hydrocotyle sibthorpioides[J];草业学报;2015-07
4 ZHAO Nan;LI Long-yun;BAI Zhi-chuan;College of Horticulture and Landscape Architecture,Southwest University;Chongqing Academy of Chinese Materia Medica;;Research Status and Prospect of Traditional Chinese Medicine Material Named Coptis Chinensis[J];重庆理工大学学报(自然科学);2015-01
5 ZHANG Xia;YANG Chao-dong;NING Guo-gui;College of Gardening and Horticulture, Yangtze University;College of Horticulture and Forestry Sciences, Huazhong Agricultural University;;The Developmental Comparison of Apoplastic Barriers in Cynodon dactylon and Paspalum distichum Roots[J];湖北农业科学;2013-20
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8 YANG Quan-yu1,LI Yong-sheng2,GAO Xiu-feng1, WANG Yan-jun1,JIN Ke1,ZHAO Rui2(1.West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,China;2.School of Chemical Engineering,Sichuan University,Chengdu 610065,China);Staining Effect of Fluorochrome Berberine Hydrochloride on Nucleic Acid in Gels[J];分析测试学报;2008-09
9 TUO Ya-qin,MU Xiao-qian* and LIANG Zong-suo(College of Life Sciences,Northwest A & F University,Yangling Shaanxi 712100,China);A Preliminary Study on the Best Gathering Time of Radix aconicum carmichaeli debx[J];西北农业学报;2007-02
10 YUAN Wang-jun1,ZHANG Wei-rui2,SHANG Fu-de2 *(1.Institute of Natural Products,Henan University,Henan Kaifeng 475001,China;2.College of Life Science,Henan University,Henan Kaifeng 475001,China);Study on the Anatomical Structure of Vegetative Organs of Coptis chinensis and Its Pertinence to Sciophyte Conditions[J];河南大学学报(自然科学版);2007-02
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