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Lu Gaolian and Hu Zhenghai(Institute of Botany,Northwestern University,Xi’an 710069)  
For determing the distribution and concentration of berberine in Coptis rhizomes,sectionswere cut from the fresh rhizomes and placed immediately in the various reagents in the previousresearches.But in present research,the fresh rhizomes were cut into 0.2-0.3cm long and treated with Hg-KI to crystallize berberine in tissues before the sections,which could be protected for a long time,were cut by rotating microtome,and three results were obtained.(1)Abundant crystallization of berberine was occurred in the parenchyma of the cortex,the pith and the pithrays. The berberine was also detected in walls of the xylem constituents,the stone cells and thebast fibers,(2)A smaller number of berberine crystals were observed in the section of basal partof a main rhizome than in those of middle part and its branches ,and the amount of berberine innodal part is more than that in cylindrical part.(3)The sections of various age rhizomes showedthat berberine began to concentrate in cells of rhizome of 2-year-old plants,and then it increasedactually in each year until its amount was at top level in 6-year-old plants. According to previousconclusions,we had offered some valuable suggestions on cultivation and harvest of Coptis chinensis Franch.
【Fund】: 国家自然科学基金
【CateGory Index】: S567.520.1
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