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《ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA》 1998-04
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TAXOL PRODUCTION IN TISSUE CULTURE OF TAXUS CHINENSIS VAR. MAIREI

Zhang Zongqin,Yang Jianying and Wu Yaowu ( Northwestern Institute of Botany,Shaanxi Province and Academia Sinica ,Yangling Shaanxi 712100)  
Fresh weight biomass of callus originated from Taxus chinensis var. mairei young stem explant on different media were significantly different according to Duncans multiple range test,but the dry weight biomass of calli had no difference.The proper concentration of NAA could be a suitable alternative of 2,4 D,a commonly used plant growth regulator but had risk of inducing cancer.The superior media were TA or B 5 supplemented with 1.0 mg/L NAA and 0.5 mg/L BA.TLC results of organical extractions of calli and concentrations of Si gel column chromatographic fractions indicated that taxol and 10 deacetylbaccatin Ⅲ were existed.
【CateGory Index】: S791.490.4
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