The effects of activated Kupffer cells on liver cell regeneration in cirrhotic rats
Chen Ping;Han Benli;Li Kun;Duan Henchun (Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University,Chongqing, 400038)
Objective: To observe the effects of activated Kupffer cells on liver cell regeneration, and to probe the mechanism of Kupffer cell regulation during liver regeneration. Methods: The cirrhotic rats after partial hepatectomy were used as the animal model. Hepatocytes and Kupffer cells were cultured in vitro and 3H-TDR and 3H-Leucine incorporation in the cultured cells were determined. Results: When the hepatocytes were cocultured with the Kupffer cell supernatant of the cirrhotic rats after operation, the hepatocyte DNA and protein synthesis functions were obviously decreased, however, when the liver cells were cocultured with Kupffer cell supernatant of the normal rats, these functions were increased significantly. When the KCs were cultured in the condition of 10 μg/ml LPS, KCs could obviously promote liver regeneration. Conclusion:Kupffer cells can both inhibit and accelerate liver regenertion, thus it is important to modulate the KC functions properly in order to enhance the regeneration of hepatocyte.